Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20049 and SM_b20050 are separated by 143 nucleotides SM_b20050 and SM_b20054 are separated by 95 nucleotides
SM_b20049: SM_b20049 - elongation factor G, at 60,310 to 62,271
_b20049
SM_b20050: SM_b20050 - hypothetical protein, at 62,415 to 62,924
_b20050
SM_b20054: SM_b20054 - non-heme chloroperoxidase, at 63,020 to 63,847
_b20054
Position (kb) 62
63 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 61.780 kb on + strand, within SM_b20049 at 61.780 kb on + strand, within SM_b20049 at 61.781 kb on - strand, within SM_b20049 at 61.903 kb on + strand, within SM_b20049 at 62.213 kb on - strand at 62.283 kb on + strand at 62.284 kb on - strand at 62.376 kb on + strand at 62.605 kb on + strand, within SM_b20050 at 62.606 kb on - strand, within SM_b20050 at 62.719 kb on - strand, within SM_b20050 at 62.977 kb on - strand at 62.999 kb on - strand at 62.999 kb on - strand at 62.999 kb on - strand at 63.066 kb on + strand at 63.066 kb on + strand at 63.066 kb on + strand at 63.066 kb on + strand at 63.067 kb on - strand at 63.067 kb on - strand at 63.099 kb on + strand at 63.110 kb on + strand, within SM_b20054 at 63.351 kb on + strand, within SM_b20054 at 63.352 kb on - strand, within SM_b20054 at 63.397 kb on - strand, within SM_b20054 at 63.699 kb on + strand, within SM_b20054 at 63.737 kb on + strand, within SM_b20054 at 63.737 kb on + strand, within SM_b20054 at 63.807 kb on + strand at 63.807 kb on + strand at 63.807 kb on + strand at 63.895 kb on + strand at 63.895 kb on + strand at 63.895 kb on + strand at 63.896 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 61,780 + SM_b20049 0.75 +0.2 61,780 + SM_b20049 0.75 -2.2 61,781 - SM_b20049 0.75 +1.5 61,903 + SM_b20049 0.81 -0.2 62,213 - +0.1 62,283 + +2.6 62,284 - -2.5 62,376 + +0.4 62,605 + SM_b20050 0.37 -0.8 62,606 - SM_b20050 0.37 +0.0 62,719 - SM_b20050 0.60 -1.8 62,977 - +0.2 62,999 - +0.2 62,999 - +1.0 62,999 - +2.0 63,066 + +0.1 63,066 + +0.0 63,066 + +0.2 63,066 + +0.4 63,067 - +0.3 63,067 - +2.6 63,099 + +4.2 63,110 + SM_b20054 0.11 +0.4 63,351 + SM_b20054 0.40 -0.7 63,352 - SM_b20054 0.40 +1.5 63,397 - SM_b20054 0.46 -1.3 63,699 + SM_b20054 0.82 +1.0 63,737 + SM_b20054 0.87 +0.6 63,737 + SM_b20054 0.87 +0.0 63,807 + -0.1 63,807 + +1.0 63,807 + +0.6 63,895 + -0.6 63,895 + -3.4 63,895 + +0.5 63,896 - -1.0
Or see this region's nucleotide sequence