Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20042 and SM_b20043 are separated by 373 nucleotides SM_b20043 and SM_b20044 are separated by 460 nucleotides
SM_b20042: SM_b20042 - hypothetical protein, at 52,256 to 53,413
_b20042
SM_b20043: SM_b20043 - hypothetical protein, at 53,787 to 54,629
_b20043
SM_b20044: SM_b20044 - replication initiation protein RepC, at 55,090 to 56,400
_b20044
Position (kb) 53
54
55 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 53.213 kb on + strand, within SM_b20042 at 53.407 kb on - strand at 53.475 kb on + strand at 53.476 kb on - strand at 53.496 kb on + strand at 53.496 kb on + strand at 53.497 kb on - strand at 53.568 kb on + strand at 53.579 kb on + strand at 53.579 kb on + strand at 53.830 kb on + strand at 53.830 kb on + strand at 53.830 kb on + strand at 53.831 kb on - strand at 53.831 kb on - strand at 53.831 kb on - strand at 53.831 kb on - strand at 53.837 kb on + strand at 53.840 kb on - strand at 53.857 kb on - strand at 53.942 kb on + strand, within SM_b20043 at 53.942 kb on + strand, within SM_b20043 at 53.943 kb on - strand, within SM_b20043 at 54.014 kb on + strand, within SM_b20043 at 54.015 kb on - strand, within SM_b20043 at 54.015 kb on - strand, within SM_b20043 at 54.033 kb on - strand, within SM_b20043 at 54.177 kb on - strand, within SM_b20043 at 54.208 kb on + strand, within SM_b20043 at 54.232 kb on + strand, within SM_b20043 at 54.232 kb on + strand, within SM_b20043 at 54.286 kb on - strand, within SM_b20043 at 54.400 kb on - strand, within SM_b20043 at 54.400 kb on - strand, within SM_b20043 at 54.400 kb on - strand, within SM_b20043 at 54.440 kb on + strand, within SM_b20043 at 54.476 kb on - strand, within SM_b20043 at 54.525 kb on - strand, within SM_b20043 at 54.585 kb on + strand at 54.586 kb on - strand at 54.588 kb on - strand at 54.643 kb on - strand at 54.775 kb on + strand at 54.971 kb on - strand at 55.066 kb on + strand at 55.066 kb on + strand at 55.066 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 53,213 + SM_b20042 0.83 +1.0 53,407 - +0.4 53,475 + +2.2 53,476 - -0.2 53,496 + -0.1 53,496 + -1.9 53,497 - -1.3 53,568 + -0.8 53,579 + -1.8 53,579 + -2.2 53,830 + +1.0 53,830 + -2.2 53,830 + +1.1 53,831 - +1.1 53,831 - -3.5 53,831 - +1.2 53,831 - +1.8 53,837 + +1.5 53,840 - +0.6 53,857 - +1.1 53,942 + SM_b20043 0.18 -0.2 53,942 + SM_b20043 0.18 +3.0 53,943 - SM_b20043 0.19 +0.9 54,014 + SM_b20043 0.27 -1.7 54,015 - SM_b20043 0.27 +1.5 54,015 - SM_b20043 0.27 +2.3 54,033 - SM_b20043 0.29 +1.0 54,177 - SM_b20043 0.46 -2.9 54,208 + SM_b20043 0.50 -1.8 54,232 + SM_b20043 0.53 +2.3 54,232 + SM_b20043 0.53 -4.5 54,286 - SM_b20043 0.59 +0.7 54,400 - SM_b20043 0.73 -1.0 54,400 - SM_b20043 0.73 +0.9 54,400 - SM_b20043 0.73 -0.6 54,440 + SM_b20043 0.77 +1.7 54,476 - SM_b20043 0.82 +0.7 54,525 - SM_b20043 0.88 -0.3 54,585 + +1.3 54,586 - +1.5 54,588 - +1.4 54,643 - +0.2 54,775 + -1.6 54,971 - +0.2 55,066 + -4.3 55,066 + -0.9 55,066 + -0.4
Or see this region's nucleotide sequence