Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20215 and SM_b20216 are separated by 140 nucleotides SM_b20216 and SM_b20217 are separated by 217 nucleotides
SM_b20215: SM_b20215 - transcriptional regulator, at 222,993 to 223,973
_b20215
SM_b20216: SM_b20216 - epoxide hydrolase, at 224,114 to 225,160
_b20216
SM_b20217: SM_b20217 - hypothetical protein, at 225,378 to 226,043
_b20217
Position (kb) 224
225
226 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 223.129 kb on - strand, within SM_b20215 at 223.135 kb on + strand, within SM_b20215 at 223.136 kb on - strand, within SM_b20215 at 223.193 kb on + strand, within SM_b20215 at 223.194 kb on - strand, within SM_b20215 at 223.218 kb on + strand, within SM_b20215 at 223.430 kb on + strand, within SM_b20215 at 223.534 kb on - strand, within SM_b20215 at 223.666 kb on - strand, within SM_b20215 at 223.994 kb on + strand at 223.999 kb on + strand at 223.999 kb on + strand at 224.000 kb on - strand at 224.023 kb on + strand at 224.066 kb on + strand at 224.066 kb on + strand at 224.067 kb on - strand at 224.260 kb on + strand, within SM_b20216 at 224.260 kb on + strand, within SM_b20216 at 224.277 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.308 kb on - strand, within SM_b20216 at 224.384 kb on + strand, within SM_b20216 at 224.385 kb on - strand, within SM_b20216 at 224.596 kb on + strand, within SM_b20216 at 224.651 kb on + strand, within SM_b20216 at 224.757 kb on - strand, within SM_b20216 at 224.933 kb on + strand, within SM_b20216 at 225.259 kb on + strand at 225.259 kb on + strand at 225.277 kb on + strand at 225.277 kb on + strand at 225.277 kb on + strand at 225.277 kb on + strand at 225.277 kb on + strand at 225.278 kb on - strand at 225.419 kb on + strand at 225.419 kb on + strand at 225.435 kb on - strand at 225.545 kb on + strand, within SM_b20217 at 225.624 kb on + strand, within SM_b20217 at 225.694 kb on + strand, within SM_b20217 at 225.735 kb on + strand, within SM_b20217 at 225.736 kb on - strand, within SM_b20217 at 225.787 kb on - strand, within SM_b20217 at 225.796 kb on - strand, within SM_b20217 at 225.796 kb on - strand, within SM_b20217 at 226.002 kb on + strand at 226.142 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 223,129 - SM_b20215 0.14 +0.2 223,135 + SM_b20215 0.14 +1.0 223,136 - SM_b20215 0.15 +0.9 223,193 + SM_b20215 0.20 -0.3 223,194 - SM_b20215 0.20 +0.3 223,218 + SM_b20215 0.23 -1.8 223,430 + SM_b20215 0.45 +0.2 223,534 - SM_b20215 0.55 +1.0 223,666 - SM_b20215 0.69 +0.1 223,994 + +0.5 223,999 + -1.4 223,999 + -3.6 224,000 - +0.6 224,023 + +0.3 224,066 + +0.2 224,066 + -3.2 224,067 - +1.4 224,260 + SM_b20216 0.14 +1.2 224,260 + SM_b20216 0.14 -3.1 224,277 + SM_b20216 0.16 +0.7 224,307 + SM_b20216 0.18 +0.6 224,307 + SM_b20216 0.18 +1.6 224,307 + SM_b20216 0.18 +0.8 224,307 + SM_b20216 0.18 -2.0 224,308 - SM_b20216 0.19 +1.5 224,384 + SM_b20216 0.26 -2.1 224,385 - SM_b20216 0.26 -0.1 224,596 + SM_b20216 0.46 -0.3 224,651 + SM_b20216 0.51 -1.0 224,757 - SM_b20216 0.61 +1.4 224,933 + SM_b20216 0.78 +1.4 225,259 + +0.1 225,259 + +1.0 225,277 + -2.5 225,277 + -2.7 225,277 + -1.2 225,277 + -1.5 225,277 + -2.6 225,278 - +1.9 225,419 + +0.4 225,419 + -0.1 225,435 - +0.8 225,545 + SM_b20217 0.25 +2.7 225,624 + SM_b20217 0.37 +0.1 225,694 + SM_b20217 0.47 +0.7 225,735 + SM_b20217 0.54 -1.0 225,736 - SM_b20217 0.54 +0.6 225,787 - SM_b20217 0.61 +0.2 225,796 - SM_b20217 0.63 -2.2 225,796 - SM_b20217 0.63 -0.8 226,002 + +0.3 226,142 - -2.1
Or see this region's nucleotide sequence