Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20128 and SM_b20129 are separated by 449 nucleotides SM_b20129 and SM_b20130 are separated by 50 nucleotides SM_b20130 and SM_b20131 overlap by 4 nucleotides
SM_b20128: SM_b20128 - deaminase, at 140,042 to 141,355
_b20128
SM_b20129: SM_b20129 - transcriptional regulator, at 141,805 to 142,584
_b20129
SM_b20130: SM_b20130 - dehydrogenase, at 142,635 to 143,498
_b20130
SM_b20131: SM_b20131 - oxidoreductase, at 143,495 to 143,980
_b20131
Position (kb) 141
142
143 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 141.030 kb on + strand, within SM_b20128 at 141.031 kb on - strand, within SM_b20128 at 141.331 kb on + strand at 141.331 kb on + strand at 141.401 kb on + strand at 141.401 kb on + strand at 141.402 kb on - strand at 141.402 kb on - strand at 141.402 kb on - strand at 141.438 kb on + strand at 141.838 kb on + strand at 141.995 kb on + strand, within SM_b20129 at 141.995 kb on + strand, within SM_b20129 at 142.097 kb on + strand, within SM_b20129 at 142.097 kb on + strand, within SM_b20129 at 142.098 kb on - strand, within SM_b20129 at 142.210 kb on + strand, within SM_b20129 at 142.211 kb on - strand, within SM_b20129 at 142.447 kb on - strand, within SM_b20129 at 142.651 kb on - strand at 142.651 kb on - strand at 142.653 kb on + strand at 142.653 kb on + strand at 142.653 kb on + strand at 142.818 kb on - strand, within SM_b20130 at 142.821 kb on + strand, within SM_b20130 at 142.821 kb on + strand, within SM_b20130 at 142.821 kb on + strand, within SM_b20130 at 142.942 kb on + strand, within SM_b20130 at 142.943 kb on - strand, within SM_b20130 at 143.046 kb on + strand, within SM_b20130 at 143.140 kb on + strand, within SM_b20130 at 143.578 kb on - strand, within SM_b20131
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 141,030 + SM_b20128 0.75 -0.6 141,031 - SM_b20128 0.75 +0.1 141,331 + +0.4 141,331 + +1.6 141,401 + -0.1 141,401 + +0.9 141,402 - +2.0 141,402 - -2.0 141,402 - +1.0 141,438 + +0.8 141,838 + +0.0 141,995 + SM_b20129 0.24 -2.0 141,995 + SM_b20129 0.24 +0.7 142,097 + SM_b20129 0.37 +0.5 142,097 + SM_b20129 0.37 +1.5 142,098 - SM_b20129 0.38 -0.6 142,210 + SM_b20129 0.52 -2.2 142,211 - SM_b20129 0.52 -0.3 142,447 - SM_b20129 0.82 +1.2 142,651 - -3.4 142,651 - -1.2 142,653 + +3.3 142,653 + +0.8 142,653 + -1.1 142,818 - SM_b20130 0.21 +1.9 142,821 + SM_b20130 0.22 +0.0 142,821 + SM_b20130 0.22 +0.5 142,821 + SM_b20130 0.22 -1.3 142,942 + SM_b20130 0.36 +1.0 142,943 - SM_b20130 0.36 -0.4 143,046 + SM_b20130 0.48 +2.3 143,140 + SM_b20130 0.58 -0.9 143,578 - SM_b20131 0.17 +1.0
Or see this region's nucleotide sequence