Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20255 and SM_b20256 are separated by 131 nucleotides SM_b20256 and SM_b20257 are separated by 182 nucleotides SM_b20257 and SM_b20258 are separated by 104 nucleotides SM_b20258 and SM_b20259 are separated by 120 nucleotides
SM_b20255: SM_b20255 - hypothetical protein, at 260,759 to 261,310
_b20255
SM_b20256: SM_b20256 - hypothetical protein, at 261,442 to 261,891
_b20256
SM_b20257: SM_b20257 - adenylate cyclase, at 262,074 to 263,132
_b20257
SM_b20258: SM_b20258 - transcriptional regulator, at 263,237 to 263,947
_b20258
SM_b20259: SM_b20259 - dihydrodipicolinate synthase, at 264,068 to 265,030
_b20259
Position (kb) 262
263
264 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 261.355 kb on + strand at 261.674 kb on + strand, within SM_b20256 at 261.674 kb on + strand, within SM_b20256 at 261.736 kb on + strand, within SM_b20256 at 261.749 kb on + strand, within SM_b20256 at 261.964 kb on + strand at 261.965 kb on - strand at 262.062 kb on + strand at 262.063 kb on - strand at 262.063 kb on - strand at 262.600 kb on - strand, within SM_b20257 at 262.699 kb on + strand, within SM_b20257 at 262.700 kb on - strand, within SM_b20257 at 262.807 kb on + strand, within SM_b20257 at 262.807 kb on + strand, within SM_b20257 at 262.808 kb on - strand, within SM_b20257 at 263.114 kb on + strand at 263.306 kb on + strand at 263.306 kb on + strand at 263.534 kb on + strand, within SM_b20258 at 263.713 kb on - strand, within SM_b20258 at 263.713 kb on - strand, within SM_b20258 at 263.762 kb on + strand, within SM_b20258 at 263.775 kb on - strand, within SM_b20258 at 263.775 kb on - strand, within SM_b20258 at 263.775 kb on - strand, within SM_b20258
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 261,355 + -1.0 261,674 + SM_b20256 0.52 +0.9 261,674 + SM_b20256 0.52 +1.7 261,736 + SM_b20256 0.65 -0.3 261,749 + SM_b20256 0.68 +1.4 261,964 + +0.9 261,965 - -0.2 262,062 + -2.2 262,063 - +0.9 262,063 - +0.6 262,600 - SM_b20257 0.50 -0.4 262,699 + SM_b20257 0.59 -1.2 262,700 - SM_b20257 0.59 +0.2 262,807 + SM_b20257 0.69 +0.5 262,807 + SM_b20257 0.69 +1.4 262,808 - SM_b20257 0.69 -2.9 263,114 + -3.5 263,306 + -2.1 263,306 + -2.1 263,534 + SM_b20258 0.42 -1.2 263,713 - SM_b20258 0.67 -1.6 263,713 - SM_b20258 0.67 -1.5 263,762 + SM_b20258 0.74 -1.2 263,775 - SM_b20258 0.76 +0.9 263,775 - SM_b20258 0.76 +3.8 263,775 - SM_b20258 0.76 -1.4
Or see this region's nucleotide sequence