Strain Fitness in Sinorhizobium meliloti 1021 around SM_b20175

Experiment: Polymyxin B sulfate 0.00005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSM_b20173 and SM_b20174 are separated by 78 nucleotidesSM_b20174 and SM_b20175 overlap by 44 nucleotidesSM_b20175 and SM_b20176 overlap by 4 nucleotidesSM_b20176 and SM_b20177 are separated by 173 nucleotides SM_b20173: SM_b20173 - methanol dehydrogenase, large subunit, at 190,974 to 192,779 _b20173 SM_b20174: SM_b20174 - cytochrome c protein, at 192,858 to 193,388 _b20174 SM_b20175: SM_b20175 - hypothetical protein, at 193,345 to 194,211 _b20175 SM_b20176: SM_b20176 - hypothetical protein, at 194,208 to 194,753 _b20176 SM_b20177: SM_b20177 - hypothetical protein, at 194,927 to 195,448 _b20177 Position (kb) 193 194 195Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 192.597 kb on + strand, within SM_b20173at 192.597 kb on + strand, within SM_b20173at 192.598 kb on - strand, within SM_b20173at 192.598 kb on - strand, within SM_b20173at 192.598 kb on - strand, within SM_b20173at 192.618 kb on + strandat 192.618 kb on + strandat 192.715 kb on - strandat 192.732 kb on + strandat 192.732 kb on + strandat 192.732 kb on + strandat 192.733 kb on - strandat 192.778 kb on - strandat 192.816 kb on - strandat 192.834 kb on + strandat 192.834 kb on + strandat 192.908 kb on + strandat 192.908 kb on + strandat 192.981 kb on + strand, within SM_b20174at 192.981 kb on + strand, within SM_b20174at 192.984 kb on + strand, within SM_b20174at 192.984 kb on + strand, within SM_b20174at 192.985 kb on - strand, within SM_b20174at 192.985 kb on - strand, within SM_b20174at 193.065 kb on + strand, within SM_b20174at 193.116 kb on + strand, within SM_b20174at 193.116 kb on + strand, within SM_b20174at 193.117 kb on - strand, within SM_b20174at 193.155 kb on + strand, within SM_b20174at 193.156 kb on - strand, within SM_b20174at 193.159 kb on - strand, within SM_b20174at 193.257 kb on + strand, within SM_b20174at 193.258 kb on - strand, within SM_b20174at 193.272 kb on + strand, within SM_b20174at 193.272 kb on + strand, within SM_b20174at 193.272 kb on + strand, within SM_b20174at 193.272 kb on + strand, within SM_b20174at 193.272 kb on + strand, within SM_b20174at 193.272 kb on + strand, within SM_b20174at 193.272 kb on + strand, within SM_b20174at 193.272 kb on + strand, within SM_b20174at 193.273 kb on - strand, within SM_b20174at 193.273 kb on - strand, within SM_b20174at 193.273 kb on - strand, within SM_b20174at 193.273 kb on - strand, within SM_b20174at 193.273 kb on - strand, within SM_b20174at 193.273 kb on - strand, within SM_b20174at 193.273 kb on - strand, within SM_b20174at 193.386 kb on + strandat 193.386 kb on + strandat 193.461 kb on - strand, within SM_b20175at 193.588 kb on + strand, within SM_b20175at 193.589 kb on - strand, within SM_b20175at 193.702 kb on + strand, within SM_b20175at 193.703 kb on - strand, within SM_b20175at 193.705 kb on + strand, within SM_b20175at 193.705 kb on + strand, within SM_b20175at 193.706 kb on - strand, within SM_b20175at 193.706 kb on - strand, within SM_b20175at 193.706 kb on - strand, within SM_b20175at 193.706 kb on - strand, within SM_b20175at 193.708 kb on - strand, within SM_b20175at 193.708 kb on - strand, within SM_b20175at 193.708 kb on - strand, within SM_b20175at 193.708 kb on - strand, within SM_b20175at 193.708 kb on - strand, within SM_b20175at 193.708 kb on - strand, within SM_b20175at 193.708 kb on - strand, within SM_b20175at 193.717 kb on + strand, within SM_b20175at 193.718 kb on - strand, within SM_b20175at 193.718 kb on - strand, within SM_b20175at 193.718 kb on - strand, within SM_b20175at 193.718 kb on - strand, within SM_b20175at 193.830 kb on + strand, within SM_b20175at 193.831 kb on - strand, within SM_b20175at 193.867 kb on + strand, within SM_b20175at 193.868 kb on - strand, within SM_b20175at 194.050 kb on + strand, within SM_b20175at 194.407 kb on + strand, within SM_b20176at 194.488 kb on + strand, within SM_b20176at 194.488 kb on + strand, within SM_b20176at 194.539 kb on - strand, within SM_b20176at 194.652 kb on + strand, within SM_b20176at 194.658 kb on + strand, within SM_b20176at 194.658 kb on + strand, within SM_b20176at 194.659 kb on - strand, within SM_b20176at 194.659 kb on - strand, within SM_b20176at 194.822 kb on - strandat 194.959 kb on - strandat 194.959 kb on - strandat 195.065 kb on + strand, within SM_b20177at 195.070 kb on + strand, within SM_b20177at 195.071 kb on - strand, within SM_b20177at 195.108 kb on + strand, within SM_b20177at 195.108 kb on + strand, within SM_b20177

