Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20430 and SM_b20431 overlap by 1 nucleotides SM_b20431 and SM_b20432 overlap by 4 nucleotides SM_b20432 and SM_b20433 overlap by 4 nucleotides
SM_b20430: SM_b20430 - amino acid ABC transporter permease, at 444,905 to 445,567
_b20430
SM_b20431: SM_b20431 - arylmalonate decarboxylase, at 445,567 to 446,352
_b20431
SM_b20432: SM_b20432 - threonine dehydratase, at 446,349 to 447,353
_b20432
SM_b20433: SM_b20433 - ectoine utilization protein EutC, at 447,350 to 448,339
_b20433
Position (kb) 446
447
448 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 445.359 kb on - strand, within SM_b20430 at 445.447 kb on + strand, within SM_b20430 at 445.448 kb on - strand, within SM_b20430 at 445.448 kb on - strand, within SM_b20430 at 445.451 kb on + strand, within SM_b20430 at 445.451 kb on + strand, within SM_b20430 at 445.452 kb on - strand, within SM_b20430 at 445.458 kb on - strand, within SM_b20430 at 445.458 kb on - strand, within SM_b20430 at 445.498 kb on + strand, within SM_b20430 at 445.633 kb on - strand at 445.669 kb on - strand, within SM_b20431 at 445.721 kb on - strand, within SM_b20431 at 445.721 kb on - strand, within SM_b20431 at 445.739 kb on - strand, within SM_b20431 at 445.840 kb on + strand, within SM_b20431 at 445.840 kb on + strand, within SM_b20431 at 445.841 kb on - strand, within SM_b20431 at 445.841 kb on - strand, within SM_b20431 at 445.997 kb on - strand, within SM_b20431 at 446.030 kb on - strand, within SM_b20431 at 446.251 kb on + strand, within SM_b20431 at 446.417 kb on - strand at 446.417 kb on - strand at 446.420 kb on + strand at 446.480 kb on - strand, within SM_b20432 at 446.508 kb on - strand, within SM_b20432 at 446.645 kb on + strand, within SM_b20432 at 446.884 kb on + strand, within SM_b20432 at 446.959 kb on + strand, within SM_b20432 at 446.959 kb on + strand, within SM_b20432 at 446.959 kb on + strand, within SM_b20432 at 446.959 kb on + strand, within SM_b20432 at 446.960 kb on - strand, within SM_b20432 at 447.121 kb on + strand, within SM_b20432 at 447.121 kb on + strand, within SM_b20432 at 447.121 kb on + strand, within SM_b20432 at 447.122 kb on - strand, within SM_b20432 at 447.122 kb on - strand, within SM_b20432 at 447.269 kb on + strand at 447.270 kb on - strand at 447.270 kb on - strand at 447.531 kb on - strand, within SM_b20433 at 447.531 kb on - strand, within SM_b20433 at 447.531 kb on - strand, within SM_b20433 at 447.956 kb on - strand, within SM_b20433 at 448.085 kb on + strand, within SM_b20433 at 448.086 kb on - strand, within SM_b20433 at 448.322 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 445,359 - SM_b20430 0.68 -1.4 445,447 + SM_b20430 0.82 +0.9 445,448 - SM_b20430 0.82 +0.6 445,448 - SM_b20430 0.82 -2.7 445,451 + SM_b20430 0.82 -1.9 445,451 + SM_b20430 0.82 +0.9 445,452 - SM_b20430 0.83 -0.5 445,458 - SM_b20430 0.83 +1.5 445,458 - SM_b20430 0.83 +2.2 445,498 + SM_b20430 0.89 +1.2 445,633 - +0.3 445,669 - SM_b20431 0.13 -0.3 445,721 - SM_b20431 0.20 -0.3 445,721 - SM_b20431 0.20 -0.7 445,739 - SM_b20431 0.22 +1.0 445,840 + SM_b20431 0.35 -0.3 445,840 + SM_b20431 0.35 +1.1 445,841 - SM_b20431 0.35 +0.3 445,841 - SM_b20431 0.35 -1.7 445,997 - SM_b20431 0.55 +1.6 446,030 - SM_b20431 0.59 +0.6 446,251 + SM_b20431 0.87 -0.5 446,417 - -0.9 446,417 - +0.8 446,420 + +0.0 446,480 - SM_b20432 0.13 +2.8 446,508 - SM_b20432 0.16 -2.4 446,645 + SM_b20432 0.29 +0.0 446,884 + SM_b20432 0.53 -0.1 446,959 + SM_b20432 0.61 -1.0 446,959 + SM_b20432 0.61 +0.0 446,959 + SM_b20432 0.61 +0.6 446,959 + SM_b20432 0.61 -0.9 446,960 - SM_b20432 0.61 -0.4 447,121 + SM_b20432 0.77 -1.2 447,121 + SM_b20432 0.77 +0.7 447,121 + SM_b20432 0.77 -3.0 447,122 - SM_b20432 0.77 +0.0 447,122 - SM_b20432 0.77 -0.6 447,269 + +0.9 447,270 - -0.4 447,270 - -1.6 447,531 - SM_b20433 0.18 -0.4 447,531 - SM_b20433 0.18 +2.1 447,531 - SM_b20433 0.18 +1.4 447,956 - SM_b20433 0.61 +0.2 448,085 + SM_b20433 0.74 +1.2 448,086 - SM_b20433 0.74 -2.4 448,322 - -0.1
Or see this region's nucleotide sequence