Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20280 and SM_b20281 are separated by 81 nucleotides SM_b20281 and SM_b20282 are separated by 3 nucleotides SM_b20282 and SM_b20283 are separated by 10 nucleotides
SM_b20280: SM_b20280 - hypothetical protein, at 284,069 to 285,535
_b20280
SM_b20281: SM_b20281 - spermidine/putrescine ABC transporter ATP-binding protein, at 285,617 to 286,687
_b20281
SM_b20282: SM_b20282 - spermidine/putrescine ABC transporter permease, at 286,691 to 287,482
_b20282
SM_b20283: SM_b20283 - spermidine/putrescine ABC transporter permease, at 287,493 to 288,476
_b20283
Position (kb) 285
286
287 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 284.662 kb on - strand, within SM_b20280 at 285.196 kb on - strand, within SM_b20280 at 285.471 kb on + strand at 285.471 kb on + strand at 285.472 kb on - strand at 285.539 kb on - strand at 285.829 kb on - strand, within SM_b20281 at 285.829 kb on - strand, within SM_b20281 at 286.020 kb on + strand, within SM_b20281 at 286.020 kb on + strand, within SM_b20281 at 286.020 kb on + strand, within SM_b20281 at 286.434 kb on + strand, within SM_b20281 at 286.435 kb on - strand, within SM_b20281 at 286.504 kb on - strand, within SM_b20281 at 286.504 kb on - strand, within SM_b20281 at 286.504 kb on - strand, within SM_b20281 at 286.555 kb on + strand, within SM_b20281 at 286.883 kb on + strand, within SM_b20282 at 287.231 kb on + strand, within SM_b20282 at 287.278 kb on - strand, within SM_b20282 at 287.415 kb on + strand at 287.415 kb on + strand at 287.416 kb on - strand at 287.434 kb on - strand at 287.473 kb on - strand at 287.494 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 284,662 - SM_b20280 0.40 -2.1 285,196 - SM_b20280 0.77 -3.4 285,471 + -0.9 285,471 + -0.1 285,472 - -1.3 285,539 - -1.1 285,829 - SM_b20281 0.20 -1.1 285,829 - SM_b20281 0.20 +0.9 286,020 + SM_b20281 0.38 -1.0 286,020 + SM_b20281 0.38 -0.5 286,020 + SM_b20281 0.38 -3.5 286,434 + SM_b20281 0.76 +0.9 286,435 - SM_b20281 0.76 -0.8 286,504 - SM_b20281 0.83 -3.7 286,504 - SM_b20281 0.83 -1.4 286,504 - SM_b20281 0.83 +1.9 286,555 + SM_b20281 0.88 +0.9 286,883 + SM_b20282 0.24 +0.7 287,231 + SM_b20282 0.68 +1.2 287,278 - SM_b20282 0.74 +2.7 287,415 + +0.9 287,415 + -0.4 287,416 - +1.1 287,434 - -1.2 287,473 - +0.6 287,494 + -0.8
Or see this region's nucleotide sequence