Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20257 and SM_b20258 are separated by 104 nucleotides SM_b20258 and SM_b20259 are separated by 120 nucleotides SM_b20259 and SM_b20260 are separated by 47 nucleotides SM_b20260 and SM_b20261 are separated by 63 nucleotides
SM_b20257: SM_b20257 - adenylate cyclase, at 262,074 to 263,132
_b20257
SM_b20258: SM_b20258 - transcriptional regulator, at 263,237 to 263,947
_b20258
SM_b20259: SM_b20259 - dihydrodipicolinate synthase, at 264,068 to 265,030
_b20259
SM_b20260: SM_b20260 - hypothetical protein, at 265,078 to 265,470
_b20260
SM_b20261: SM_b20261 - malate dehydrogenase, at 265,534 to 266,571
_b20261
Position (kb) 264
265
266 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 263.114 kb on + strand at 263.306 kb on + strand at 263.306 kb on + strand at 263.534 kb on + strand, within SM_b20258 at 263.713 kb on - strand, within SM_b20258 at 263.713 kb on - strand, within SM_b20258 at 263.762 kb on + strand, within SM_b20258 at 263.775 kb on - strand, within SM_b20258 at 263.775 kb on - strand, within SM_b20258 at 263.775 kb on - strand, within SM_b20258 at 264.200 kb on + strand, within SM_b20259 at 264.270 kb on + strand, within SM_b20259 at 264.271 kb on - strand, within SM_b20259 at 264.376 kb on - strand, within SM_b20259 at 264.376 kb on - strand, within SM_b20259 at 264.439 kb on - strand, within SM_b20259 at 264.439 kb on - strand, within SM_b20259 at 264.473 kb on + strand, within SM_b20259 at 264.474 kb on - strand, within SM_b20259 at 264.474 kb on - strand, within SM_b20259 at 264.485 kb on + strand, within SM_b20259 at 264.486 kb on - strand, within SM_b20259 at 264.486 kb on - strand, within SM_b20259 at 264.486 kb on - strand, within SM_b20259 at 264.488 kb on + strand, within SM_b20259 at 264.711 kb on + strand, within SM_b20259 at 264.854 kb on + strand, within SM_b20259 at 264.980 kb on + strand at 264.980 kb on + strand at 265.020 kb on - strand at 265.142 kb on - strand, within SM_b20260 at 265.290 kb on - strand, within SM_b20260 at 265.340 kb on + strand, within SM_b20260 at 265.341 kb on - strand, within SM_b20260 at 265.341 kb on - strand, within SM_b20260 at 265.799 kb on + strand, within SM_b20261 at 265.800 kb on - strand, within SM_b20261 at 265.800 kb on - strand, within SM_b20261 at 265.800 kb on - strand, within SM_b20261
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 263,114 + -3.5 263,306 + -2.1 263,306 + -2.1 263,534 + SM_b20258 0.42 -1.2 263,713 - SM_b20258 0.67 -1.6 263,713 - SM_b20258 0.67 -1.5 263,762 + SM_b20258 0.74 -1.2 263,775 - SM_b20258 0.76 +0.9 263,775 - SM_b20258 0.76 +3.8 263,775 - SM_b20258 0.76 -1.4 264,200 + SM_b20259 0.14 -0.0 264,270 + SM_b20259 0.21 -0.6 264,271 - SM_b20259 0.21 -0.4 264,376 - SM_b20259 0.32 +0.7 264,376 - SM_b20259 0.32 -1.9 264,439 - SM_b20259 0.39 +3.7 264,439 - SM_b20259 0.39 +1.2 264,473 + SM_b20259 0.42 +0.7 264,474 - SM_b20259 0.42 +0.6 264,474 - SM_b20259 0.42 +1.8 264,485 + SM_b20259 0.43 -0.6 264,486 - SM_b20259 0.43 +1.4 264,486 - SM_b20259 0.43 -0.1 264,486 - SM_b20259 0.43 -1.3 264,488 + SM_b20259 0.44 -1.1 264,711 + SM_b20259 0.67 +0.4 264,854 + SM_b20259 0.82 +1.3 264,980 + +3.5 264,980 + +0.8 265,020 - +2.0 265,142 - SM_b20260 0.16 +1.6 265,290 - SM_b20260 0.54 +0.9 265,340 + SM_b20260 0.67 +1.9 265,341 - SM_b20260 0.67 +0.8 265,341 - SM_b20260 0.67 -2.4 265,799 + SM_b20261 0.26 +0.2 265,800 - SM_b20261 0.26 -3.8 265,800 - SM_b20261 0.26 -1.4 265,800 - SM_b20261 0.26 -3.1
Or see this region's nucleotide sequence