Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20186 and SM_b20194 are separated by 311 nucleotides SM_b20194 and SM_b20195 are separated by 3 nucleotides SM_b20195 and SM_b20196 overlap by 4 nucleotides
SM_b20186: SM_b20186 - glutathione-dependent formaldehyde-activating protein, at 202,255 to 202,824
_b20186
SM_b20194: SM_b20194 - hypothetical protein, at 203,136 to 203,702
_b20194
SM_b20195: SM_b20195 - pentose-5-phosphate-3-epimerase, at 203,706 to 204,392
_b20195
SM_b20196: SM_b20196 - CbbX protein, at 204,389 to 205,324
_b20196
Position (kb) 203
204 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 202.146 kb on - strand at 202.146 kb on - strand at 202.185 kb on + strand at 202.265 kb on + strand at 202.558 kb on + strand, within SM_b20186 at 202.559 kb on - strand, within SM_b20186 at 202.559 kb on - strand, within SM_b20186 at 202.559 kb on - strand, within SM_b20186 at 202.559 kb on - strand, within SM_b20186 at 202.559 kb on - strand, within SM_b20186 at 202.592 kb on - strand, within SM_b20186 at 202.712 kb on + strand, within SM_b20186 at 202.975 kb on + strand at 203.056 kb on + strand at 203.057 kb on - strand at 203.178 kb on + strand at 203.239 kb on + strand, within SM_b20194 at 203.395 kb on + strand, within SM_b20194 at 203.395 kb on + strand, within SM_b20194 at 203.458 kb on + strand, within SM_b20194 at 203.557 kb on + strand, within SM_b20194 at 203.576 kb on + strand, within SM_b20194 at 203.576 kb on + strand, within SM_b20194 at 203.617 kb on - strand, within SM_b20194 at 203.693 kb on + strand at 203.767 kb on + strand at 203.768 kb on - strand at 203.877 kb on + strand, within SM_b20195 at 204.011 kb on - strand, within SM_b20195 at 204.011 kb on - strand, within SM_b20195 at 204.011 kb on - strand, within SM_b20195 at 204.163 kb on + strand, within SM_b20195 at 204.291 kb on + strand, within SM_b20195 at 204.291 kb on + strand, within SM_b20195 at 204.292 kb on - strand, within SM_b20195 at 204.603 kb on + strand, within SM_b20196 at 204.603 kb on + strand, within SM_b20196 at 204.603 kb on + strand, within SM_b20196 at 204.692 kb on - strand, within SM_b20196
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 202,146 - +0.7 202,146 - -0.4 202,185 + +0.1 202,265 + -2.5 202,558 + SM_b20186 0.53 +1.5 202,559 - SM_b20186 0.53 +1.4 202,559 - SM_b20186 0.53 -1.9 202,559 - SM_b20186 0.53 -0.6 202,559 - SM_b20186 0.53 -3.5 202,559 - SM_b20186 0.53 -0.3 202,592 - SM_b20186 0.59 -1.2 202,712 + SM_b20186 0.80 +0.2 202,975 + +1.0 203,056 + -2.0 203,057 - -0.0 203,178 + -0.3 203,239 + SM_b20194 0.18 +0.4 203,395 + SM_b20194 0.46 -1.1 203,395 + SM_b20194 0.46 -0.6 203,458 + SM_b20194 0.57 +0.7 203,557 + SM_b20194 0.74 -2.1 203,576 + SM_b20194 0.78 -0.7 203,576 + SM_b20194 0.78 -1.2 203,617 - SM_b20194 0.85 -0.6 203,693 + +1.0 203,767 + -2.4 203,768 - +0.0 203,877 + SM_b20195 0.25 -1.3 204,011 - SM_b20195 0.44 +0.7 204,011 - SM_b20195 0.44 +0.2 204,011 - SM_b20195 0.44 -1.0 204,163 + SM_b20195 0.67 -0.8 204,291 + SM_b20195 0.85 -0.3 204,291 + SM_b20195 0.85 +1.7 204,292 - SM_b20195 0.85 +0.4 204,603 + SM_b20196 0.23 -3.8 204,603 + SM_b20196 0.23 +0.2 204,603 + SM_b20196 0.23 +1.7 204,692 - SM_b20196 0.32 -0.1
Or see this region's nucleotide sequence