Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20089 and SM_b20090 are separated by 3 nucleotides SM_b20090 and SM_b20091 are separated by 115 nucleotides SM_b20091 and SM_b20092 are separated by 85 nucleotides SM_b20092 and SM_b20093 are separated by 37 nucleotides SM_b20093 and SM_b20094 overlap by 106 nucleotides
SM_b20089: SM_b20089 - hypothetical protein, at 98,552 to 98,875
_b20089
SM_b20090: SM_b20090 - hypothetical protein, at 98,879 to 99,070
_b20090
SM_b20091: SM_b20091 - hypothetical protein, at 99,186 to 99,692
_b20091
SM_b20092: SM_b20092 - hypothetical protein, at 99,778 to 100,536
_b20092
SM_b20093: SM_b20093 - hypothetical protein, at 100,574 to 101,515
_b20093
SM_b20094: SM_b20094 - phospholipase, at 101,410 to 103,182
_b20094
Position (kb)
99
100
101 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 98.800 kb on - strand, within SM_b20089 at 98.800 kb on - strand, within SM_b20089 at 98.853 kb on + strand at 98.854 kb on - strand at 98.854 kb on - strand at 98.855 kb on + strand at 98.856 kb on - strand at 98.880 kb on + strand at 99.180 kb on + strand at 99.192 kb on - strand at 99.404 kb on + strand, within SM_b20091 at 99.405 kb on - strand, within SM_b20091 at 99.489 kb on + strand, within SM_b20091 at 99.491 kb on + strand, within SM_b20091 at 99.491 kb on + strand, within SM_b20091 at 99.491 kb on + strand, within SM_b20091 at 99.492 kb on - strand, within SM_b20091 at 99.492 kb on - strand, within SM_b20091 at 99.503 kb on + strand, within SM_b20091 at 99.504 kb on - strand, within SM_b20091 at 99.507 kb on + strand, within SM_b20091 at 99.779 kb on + strand at 99.780 kb on - strand at 99.780 kb on - strand at 99.780 kb on - strand at 99.880 kb on + strand, within SM_b20092 at 99.880 kb on + strand, within SM_b20092 at 99.881 kb on - strand, within SM_b20092 at 99.881 kb on - strand, within SM_b20092 at 100.001 kb on + strand, within SM_b20092 at 100.002 kb on - strand, within SM_b20092 at 100.274 kb on + strand, within SM_b20092 at 100.325 kb on - strand, within SM_b20092 at 100.342 kb on - strand, within SM_b20092 at 100.354 kb on + strand at 100.354 kb on + strand, within SM_b20092 at 100.487 kb on + strand at 100.641 kb on - strand at 100.661 kb on - strand at 100.742 kb on + strand, within SM_b20093 at 100.742 kb on + strand, within SM_b20093 at 100.743 kb on - strand, within SM_b20093 at 101.094 kb on - strand, within SM_b20093 at 101.282 kb on + strand, within SM_b20093 at 101.282 kb on + strand, within SM_b20093 at 101.283 kb on - strand, within SM_b20093 at 101.283 kb on - strand, within SM_b20093 at 101.283 kb on - strand, within SM_b20093 at 101.411 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 98,800 - SM_b20089 0.77 -0.4 98,800 - SM_b20089 0.77 +1.2 98,853 + +1.3 98,854 - -0.2 98,854 - -2.6 98,855 + +1.3 98,856 - +1.0 98,880 + -0.6 99,180 + +0.5 99,192 - -0.1 99,404 + SM_b20091 0.43 +0.6 99,405 - SM_b20091 0.43 -1.0 99,489 + SM_b20091 0.60 -1.4 99,491 + SM_b20091 0.60 -2.2 99,491 + SM_b20091 0.60 +1.0 99,491 + SM_b20091 0.60 -1.0 99,492 - SM_b20091 0.60 -0.4 99,492 - SM_b20091 0.60 -0.8 99,503 + SM_b20091 0.63 +0.5 99,504 - SM_b20091 0.63 +0.8 99,507 + SM_b20091 0.63 -0.6 99,779 + -1.6 99,780 - +1.0 99,780 - -1.6 99,780 - -0.1 99,880 + SM_b20092 0.13 -0.6 99,880 + SM_b20092 0.13 +1.4 99,881 - SM_b20092 0.14 +0.8 99,881 - SM_b20092 0.14 +0.1 100,001 + SM_b20092 0.29 -1.0 100,002 - SM_b20092 0.30 -2.5 100,274 + SM_b20092 0.65 +2.0 100,325 - SM_b20092 0.72 +2.0 100,342 - SM_b20092 0.74 +0.1 100,354 + +0.0 100,354 + SM_b20092 0.76 -2.6 100,487 + +3.3 100,641 - +2.0 100,661 - +0.1 100,742 + SM_b20093 0.18 -0.6 100,742 + SM_b20093 0.18 +2.3 100,743 - SM_b20093 0.18 -0.3 101,094 - SM_b20093 0.55 -0.5 101,282 + SM_b20093 0.75 -0.1 101,282 + SM_b20093 0.75 -1.9 101,283 - SM_b20093 0.75 +1.9 101,283 - SM_b20093 0.75 -0.1 101,283 - SM_b20093 0.75 +0.1 101,411 + +0.6
Or see this region's nucleotide sequence