Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20487 and SM_b20488 are separated by 2 nucleotides SM_b20488 and SM_b20489 are separated by 44 nucleotides SM_b20489 and SM_b20490 overlap by 8 nucleotides SM_b20490 and SM_b22020 are separated by 107 nucleotides
SM_b20487: SM_b20487 - sugar ABC transporter permease, at 503,453 to 504,451
_b20487
SM_b20488: SM_b20488 - hypothetical protein, at 504,454 to 505,302
_b20488
SM_b20489: SM_b20489 - carbohydrate kinase, at 505,347 to 506,819
_b20489
SM_b20490: SM_b20490 - L-fuculose phosphate aldolase, at 506,812 to 507,510
_b20490
SM_b22020: SM_b22020 - hypothetical protein, at 507,618 to 508,478
_b22020
Position (kb) 505
506
507 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 504.475 kb on - strand at 504.475 kb on - strand at 504.475 kb on - strand at 504.475 kb on - strand at 504.475 kb on - strand at 504.475 kb on - strand at 504.541 kb on + strand, within SM_b20488 at 504.541 kb on + strand, within SM_b20488 at 504.541 kb on + strand, within SM_b20488 at 504.542 kb on - strand, within SM_b20488 at 504.542 kb on - strand, within SM_b20488 at 504.763 kb on + strand, within SM_b20488 at 504.763 kb on + strand, within SM_b20488 at 504.763 kb on + strand, within SM_b20488 at 504.764 kb on - strand, within SM_b20488 at 504.812 kb on - strand, within SM_b20488 at 504.819 kb on + strand, within SM_b20488 at 504.985 kb on - strand, within SM_b20488 at 504.988 kb on + strand, within SM_b20488 at 504.988 kb on + strand, within SM_b20488 at 504.988 kb on + strand, within SM_b20488 at 504.989 kb on - strand, within SM_b20488 at 505.026 kb on + strand, within SM_b20488 at 505.055 kb on - strand, within SM_b20488 at 505.198 kb on + strand, within SM_b20488 at 505.199 kb on - strand, within SM_b20488 at 505.199 kb on - strand, within SM_b20488 at 505.695 kb on + strand, within SM_b20489 at 505.696 kb on - strand, within SM_b20489 at 505.696 kb on - strand, within SM_b20489 at 505.698 kb on - strand, within SM_b20489 at 505.773 kb on + strand, within SM_b20489 at 505.774 kb on - strand, within SM_b20489 at 505.822 kb on - strand, within SM_b20489 at 505.822 kb on - strand, within SM_b20489 at 505.840 kb on - strand, within SM_b20489 at 506.084 kb on - strand, within SM_b20489 at 506.245 kb on + strand, within SM_b20489 at 506.467 kb on - strand, within SM_b20489 at 506.467 kb on - strand, within SM_b20489 at 506.467 kb on - strand, within SM_b20489 at 506.616 kb on + strand, within SM_b20489 at 506.617 kb on - strand, within SM_b20489 at 506.965 kb on + strand, within SM_b20490 at 506.966 kb on - strand, within SM_b20490 at 507.016 kb on + strand, within SM_b20490 at 507.016 kb on + strand, within SM_b20490 at 507.016 kb on + strand, within SM_b20490 at 507.016 kb on + strand, within SM_b20490 at 507.016 kb on + strand, within SM_b20490 at 507.017 kb on - strand, within SM_b20490 at 507.017 kb on - strand, within SM_b20490 at 507.017 kb on - strand, within SM_b20490 at 507.017 kb on - strand, within