Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20119 and SM_b22004 overlap by 20 nucleotides SM_b22004 and SM_b20121 overlap by 56 nucleotides SM_b20121 and SM_b20122 are separated by 181 nucleotides SM_b20122 and SM_b20123 are separated by 185 nucleotides
SM_b20119: SM_b20119 - hypothetical protein, at 132,609 to 132,965
_b20119
SM_b22004: SM_b22004 - plasmid stabilization system protein, at 132,946 to 133,338
_b22004
SM_b20121: SM_b20121 - hypothetical protein, at 133,283 to 133,558
_b20121
SM_b20122: SM_b20122 - hypothetical protein, at 133,740 to 134,075
_b20122
SM_b20123: SM_b20123 - transcriptional regulator, at 134,261 to 135,157
_b20123
Position (kb) 133
134
135 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 132.848 kb on + strand, within SM_b20119 at 133.022 kb on + strand, within SM_b22004 at 133.025 kb on + strand, within SM_b22004 at 133.166 kb on + strand, within SM_b22004 at 133.167 kb on - strand, within SM_b22004 at 133.188 kb on - strand, within SM_b22004 at 133.368 kb on - strand, within SM_b20121 at 133.476 kb on + strand, within SM_b20121 at 133.609 kb on - strand at 133.610 kb on + strand at 133.654 kb on + strand at 133.654 kb on + strand at 133.654 kb on + strand at 133.689 kb on + strand at 133.689 kb on + strand at 133.702 kb on + strand at 133.723 kb on - strand at 133.723 kb on - strand at 133.830 kb on - strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.886 kb on - strand, within SM_b20122 at 133.930 kb on - strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.035 kb on - strand, within SM_b20122 at 134.035 kb on - strand, within SM_b20122 at 134.035 kb on - strand, within SM_b20122 at 134.092 kb on + strand at 134.097 kb on + strand at 134.496 kb on + strand, within SM_b20123 at 134.497 kb on - strand, within SM_b20123 at 134.793 kb on - strand, within SM_b20123 at 134.793 kb on - strand, within SM_b20123 at 134.922 kb on - strand, within SM_b20123 at 135.070 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 132,848 + SM_b20119 0.67 +0.4 133,022 + SM_b22004 0.19 -0.1 133,025 + SM_b22004 0.20 +0.5 133,166 + SM_b22004 0.56 +2.1 133,167 - SM_b22004 0.56 +0.5 133,188 - SM_b22004 0.62 -4.7 133,368 - SM_b20121 0.31 -0.9 133,476 + SM_b20121 0.70 +0.2 133,609 - +1.0 133,610 + +0.1 133,654 + +1.0 133,654 + +0.8 133,654 + +0.0 133,689 + -1.0 133,689 + +2.9 133,702 + -0.4 133,723 - -1.3 133,723 - +1.4 133,830 - SM_b20122 0.27 -3.2 133,885 + SM_b20122 0.43 -0.6 133,885 + SM_b20122 0.43 -1.7 133,885 + SM_b20122 0.43 -0.7 133,886 - SM_b20122 0.43 +0.0 133,930 - SM_b20122 0.57 -2.0 134,034 + SM_b20122 0.88 -2.3 134,034 + SM_b20122 0.88 -0.0 134,034 + SM_b20122 0.88 -0.8 134,034 + SM_b20122 0.88 -0.2 134,034 + SM_b20122 0.88 +2.0 134,035 - SM_b20122 0.88 -0.2 134,035 - SM_b20122 0.88 -0.2 134,035 - SM_b20122 0.88 -1.0 134,092 + +3.5 134,097 + -2.3 134,496 + SM_b20123 0.26 +2.2 134,497 - SM_b20123 0.26 +1.0 134,793 - SM_b20123 0.59 -1.6 134,793 - SM_b20123 0.59 -1.5 134,922 - SM_b20123 0.74 -1.3 135,070 + -3.9
Or see this region's nucleotide sequence