Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20170 and SM_b20171 overlap by 4 nucleotides SM_b20171 and SM_b20172 are separated by 13 nucleotides SM_b20172 and SM_b20173 are separated by 177 nucleotides
SM_b20170: SM_b20170 - glutathione-dependent formaldehyde dehydrogenase, at 188,406 to 189,533
_b20170
SM_b20171: SM_b20171 - carboxylesterase, at 189,530 to 190,372
_b20171
SM_b20172: SM_b20172 - cytochrome c protein, at 190,386 to 190,796
_b20172
SM_b20173: SM_b20173 - methanol dehydrogenase, large subunit, at 190,974 to 192,779
_b20173
Position (kb) 190
191 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 189.528 kb on + strand at 189.528 kb on + strand at 189.552 kb on + strand at 189.638 kb on + strand, within SM_b20171 at 189.639 kb on - strand, within SM_b20171 at 189.684 kb on - strand, within SM_b20171 at 189.822 kb on - strand, within SM_b20171 at 189.848 kb on + strand, within SM_b20171 at 189.849 kb on - strand, within SM_b20171 at 189.884 kb on + strand, within SM_b20171 at 189.893 kb on + strand at 189.893 kb on + strand, within SM_b20171 at 189.893 kb on + strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.899 kb on + strand, within SM_b20171 at 189.899 kb on + strand, within SM_b20171 at 189.900 kb on - strand, within SM_b20171 at 189.900 kb on - strand, within SM_b20171 at 189.900 kb on - strand, within SM_b20171 at 189.900 kb on - strand, within SM_b20171 at 189.902 kb on - strand, within SM_b20171 at 190.133 kb on + strand, within SM_b20171 at 190.133 kb on + strand, within SM_b20171 at 190.133 kb on + strand, within SM_b20171 at 190.178 kb on - strand, within SM_b20171 at 190.304 kb on + strand at 190.307 kb on + strand at 190.307 kb on + strand at 190.615 kb on - strand, within SM_b20172 at 190.615 kb on - strand, within SM_b20172 at 190.620 kb on + strand, within SM_b20172 at 190.621 kb on - strand, within SM_b20172 at 190.621 kb on - strand, within SM_b20172 at 190.658 kb on + strand, within SM_b20172 at 190.658 kb on + strand, within SM_b20172 at 190.768 kb on + strand at 190.769 kb on - strand at 190.769 kb on - strand at 190.776 kb on + strand at 190.836 kb on - strand at 190.836 kb on - strand at 190.836 kb on - strand at 190.843 kb on + strand at 190.988 kb on + strand at 190.988 kb on + strand at 190.989 kb on - strand at 190.989 kb on - strand at 191.003 kb on + strand at 191.003 kb on + strand at 191.100 kb on + strand at 191.235 kb on + strand, within SM_b20173 at 191.235 kb on + strand, within SM_b20173 at 191.235 kb on + strand, within SM_b20173 at 191.236 kb on - strand, within SM_b20173 at 191.236 kb on - strand, within SM_b20173 at 191.243 kb on + strand, within SM_b20173 at 191.265 kb on + strand, within SM_b20173 at 191.308 kb on - strand, within SM_b20173 at 191.477 kb on + strand, within SM_b20173 at 191.477 kb on + strand, within SM_b20173 at 191.616 kb on + strand, within SM_b20173 at 191.616 kb on + strand, within SM_b20173 at 191.617 kb on - strand, within SM_b20173 at 191.640 kb on - strand, within SM_b20173 at 191.758 kb on - strand, within SM_b20173 at 191.764 kb on - strand, within SM_b20173 at 191.785 kb on + strand, within SM_b20173 at 191.787 kb on + strand, within SM_b20173 at 191.788 kb on - strand at 191.790 kb on + strand, within SM_b20173 at 191.790 kb on + strand, within SM_b20173
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 189,528 + -0.1 189,528 + -0.7 189,552 + -1.2 189,638 + SM_b20171 0.13 +0.7 189,639 - SM_b20171 0.13 -0.2 189,684 - SM_b20171 0.18 +0.9 189,822 - SM_b20171 0.35 +1.0 189,848 + SM_b20171 0.38 -0.1 189,849 - SM_b20171 0.38 +0.3 189,884 + SM_b20171 0.42 +0.2 189,893 + -1.0 189,893 + SM_b20171 0.43 +1.1 189,893 + SM_b20171 0.43 -0.1 189,894 - SM_b20171 0.43 +0.6 189,894 - SM_b20171 0.43 -1.1 189,894 - SM_b20171 0.43 +0.2 189,894 - SM_b20171 0.43 -0.3 189,894 - SM_b20171 0.43 -1.9 189,894 - SM_b20171 0.43 +0.2 189,894 - SM_b20171 0.43 +1.1 189,899 + SM_b20171 0.44 -1.1 189,899 + SM_b20171 0.44 -1.4 189,900 - SM_b20171 0.44 -2.4 189,900 - SM_b20171 0.44 -0.6 189,900 - SM_b20171 0.44 +1.6 189,900 - SM_b20171 0.44 +0.6 189,902 - SM_b20171 0.44 +1.2 190,133 + SM_b20171 0.72 +2.4 190,133 + SM_b20171 0.72 +0.6 190,133 + SM_b20171 0.72 -2.9 190,178 - SM_b20171 0.77 +0.2 190,304 + +2.6 190,307 + -1.6 190,307 + -0.1 190,615 - SM_b20172 0.56 +1.0 190,615 - SM_b20172 0.56 -2.9 190,620 + SM_b20172 0.57 -0.5 190,621 - SM_b20172 0.57 -2.2 190,621 - SM_b20172 0.57 +0.4 190,658 + SM_b20172 0.66 +0.0 190,658 + SM_b20172 0.66 -3.2 190,768 + -0.1 190,769 - +1.1 190,769 - +2.6 190,776 + +0.7 190,836 - -0.1 190,836 - +0.5 190,836 - +1.4 190,843 + -0.1 190,988 + +0.0 190,988 + +0.2 190,989 - +0.2 190,989 - +1.9 191,003 + +1.1 191,003 + +0.4 191,100 + -0.5 191,235 + SM_b20173 0.14 -3.2 191,235 + SM_b20173 0.14 +0.1 191,235 + SM_b20173 0.14 -0.4 191,236 - SM_b20173 0.15 -0.6 191,236 - SM_b20173 0.15 -1.3 191,243 + SM_b20173 0.15 -0.6 191,265 + SM_b20173 0.16 +1.0 191,308 - SM_b20173 0.18 +1.0 191,477 + SM_b20173 0.28 -2.2 191,477 + SM_b20173 0.28 -0.3 191,616 + SM_b20173 0.36 -4.2 191,616 + SM_b20173 0.36 -3.1 191,617 - SM_b20173 0.36 +0.8 191,640 - SM_b20173 0.37 +0.6 191,758 - SM_b20173 0.43 -0.9 191,764 - SM_b20173 0.44 -0.2 191,785 + SM_b20173 0.45 +0.6 191,787 + SM_b20173 0.45 +0.3 191,788 - +1.0 191,790 + SM_b20173 0.45 -2.2 191,790 + SM_b20173 0.45 +0.5
Or see this region's nucleotide sequence