Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20496 and SM_b20497 are separated by 8 nucleotides SM_b20497 and SM_b20498 are separated by 30 nucleotides SM_b20498 and SM_b20499 are separated by 66 nucleotides
SM_b20496: SM_b20496 - GMC oxidoreductase, at 515,354 to 516,964
_b20496
SM_b20497: SM_b20497 - L-xylulose kinase, at 516,973 to 518,502
_b20497
SM_b20498: SM_b20498 - hypothetical protein, at 518,533 to 519,375
_b20498
SM_b20499: SM_b20499 - glycerol-3-phosphate dehydrogenase, at 519,442 to 521,175
_b20499
Position (kb)
516
517
518
519 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 516.010 kb on - strand, within SM_b20496 at 516.010 kb on - strand, within SM_b20496 at 516.010 kb on - strand, within SM_b20496 at 516.010 kb on - strand, within SM_b20496 at 516.010 kb on - strand, within SM_b20496 at 516.156 kb on - strand, within SM_b20496 at 516.386 kb on + strand, within SM_b20496 at 516.636 kb on + strand at 516.659 kb on - strand, within SM_b20496 at 516.659 kb on - strand, within SM_b20496 at 516.659 kb on - strand, within SM_b20496 at 516.659 kb on - strand, within SM_b20496 at 516.660 kb on + strand, within SM_b20496 at 516.660 kb on + strand, within SM_b20496 at 516.660 kb on + strand, within SM_b20496 at 516.661 kb on - strand, within SM_b20496 at 516.661 kb on - strand, within SM_b20496 at 516.661 kb on - strand, within SM_b20496 at 516.661 kb on - strand, within SM_b20496 at 516.790 kb on - strand, within SM_b20496 at 517.055 kb on + strand at 517.056 kb on - strand at 517.056 kb on - strand at 517.065 kb on - strand at 517.069 kb on - strand at 517.069 kb on - strand at 517.076 kb on + strand at 517.076 kb on + strand at 517.077 kb on - strand at 517.077 kb on - strand at 517.267 kb on + strand, within SM_b20497 at 517.421 kb on + strand, within SM_b20497 at 517.421 kb on + strand, within SM_b20497 at 517.422 kb on - strand, within SM_b20497 at 517.422 kb on - strand, within SM_b20497 at 517.422 kb on - strand, within SM_b20497 at 517.466 kb on + strand, within SM_b20497 at 517.475 kb on + strand, within SM_b20497 at 517.475 kb on + strand, within SM_b20497 at 517.475 kb on + strand, within SM_b20497 at 517.475 kb on + strand, within SM_b20497 at 517.475 kb on + strand, within SM_b20497 at 517.475 kb on + strand, within SM_b20497 at 517.476 kb on - strand, within SM_b20497 at 517.476 kb on - strand, within SM_b20497 at 517.628 kb on + strand, within SM_b20497 at 518.050 kb on - strand, within SM_b20497 at 518.138 kb on + strand, within SM_b20497 at 518.246 kb on + strand, within SM_b20497 at 518.246 kb on + strand, within SM_b20497 at 518.247 kb on - strand, within SM_b20497 at 518.247 kb on - strand, within SM_b20497 at 518.316 kb on + strand, within SM_b20497 at 518.316 kb on + strand, within SM_b20497 at 518.317 kb on - strand, within SM_b20497 at 518.317 kb on - strand, within SM_b20497 at 518.488 kb on + strand at 518.492 kb on + strand at 518.492 kb on + strand at 518.492 kb on + strand at 518.493 kb on - strand at 518.493 kb on - strand at 518.493 kb on - strand at 518.547 kb on - strand at 518.633 kb