SM_b20490 at 507.017 kb on - strand, within SM_b20490 at 507.112 kb on + strand, within SM_b20490 at 507.113 kb on - strand, within SM_b20490 at 507.160 kb on + strand, within SM_b20490 at 507.160 kb on + strand, within SM_b20490 at 507.205 kb on + strand, within SM_b20490 at 507.296 kb on + strand, within SM_b20490 at 507.364 kb on + strand, within SM_b20490 at 507.364 kb on + strand, within SM_b20490 at 507.365 kb on - strand, within SM_b20490 at 507.365 kb on - strand, within SM_b20490 at 507.365 kb on - strand, within SM_b20490 at 507.365 kb on - strand, within SM_b20490 at 507.619 kb on + strand at 507.620 kb on - strand at 507.620 kb on - strand at 507.628 kb on + strand at 507.771 kb on - strand, within SM_b22020 at 507.775 kb on - strand, within SM_b22020 at 507.775 kb on - strand, within SM_b22020
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 504,475 - -1.7 504,475 - +0.0 504,475 - +0.6 504,475 - -0.1 504,475 - -0.4 504,475 - -2.9 504,541 + SM_b20488 0.10 +1.3 504,541 + SM_b20488 0.10 -0.9 504,541 + SM_b20488 0.10 -0.4 504,542 - SM_b20488 0.10 +2.3 504,542 - SM_b20488 0.10 +1.0 504,763 + SM_b20488 0.36 +1.2 504,763 + SM_b20488 0.36 -2.3 504,763 + SM_b20488 0.36 -0.2 504,764 - SM_b20488 0.37 +0.6 504,812 - SM_b20488 0.42 -1.5 504,819 + SM_b20488 0.43 -1.0 504,985 - SM_b20488 0.63 -1.9 504,988 + SM_b20488 0.63 +0.9 504,988 + SM_b20488 0.63 +0.9 504,988 + SM_b20488 0.63 +2.4 504,989 - SM_b20488 0.63 +0.5 505,026 + SM_b20488 0.67 -0.1 505,055 - SM_b20488 0.71 +0.4 505,198 + SM_b20488 0.88 -2.9 505,199 - SM_b20488 0.88 +2.6 505,199 - SM_b20488 0.88 +1.8 505,695 + SM_b20489 0.24 -0.4 505,696 - SM_b20489 0.24 +0.7 505,696 - SM_b20489 0.24 +0.6 505,698 - SM_b20489 0.24 -0.3 505,773 + SM_b20489 0.29 -3.5 505,774 - SM_b20489 0.29 -0.2 505,822 - SM_b20489 0.32 -2.3 505,822 - SM_b20489 0.32 +0.2 505,840 - SM_b20489 0.33 -0.8 506,084 - SM_b20489 0.50 +0.1 506,245 + SM_b20489 0.61 +2.2 506,467 - SM_b20489 0.76 +1.9 506,467 - SM_b20489 0.76 +1.3 506,467 - SM_b20489 0.76 -2.1 506,616 + SM_b20489 0.86 -1.0 506,617 - SM_b20489 0.86 +2.2 506,965 + SM_b20490 0.22 -1.5 506,966 - SM_b20490 0.22 +2.1 507,016 + SM_b20490 0.29 -2.0 507,016 + SM_b20490 0.29 -2.9 507,016 + SM_b20490 0.29 +0.3 507,016 + SM_b20490 0.29 +1.0 507,016 + SM_b20490 0.29 -1.1 507,017 - SM_b20490 0.29 -2.9 507,017 - SM_b20490 0.29 +1.3 507,017 - SM_b20490 0.29 +0.9 507,017 - SM_b20490 0.29 -0.5 507,017 - SM_b20490 0.29 -0.4 507,112 + SM_b20490 0.43 -0.0 507,113 - SM_b20490 0.43 -0.3 507,160 + SM_b20490 0.50 +0.1 507,160 + SM_b20490 0.50 -3.2 507,205 + SM_b20490 0.56 +0.3 507,296 + SM_b20490 0.69 -2.9 507,364 + SM_b20490 0.79 +0.1 507,364 + SM_b20490 0.79 +0.4 507,365 - SM_b20490 0.79 +1.0 507,365 - SM_b20490 0.79 +2.7 507,365 - SM_b20490 0.79 +1.4 507,365 - SM_b20490 0.79 -0.5 507,619 + -1.3 507,620 - +0.4 507,620 - +0.1 507,628 + +0.1 507,771 - SM_b22020 0.18 +1.0 507,775 - SM_b22020 0.18 -3.0 507,775 - SM_b22020 0.18 -0.4
Or see this region's nucleotide sequence