Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20407 and SM_b20408 are separated by 3 nucleotides SM_b20408 and SM_b20409 overlap by 4 nucleotides SM_b20409 and SM_b20410 are separated by 68 nucleotides
SM_b20407: SM_b20407 - 5-oxoprolinase, at 422,142 to 424,343
_b20407
SM_b20408: SM_b20408 - hypothetical protein, at 424,347 to 424,844
_b20408
SM_b20409: SM_b20409 - 3-oxoacyl-ACP reductase, at 424,841 to 425,671
_b20409
SM_b20410: SM_b20410 - glycine-betaine-binding protein, at 425,740 to 426,678
_b20410
Position (kb) 424
425 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 423.522 kb on + strand, within SM_b20407 at 423.522 kb on + strand, within SM_b20407 at 423.522 kb on + strand, within SM_b20407 at 423.522 kb on + strand, within SM_b20407 at 423.523 kb on - strand, within SM_b20407 at 423.523 kb on - strand, within SM_b20407 at 423.523 kb on - strand, within SM_b20407 at 423.523 kb on - strand, within SM_b20407 at 423.591 kb on + strand, within SM_b20407 at 423.591 kb on + strand, within SM_b20407 at 423.591 kb on + strand, within SM_b20407 at 423.591 kb on + strand, within SM_b20407 at 423.592 kb on - strand, within SM_b20407 at 423.592 kb on - strand, within SM_b20407 at 423.701 kb on - strand, within SM_b20407 at 423.840 kb on - strand, within SM_b20407 at 423.882 kb on + strand, within SM_b20407 at 423.882 kb on + strand, within SM_b20407 at 423.915 kb on + strand, within SM_b20407 at 423.915 kb on + strand, within SM_b20407 at 423.916 kb on - strand, within SM_b20407 at 423.916 kb on - strand, within SM_b20407 at 423.916 kb on - strand, within SM_b20407 at 423.916 kb on - strand, within SM_b20407 at 423.916 kb on - strand, within SM_b20407 at 423.916 kb on - strand, within SM_b20407 at 423.916 kb on - strand, within SM_b20407 at 423.916 kb on - strand, within SM_b20407 at 423.960 kb on + strand, within SM_b20407 at 423.960 kb on + strand, within SM_b20407 at 423.962 kb on + strand, within SM_b20407 at 423.963 kb on - strand, within SM_b20407 at 423.963 kb on - strand, within SM_b20407 at 424.360 kb on - strand at 424.360 kb on - strand at 424.431 kb on + strand, within SM_b20408 at 424.432 kb on - strand, within SM_b20408 at 424.432 kb on - strand, within SM_b20408 at 424.432 kb on - strand, within SM_b20408 at 424.432 kb on - strand, within SM_b20408 at 424.432 kb on - strand, within SM_b20408 at 424.432 kb on - strand, within SM_b20408 at 424.433 kb on + strand, within SM_b20408 at 424.433 kb on + strand, within SM_b20408 at 424.434 kb on - strand, within SM_b20408 at 424.458 kb on + strand, within SM_b20408 at 424.591 kb on - strand, within SM_b20408 at 424.610 kb on + strand, within SM_b20408 at 424.948 kb on - strand, within SM_b20409 at 424.948 kb on - strand, within SM_b20409 at 425.061 kb on - strand, within SM_b20409 at 425.061 kb on - strand, within SM_b20409 at 425.205 kb on + strand, within SM_b20409 at 425.205 kb on + strand, within SM_b20409 at 425.205 kb on + strand, within SM_b20409 at 425.206 kb on - strand, within SM_b20409 at 425.206 kb on - strand, within SM_b20409 at 425.305 kb on + strand, within SM_b20409 at 425.305 kb on + strand, within SM_b20409 at 425.306 kb on - strand, within SM_b20409 at 425.307 kb on + strand, within SM_b20409 at 425.308 kb on - strand, within SM_b20409 at 425.308 kb on - strand, within SM_b20409 at 425.351 kb on + strand, within SM_b20409 at 425.577 kb on - strand, within SM_b20409 at 425.643 kb on + strand at 425.643 kb on + strand at 425.643 kb on + strand at 425.643 kb on + strand at 425.644 kb on - strand at 425.644 kb on - strand at 425.711 kb on + strand at 425.712 kb on - strand at 425.712 kb on - strand at 425.712 kb on - strand at 425.712 kb on - strand at 425.712 kb on - strand at 425.720 kb on + strand at 425.720 kb on + strand at 425.720 kb on + strand at 425.721 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 423,522 + SM_b20407 0.63 +0.5 423,522 + SM_b20407 0.63 -2.2 423,522 + SM_b20407 0.63 +1.3 423,522 + SM_b20407 0.63 +0.5 423,523 - SM_b20407 0.63 +1.7 423,523 - SM_b20407 0.63 -2.8 423,523 - SM_b20407 0.63 +0.6 423,523 - SM_b20407 0.63 +0.5 423,591 + SM_b20407 0.66 -1.4 423,591 + SM_b20407 0.66 -0.9 423,591 + SM_b20407 0.66 -3.2 423,591 + SM_b20407 0.66 -1.2 423,592 - SM_b20407 0.66 +1.2 423,592 - SM_b20407 0.66 +0.4 423,701 - SM_b20407 0.71 +0.9 423,840 - SM_b20407 0.77 +1.2 423,882 + SM_b20407 0.79 +0.5 423,882 + SM_b20407 0.79 +0.8 423,915 + SM_b20407 0.81 +0.9 423,915 + SM_b20407 0.81 -1.5 423,916 - SM_b20407 0.81 -1.4 423,916 - SM_b20407 0.81 -0.6 423,916 - SM_b20407 0.81 +1.0 423,916 - SM_b20407 0.81 -3.5 423,916 - SM_b20407 0.81 -1.8 423,916 - SM_b20407 0.81 -1.4 423,916 - SM_b20407 0.81 -0.1 423,916 - SM_b20407 0.81 +0.1 423,960 + SM_b20407 0.83 +0.2 423,960 + SM_b20407 0.83 +1.5 423,962 + SM_b20407 0.83 +0.1 423,963 - SM_b20407 0.83 -0.2 423,963 - SM_b20407 0.83 +0.8 424,360 - -0.9 424,360 - -0.4 424,431 + SM_b20408 0.17 -1.1 424,432 - SM_b20408 0.17 +0.9 424,432 - SM_b20408 0.17 +0.7 424,432 - SM_b20408 0.17 -0.2 424,432 - SM_b20408 0.17 -1.0 424,432 - SM_b20408 0.17 -0.4 424,432 - SM_b20408 0.17 -0.5 424,433 + SM_b20408 0.17 -0.7 424,433 + SM_b20408 0.17 -2.4 424,434 - SM_b20408 0.17 -2.9 424,458 + SM_b20408 0.22 +0.0 424,591 - SM_b20408 0.49 -0.5 424,610 + SM_b20408 0.53 -0.3 424,948 - SM_b20409 0.13 -0.4 424,948 - SM_b20409 0.13 +0.9 425,061 - SM_b20409 0.26 +0.2 425,061 - SM_b20409 0.26 +0.5 425,205 + SM_b20409 0.44 -0.7 425,205 + SM_b20409 0.44 -3.5 425,205 + SM_b20409 0.44 -0.8 425,206 - SM_b20409 0.44 +0.3 425,206 - SM_b20409 0.44 -0.6 425,305 + SM_b20409 0.56 -0.4 425,305 + SM_b20409 0.56 -1.4 425,306 - SM_b20409 0.56 -0.1 425,307 + SM_b20409 0.56 +4.1 425,308 - SM_b20409 0.56 +0.3 425,308 - SM_b20409 0.56 +2.9 425,351 + SM_b20409 0.61 -0.1 425,577 - SM_b20409 0.89 -0.3 425,643 + +0.2 425,643 + -2.4 425,643 + -1.7 425,643 + -0.2 425,644 - -2.9 425,644 - -2.4 425,711 + +4.7 425,712 - -0.6 425,712 - -1.2 425,712 - +1.9 425,712 - -1.2 425,712 - -1.4 425,720 + -2.1 425,720 + -3.2 425,720 + +1.1 425,721 - +0.2
Or see this region's nucleotide sequence