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml
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192,597 + SM_b20173 0.90 +0.2
192,597 + SM_b20173 0.90 +0.6
192,598 - SM_b20173 0.90 -2.6
192,598 - SM_b20173 0.90 -0.4
192,598 - SM_b20173 0.90 +1.0
192,618 + +0.8
192,618 + +0.6
192,715 - +0.0
192,732 + -0.5
192,732 + +0.1
192,732 + -3.1
192,733 - +0.6
192,778 - -1.5
192,816 - -1.0
192,834 + -0.3
192,834 + -0.2
192,908 + +0.2
192,908 + -2.3
192,981 + SM_b20174 0.23 -0.2
192,981 + SM_b20174 0.23 -2.8
192,984 + SM_b20174 0.24 -1.1
192,984 + SM_b20174 0.24 +2.9
192,985 - SM_b20174 0.24 +3.0
192,985 - SM_b20174 0.24 +1.3
193,065 + SM_b20174 0.39 +0.0
193,116 + SM_b20174 0.49 -1.1
193,116 + SM_b20174 0.49 +0.1
193,117 - SM_b20174 0.49 -1.5
193,155 + SM_b20174 0.56 -2.3
193,156 - SM_b20174 0.56 -0.9
193,159 - SM_b20174 0.57 +0.8
193,257 + SM_b20174 0.75 -0.1
193,258 - SM_b20174 0.75 -1.8
193,272 + SM_b20174 0.78 +0.3
193,272 + SM_b20174 0.78 +0.3
193,272 + SM_b20174 0.78 -0.7
193,272 + SM_b20174 0.78 -0.7
193,272 + SM_b20174 0.78 +0.0
193,272 + SM_b20174 0.78 +0.5
193,272 + SM_b20174 0.78 +1.1
193,272 + SM_b20174 0.78 +0.1
193,273 - SM_b20174 0.78 -1.0
193,273 - SM_b20174 0.78 -1.6
193,273 - SM_b20174 0.78 +1.0
193,273 - SM_b20174 0.78 +0.9
193,273 - SM_b20174 0.78 +0.8
193,273 - SM_b20174 0.78 +0.1
193,273 - SM_b20174 0.78 +0.3
193,386 + +0.4
193,386 + +0.1
193,461 - SM_b20175 0.13 +1.5
193,588 + SM_b20175 0.28 +1.2
193,589 - SM_b20175 0.28 +0.6
193,702 + SM_b20175 0.41 +0.2
193,703 - SM_b20175 0.41 +2.6
193,705 + SM_b20175 0.42 +0.4
193,705 + SM_b20175 0.42 +0.4
193,706 - SM_b20175 0.42 -0.5
193,706 - SM_b20175 0.42 +2.2
193,706 - SM_b20175 0.42 +0.5
193,706 - SM_b20175 0.42 +2.7
193,708 - SM_b20175 0.42 -1.6
193,708 - SM_b20175 0.42 -0.5
193,708 - SM_b20175 0.42 -2.0
193,708 - SM_b20175 0.42 +0.9
193,708 - SM_b20175 0.42 +0.2
193,708 - SM_b20175 0.42 -4.1
193,708 - SM_b20175 0.42 +0.4
193,717 + SM_b20175 0.43 +0.0
193,718 - SM_b20175 0.43 -1.6
193,718 - SM_b20175 0.43 -1.0
193,718 - SM_b20175 0.43 -0.8
193,718 - SM_b20175 0.43 +0.0
193,830 + SM_b20175 0.56 +0.7
193,831 - SM_b20175 0.56 +0.7
193,867 + SM_b20175 0.60 +0.7
193,868 - SM_b20175 0.60 +0.6
194,050 + SM_b20175 0.81 -0.4
194,407 + SM_b20176 0.36 -3.1
194,488 + SM_b20176 0.51 +1.9
194,488 + SM_b20176 0.51 -3.2
194,539 - SM_b20176 0.61 -0.6
194,652 + SM_b20176 0.81 +0.7
194,658 + SM_b20176 0.82 -0.9
194,658 + SM_b20176 0.82 -2.2
194,659 - SM_b20176 0.83 +0.0
194,659 - SM_b20176 0.83 +2.2
194,822 - +2.7
194,959 - -0.4
194,959 - -0.3
195,065 + SM_b20177 0.26 -0.0
195,070 + SM_b20177 0.27 -2.2
195,071 - SM_b20177 0.28 -1.6
195,108 + SM_b20177 0.35 -0.4
195,108 + SM_b20177 0.35 -0.3

Or see this region's nucleotide sequence