on + strand, within SM_b20498 at 518.633 kb on + strand, within SM_b20498 at 518.648 kb on + strand, within SM_b20498 at 518.648 kb on + strand, within SM_b20498 at 518.648 kb on + strand, within SM_b20498 at 518.876 kb on + strand, within SM_b20498 at 518.876 kb on + strand, within SM_b20498 at 518.877 kb on - strand, within SM_b20498 at 518.918 kb on - strand, within SM_b20498 at 518.936 kb on + strand, within SM_b20498 at 518.936 kb on + strand, within SM_b20498 at 518.936 kb on + strand, within SM_b20498 at 518.936 kb on + strand, within SM_b20498 at 519.119 kb on + strand, within SM_b20498 at 519.120 kb on - strand, within SM_b20498 at 519.363 kb on + strand at 519.364 kb on - strand at 519.377 kb on + strand at 519.378 kb on + strand at 519.379 kb on - strand at 519.379 kb on - strand at 519.462 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 516,010 - SM_b20496 0.41 -3.3 516,010 - SM_b20496 0.41 +0.5 516,010 - SM_b20496 0.41 +1.9 516,010 - SM_b20496 0.41 -1.2 516,010 - SM_b20496 0.41 +0.3 516,156 - SM_b20496 0.50 -1.3 516,386 + SM_b20496 0.64 -2.7 516,636 + +0.9 516,659 - SM_b20496 0.81 -1.0 516,659 - SM_b20496 0.81 +1.2 516,659 - SM_b20496 0.81 +2.1 516,659 - SM_b20496 0.81 -3.9 516,660 + SM_b20496 0.81 +0.8 516,660 + SM_b20496 0.81 +2.5 516,660 + SM_b20496 0.81 -4.2 516,661 - SM_b20496 0.81 -1.8 516,661 - SM_b20496 0.81 +4.4 516,661 - SM_b20496 0.81 -2.7 516,661 - SM_b20496 0.81 -0.7 516,790 - SM_b20496 0.89 +1.9 517,055 + -0.7 517,056 - -0.1 517,056 - -0.0 517,065 - +1.0 517,069 - +0.3 517,069 - -0.5 517,076 + +0.3 517,076 + +0.1 517,077 - +0.2 517,077 - +0.7 517,267 + SM_b20497 0.19 -0.7 517,421 + SM_b20497 0.29 +0.1 517,421 + SM_b20497 0.29 +0.4 517,422 - SM_b20497 0.29 +0.8 517,422 - SM_b20497 0.29 -1.5 517,422 - SM_b20497 0.29 -4.6 517,466 + SM_b20497 0.32 -0.7 517,475 + SM_b20497 0.33 -4.3 517,475 + SM_b20497 0.33 +1.0 517,475 + SM_b20497 0.33 +0.7 517,475 + SM_b20497 0.33 +1.8 517,475 + SM_b20497 0.33 +1.7 517,475 + SM_b20497 0.33 -0.4 517,476 - SM_b20497 0.33 +1.3 517,476 - SM_b20497 0.33 -0.0 517,628 + SM_b20497 0.43 -0.0 518,050 - SM_b20497 0.70 +1.7 518,138 + SM_b20497 0.76 +0.1 518,246 + SM_b20497 0.83 +0.8 518,246 + SM_b20497 0.83 +0.9 518,247 - SM_b20497 0.83 +0.7 518,247 - SM_b20497 0.83 +1.9 518,316 + SM_b20497 0.88 -0.1 518,316 + SM_b20497 0.88 -2.4 518,317 - SM_b20497 0.88 +0.5 518,317 - SM_b20497 0.88 -1.1 518,488 + -1.7 518,492 + +0.1 518,492 + +0.5 518,492 + +0.1 518,493 - +0.6 518,493 - +3.7 518,493 - +1.3 518,547 - -2.6 518,633 + SM_b20498 0.12 +0.6 518,633 + SM_b20498 0.12 -1.6 518,648 + SM_b20498 0.14 -1.3 518,648 + SM_b20498 0.14 +0.9 518,648 + SM_b20498 0.14 -1.7 518,876 + SM_b20498 0.41 -0.1 518,876 + SM_b20498 0.41 -3.6 518,877 - SM_b20498 0.41 +0.2 518,918 - SM_b20498 0.46 -4.6 518,936 + SM_b20498 0.48 +0.1 518,936 + SM_b20498 0.48 +0.5 518,936 + SM_b20498 0.48 -0.8 518,936 + SM_b20498 0.48 -2.1 519,119 + SM_b20498 0.70 -1.4 519,120 - SM_b20498 0.70 +0.4 519,363 + -0.4 519,364 - -1.5 519,377 + +0.1 519,378 + -0.6 519,379 - +0.9 519,379 - -0.2 519,462 + -0.4
Or see this region's nucleotide sequence