Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_821 and Pf1N1B4_822 are separated by 759 nucleotides Pf1N1B4_822 and Pf1N1B4_823 are separated by 201 nucleotides Pf1N1B4_823 and Pf1N1B4_824 are separated by 2 nucleotides
Pf1N1B4_821 - Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2), at 939,692 to 942,100
_821
Pf1N1B4_822 - ATP-dependent protease La (EC 3.4.21.53) Type I, at 942,860 to 945,277
_822
Pf1N1B4_823 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 945,479 to 945,871
_823
Pf1N1B4_824 - POSSIBLE MEMBRANE PROTEIN, at 945,874 to 946,518
_824
Position (kb) 942
943
944
945
946 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 941.909 kb on + strand at 941.921 kb on + strand at 941.993 kb on + strand at 941.993 kb on + strand at 941.994 kb on - strand at 941.994 kb on - strand at 941.994 kb on - strand at 941.994 kb on - strand at 942.017 kb on + strand at 942.017 kb on + strand at 942.017 kb on + strand at 942.017 kb on + strand at 942.017 kb on + strand at 942.018 kb on - strand at 942.018 kb on - strand at 942.018 kb on - strand at 942.018 kb on - strand at 942.018 kb on - strand at 942.018 kb on - strand at 942.018 kb on - strand at 942.071 kb on + strand at 942.071 kb on + strand at 942.071 kb on + strand at 942.071 kb on + strand at 942.071 kb on + strand at 942.072 kb on - strand at 942.072 kb on - strand at 942.072 kb on - strand at 942.072 kb on - strand at 942.072 kb on - strand at 942.083 kb on + strand at 942.084 kb on - strand at 942.084 kb on - strand at 942.084 kb on - strand at 942.191 kb on + strand at 942.224 kb on + strand at 942.234 kb on + strand at 942.234 kb on + strand at 942.234 kb on + strand at 942.362 kb on + strand at 942.363 kb on - strand at 942.363 kb on - strand at 942.704 kb on + strand at 942.704 kb on + strand at 942.708 kb on + strand at 942.708 kb on + strand at 942.708 kb on + strand at 943.991 kb on + strand, within Pf1N1B4_822 at 943.991 kb on + strand, within Pf1N1B4_822 at 944.097 kb on - strand, within Pf1N1B4_822 at 944.258 kb on + strand, within Pf1N1B4_822 at 945.439 kb on + strand at 945.439 kb on + strand at 945.439 kb on + strand at 945.439 kb on + strand at 945.440 kb on - strand at 945.440 kb on - strand at 945.440 kb on - strand at 945.477 kb on + strand at 945.477 kb on + strand at 945.477 kb on + strand at 945.478 kb on - strand at 945.500 kb on - strand at 945.704 kb on + strand, within Pf1N1B4_823 at 945.803 kb on + strand, within Pf1N1B4_823 at 945.803 kb on + strand, within Pf1N1B4_823 at 945.803 kb on + strand, within Pf1N1B4_823 at 945.804 kb on - strand, within Pf1N1B4_823 at 946.135 kb on + strand, within Pf1N1B4_824 at 946.135 kb on + strand, within Pf1N1B4_824
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 941,909 + +1.0 941,921 + +0.1 941,993 + +0.1 941,993 + -1.2 941,994 - +0.9 941,994 - -1.9 941,994 - -0.4 941,994 - +0.7 942,017 + +0.1 942,017 + -2.7 942,017 + +0.6 942,017 + +0.1 942,017 + +2.5 942,018 - +4.1 942,018 - -1.9 942,018 - -0.9 942,018 - -0.4 942,018 - -1.2 942,018 - -0.6 942,018 - +2.7 942,071 + -1.4 942,071 + +0.1 942,071 + +2.5 942,071 + +0.7 942,071 + +0.7 942,072 - +1.7 942,072 - +0.7 942,072 - +1.1 942,072 - -0.3 942,072 - +0.1 942,083 + +0.1 942,084 - -0.3 942,084 - +0.1 942,084 - +0.9 942,191 + -1.1 942,224 + -0.9 942,234 + -0.3 942,234 + +0.8 942,234 + +0.9 942,362 + +0.7 942,363 - +0.1 942,363 - +0.1 942,704 + -1.9 942,704 + +0.6 942,708 + +0.7 942,708 + -0.9 942,708 + -0.9 943,991 + Pf1N1B4_822 0.47 -2.4 943,991 + Pf1N1B4_822 0.47 -1.4 944,097 - Pf1N1B4_822 0.51 -0.4 944,258 + Pf1N1B4_822 0.58 +0.1 945,439 + +0.7 945,439 + -0.0 945,439 + +1.3 945,439 + -0.4 945,440 - -0.5 945,440 - -2.4 945,440 - -0.4 945,477 + -0.3 945,477 + -0.9 945,477 + +0.1 945,478 - +2.1 945,500 - +1.1 945,704 + Pf1N1B4_823 0.57 -0.4 945,803 + Pf1N1B4_823 0.82 -0.6 945,803 + Pf1N1B4_823 0.82 -1.2 945,803 + Pf1N1B4_823 0.82 -0.4 945,804 - Pf1N1B4_823 0.83 -2.2 946,135 + Pf1N1B4_824 0.40 -0.9 946,135 + Pf1N1B4_824 0.40 -0.5
Or see this region's nucleotide sequence