Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20100 and SM_b20101 are separated by 60 nucleotides SM_b20101 and SM_b20102 are separated by 87 nucleotides
SM_b20100: SM_b20100 - dehydrogenase, at 108,761 to 109,747
_b20100
SM_b20101: SM_b20101 - hydroxyglutarate oxidase, at 109,808 to 111,088
_b20101
SM_b20102: SM_b20102 - acetoin catabolism regulatory protein, at 111,176 to 113,059
_b20102
Position (kb) 109
110
111
112 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 108.992 kb on + strand, within SM_b20100 at 109.233 kb on - strand, within SM_b20100 at 109.424 kb on + strand, within SM_b20100 at 109.425 kb on - strand, within SM_b20100 at 109.528 kb on - strand, within SM_b20100 at 109.571 kb on + strand, within SM_b20100 at 109.649 kb on + strand at 109.649 kb on + strand at 109.649 kb on + strand at 109.649 kb on + strand at 109.649 kb on + strand at 109.650 kb on - strand at 109.796 kb on + strand at 109.796 kb on + strand at 109.796 kb on + strand at 109.796 kb on + strand at 109.796 kb on + strand at 109.797 kb on - strand at 109.797 kb on - strand at 109.797 kb on - strand at 109.797 kb on - strand at 109.845 kb on - strand at 109.850 kb on + strand at 109.850 kb on + strand at 109.851 kb on - strand at 109.851 kb on - strand at 109.851 kb on - strand at 110.004 kb on - strand, within SM_b20101 at 110.004 kb on - strand, within SM_b20101 at 110.006 kb on + strand, within SM_b20101 at 110.006 kb on + strand, within SM_b20101 at 110.006 kb on + strand, within SM_b20101 at 110.186 kb on + strand, within SM_b20101 at 110.186 kb on + strand, within SM_b20101 at 110.186 kb on + strand, within SM_b20101 at 110.187 kb on - strand, within SM_b20101 at 110.228 kb on - strand, within SM_b20101 at 110.513 kb on + strand, within SM_b20101 at 110.517 kb on - strand, within SM_b20101 at 110.517 kb on - strand, within SM_b20101 at 110.562 kb on - strand, within SM_b20101 at 110.801 kb on + strand, within SM_b20101 at 110.802 kb on - strand, within SM_b20101 at 110.842 kb on - strand, within SM_b20101 at 110.842 kb on - strand, within SM_b20101 at 110.842 kb on - strand, within SM_b20101 at 111.087 kb on - strand at 111.111 kb on + strand at 111.111 kb on + strand at 111.317 kb on + strand at 111.317 kb on + strand at 111.318 kb on - strand at 111.318 kb on - strand at 111.319 kb on + strand at 111.340 kb on + strand at 111.340 kb on + strand at 111.341 kb on - strand at 111.425 kb on - strand, within SM_b20102 at 111.428 kb on + strand, within SM_b20102 at 111.428 kb on + strand, within SM_b20102 at 111.428 kb on + strand, within SM_b20102 at 111.428 kb on + strand, within SM_b20102 at 111.428 kb on + strand, within SM_b20102 at 111.429 kb on - strand, within SM_b20102 at 111.429 kb on - strand, within SM_b20102 at 111.519 kb on - strand, within SM_b20102 at 111.663 kb on - strand, within SM_b20102 at 111.809 kb on - strand, within SM_b20102 at 112.030 kb on + strand, within SM_b20102
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 108,992 + SM_b20100 0.23 +0.6 109,233 - SM_b20100 0.48 -1.6 109,424 + SM_b20100 0.67 +0.4 109,425 - SM_b20100 0.67 +2.1 109,528 - SM_b20100 0.78 +0.3 109,571 + SM_b20100 0.82 +2.2 109,649 + +1.1 109,649 + -0.1 109,649 + -2.1 109,649 + +2.8 109,649 + +1.7 109,650 - -2.7 109,796 + -0.0 109,796 + +0.0 109,796 + +1.0 109,796 + +0.0 109,796 + -0.3 109,797 - -0.6 109,797 - +1.0 109,797 - -0.2 109,797 - +0.6 109,845 - -1.3 109,850 + -1.5 109,850 + +0.6 109,851 - -2.6 109,851 - +1.2 109,851 - -1.1 110,004 - SM_b20101 0.15 +1.5 110,004 - SM_b20101 0.15 -2.9 110,006 + SM_b20101 0.15 +0.2 110,006 + SM_b20101 0.15 -0.3 110,006 + SM_b20101 0.15 +3.1 110,186 + SM_b20101 0.30 +1.3 110,186 + SM_b20101 0.30 -2.9 110,186 + SM_b20101 0.30 -1.6 110,187 - SM_b20101 0.30 -1.8 110,228 - SM_b20101 0.33 +0.3 110,513 + SM_b20101 0.55 +2.0 110,517 - SM_b20101 0.55 -0.3 110,517 - SM_b20101 0.55 -3.2 110,562 - SM_b20101 0.59 +0.1 110,801 + SM_b20101 0.78 -1.3 110,802 - SM_b20101 0.78 -0.4 110,842 - SM_b20101 0.81 +0.5 110,842 - SM_b20101 0.81 +1.3 110,842 - SM_b20101 0.81 -0.6 111,087 - -0.8 111,111 + -0.1 111,111 + -2.5 111,317 + +1.6 111,317 + +0.8 111,318 - +1.7 111,318 - -1.2 111,319 + -3.4 111,340 + -0.6 111,340 + -0.5 111,341 - -0.6 111,425 - SM_b20102 0.13 -1.0 111,428 + SM_b20102 0.13 +1.3 111,428 + SM_b20102 0.13 -3.3 111,428 + SM_b20102 0.13 -0.1 111,428 + SM_b20102 0.13 +0.0 111,428 + SM_b20102 0.13 +0.0 111,429 - SM_b20102 0.13 -0.1 111,429 - SM_b20102 0.13 -0.5 111,519 - SM_b20102 0.18 -0.2 111,663 - SM_b20102 0.26 +0.2 111,809 - SM_b20102 0.34 -0.6 112,030 + SM_b20102 0.45 -0.7
Or see this region's nucleotide sequence