Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20485 and SM_b20486 overlap by 8 nucleotides SM_b20486 and SM_b20487 overlap by 4 nucleotides SM_b20487 and SM_b20488 are separated by 2 nucleotides SM_b20488 and SM_b20489 are separated by 44 nucleotides
SM_b20485: SM_b20485 - sugar ABC transporter ATP-binding protein, at 500,933 to 502,462
_b20485
SM_b20486: SM_b20486 - sugar ABC transporter permease, at 502,455 to 503,456
_b20486
SM_b20487: SM_b20487 - sugar ABC transporter permease, at 503,453 to 504,451
_b20487
SM_b20488: SM_b20488 - hypothetical protein, at 504,454 to 505,302
_b20488
SM_b20489: SM_b20489 - carbohydrate kinase, at 505,347 to 506,819
_b20489
Position (kb)
503
504
505 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 502.454 kb on + strand at 502.454 kb on + strand at 502.455 kb on - strand at 502.460 kb on + strand at 502.460 kb on + strand at 502.460 kb on + strand at 502.515 kb on - strand at 502.650 kb on - strand, within SM_b20486 at 502.684 kb on - strand, within SM_b20486 at 502.842 kb on - strand, within SM_b20486 at 502.845 kb on + strand, within SM_b20486 at 502.845 kb on + strand, within SM_b20486 at 502.846 kb on - strand at 502.887 kb on + strand, within SM_b20486 at 502.950 kb on - strand, within SM_b20486 at 502.950 kb on - strand, within SM_b20486 at 502.950 kb on - strand, within SM_b20486 at 503.046 kb on + strand, within SM_b20486 at 503.046 kb on + strand, within SM_b20486 at 503.046 kb on + strand, within SM_b20486 at 503.046 kb on + strand, within SM_b20486 at 503.094 kb on - strand, within SM_b20486 at 503.169 kb on + strand, within SM_b20486 at 503.170 kb on - strand, within SM_b20486 at 503.170 kb on - strand, within SM_b20486 at 504.050 kb on + strand, within SM_b20487 at 504.050 kb on + strand, within SM_b20487 at 504.051 kb on - strand, within SM_b20487 at 504.051 kb on - strand, within SM_b20487 at 504.051 kb on - strand, within SM_b20487 at 504.051 kb on - strand, within SM_b20487 at 504.053 kb on - strand, within SM_b20487 at 504.053 kb on - strand, within SM_b20487 at 504.053 kb on - strand, within SM_b20487 at 504.123 kb on - strand, within SM_b20487 at 504.210 kb on - strand, within SM_b20487 at 504.210 kb on - strand, within SM_b20487 at 504.475 kb on - strand at 504.475 kb on - strand at 504.475 kb on - strand at 504.475 kb on - strand at 504.475 kb on - strand at 504.475 kb on - strand at 504.541 kb on + strand, within SM_b20488 at 504.541 kb on + strand, within SM_b20488 at 504.541 kb on + strand, within SM_b20488 at 504.542 kb on - strand, within SM_b20488 at 504.542 kb on - strand, within SM_b20488 at 504.763 kb on + strand, within SM_b20488 at 504.763 kb on + strand, within SM_b20488 at 504.763 kb on + strand, within SM_b20488 at 504.764 kb on - strand, within SM_b20488 at 504.812 kb on - strand, within SM_b20488 at 504.819 kb on + strand, within SM_b20488 at 504.985 kb on - strand, within SM_b20488 at 504.988 kb on + strand, within SM_b20488 at 504.988 kb on + strand, within SM_b20488 at 504.988 kb on + strand, within SM_b20488 at 504.989 kb on - strand, within SM_b20488 at 505.026 kb on + strand, within SM_b20488 at 505.055 kb on - strand, within SM_b20488 at 505.198 kb on + strand, within SM_b20488 at 505.199 kb on - strand, within SM_b20488 at 505.199 kb on - strand, within SM_b20488
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 502,454 + +1.3 502,454 + -1.7 502,455 - -2.1 502,460 + -1.9 502,460 + +2.0 502,460 + -0.3 502,515 - +0.8 502,650 - SM_b20486 0.19 -1.9 502,684 - SM_b20486 0.23 +0.2 502,842 - SM_b20486 0.39 +0.3 502,845 + SM_b20486 0.39 -1.2 502,845 + SM_b20486 0.39 -0.1 502,846 - +0.9 502,887 + SM_b20486 0.43 -0.7 502,950 - SM_b20486 0.49 +0.4 502,950 - SM_b20486 0.49 +0.4 502,950 - SM_b20486 0.49 -1.5 503,046 + SM_b20486 0.59 +0.2 503,046 + SM_b20486 0.59 -0.3 503,046 + SM_b20486 0.59 +1.5 503,046 + SM_b20486 0.59 -0.1 503,094 - SM_b20486 0.64 +0.4 503,169 + SM_b20486 0.71 +0.4 503,170 - SM_b20486 0.71 +0.3 503,170 - SM_b20486 0.71 -2.1 504,050 + SM_b20487 0.60 -1.7 504,050 + SM_b20487 0.60 +0.2 504,051 - SM_b20487 0.60 +0.3 504,051 - SM_b20487 0.60 -0.7 504,051 - SM_b20487 0.60 +2.1 504,051 - SM_b20487 0.60 -1.9 504,053 - SM_b20487 0.60 -0.8 504,053 - SM_b20487 0.60 -2.8 504,053 - SM_b20487 0.60 -1.2 504,123 - SM_b20487 0.67 +0.4 504,210 - SM_b20487 0.76 -0.9 504,210 - SM_b20487 0.76 +0.0 504,475 - -1.7 504,475 - +0.0 504,475 - +0.6 504,475 - -0.1 504,475 - -0.4 504,475 - -2.9 504,541 + SM_b20488 0.10 +1.3 504,541 + SM_b20488 0.10 -0.9 504,541 + SM_b20488 0.10 -0.4 504,542 - SM_b20488 0.10 +2.3 504,542 - SM_b20488 0.10 +1.0 504,763 + SM_b20488 0.36 +1.2 504,763 + SM_b20488 0.36 -2.3 504,763 + SM_b20488 0.36 -0.2 504,764 - SM_b20488 0.37 +0.6 504,812 - SM_b20488 0.42 -1.5 504,819 + SM_b20488 0.43 -1.0 504,985 - SM_b20488 0.63 -1.9 504,988 + SM_b20488 0.63 +0.9 504,988 + SM_b20488 0.63 +0.9 504,988 + SM_b20488 0.63 +2.4 504,989 - SM_b20488 0.63 +0.5 505,026 + SM_b20488 0.67 -0.1 505,055 - SM_b20488 0.71 +0.4 505,198 + SM_b20488 0.88 -2.9 505,199 - SM_b20488 0.88 +2.6 505,199 - SM_b20488 0.88 +1.8
Or see this region's nucleotide sequence