Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_622

Experiment: Fusidic 0.0625 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_621 and Pf1N1B4_622 are separated by 106 nucleotidesPf1N1B4_622 and Pf1N1B4_623 are separated by 33 nucleotides Pf1N1B4_621 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 717,626 to 720,478 _621 Pf1N1B4_622 - L-serine dehydratase (EC 4.3.1.17), at 720,585 to 721,961 _622 Pf1N1B4_623 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 721,995 to 723,119 _623 Position (kb) 720 721 722Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 719.648 kb on + strand, within Pf1N1B4_621at 719.648 kb on + strand, within Pf1N1B4_621at 719.648 kb on + strandat 719.648 kb on + strandat 719.648 kb on + strand, within Pf1N1B4_621at 719.649 kb on - strand, within Pf1N1B4_621at 719.649 kb on - strand, within Pf1N1B4_621at 719.649 kb on - strand, within Pf1N1B4_621at 719.649 kb on - strand, within Pf1N1B4_621at 719.649 kb on - strand, within Pf1N1B4_621at 719.903 kb on + strand, within Pf1N1B4_621at 719.903 kb on + strand, within Pf1N1B4_621at 719.903 kb on + strand, within Pf1N1B4_621at 719.903 kb on + strandat 719.903 kb on + strand, within Pf1N1B4_621at 719.903 kb on + strand, within Pf1N1B4_621at 719.903 kb on + strand, within Pf1N1B4_621at 719.903 kb on + strand, within Pf1N1B4_621at 719.903 kb on + strand, within Pf1N1B4_621at 719.903 kb on + strand, within Pf1N1B4_621at 719.904 kb on - strandat 719.904 kb on - strand, within Pf1N1B4_621at 719.904 kb on - strand, within Pf1N1B4_621at 719.904 kb on - strandat 719.904 kb on - strand, within Pf1N1B4_621at 719.904 kb on - strand, within Pf1N1B4_621at 719.911 kb on + strand, within Pf1N1B4_621at 719.911 kb on + strand, within Pf1N1B4_621at 719.911 kb on + strand, within Pf1N1B4_621at 719.911 kb on + strand, within Pf1N1B4_621at 719.911 kb on + strand, within Pf1N1B4_621at 719.912 kb on - strand, within Pf1N1B4_621at 719.912 kb on - strand, within Pf1N1B4_621at 719.969 kb on + strand, within Pf1N1B4_621at 719.969 kb on + strand, within Pf1N1B4_621at 719.969 kb on + strand, within Pf1N1B4_621at 719.969 kb on + strand, within Pf1N1B4_621at 719.969 kb on + strand, within Pf1N1B4_621at 719.970 kb on - strand, within Pf1N1B4_621at 720.008 kb on + strand, within Pf1N1B4_621at 720.008 kb on + strand, within Pf1N1B4_621at 720.008 kb on + strand, within Pf1N1B4_621at 720.008 kb on + strand, within Pf1N1B4_621at 720.009 kb on - strand, within Pf1N1B4_621at 720.009 kb on - strand, within Pf1N1B4_621at 720.009 kb on - strand, within Pf1N1B4_621at 720.009 kb on - strand, within Pf1N1B4_621at 720.009 kb on - strand, within Pf1N1B4_621at 720.341 kb on + strandat 720.342 kb on - strandat 720.369 kb on + strandat 720.392 kb on + strandat 720.392 kb on + strandat 720.392 kb on + strandat 720.392 kb on + strandat 720.470 kb on + strandat 720.470 kb on + strandat 720.470 kb on + strandat 720.471 kb on - strandat 720.471 kb on - strandat 720.471 kb on - strandat 720.471 kb on - strandat 720.547 kb on + strandat 720.547 kb on + strandat 720.547 kb on + strandat 720.547 kb on + strandat 720.547 kb on + strandat 720.548 kb on - strandat 720.548 kb on - strandat 720.548 kb on - strandat 720.560 kb on - strandat 720.560 kb on - strandat 720.560 kb on - strandat 720.560 kb on - strandat 720.560 kb on - strandat 720.565 kb on + strandat 720.565 kb on + strandat 720.566 kb on - strandat 720.566 kb on - strandat 720.566 kb on - strandat 720.820 kb on + strand, within Pf1N1B4_622at 720.821 kb on - strand, within Pf1N1B4_622at 720.948 kb on + strand, within Pf1N1B4_622at 721.015 kb on - strand, within Pf1N1B4_622at 721.017 kb on + strand, within Pf1N1B4_622at 721.018 kb on - strand, within Pf1N1B4_622at 721.018 kb on - strand, within Pf1N1B4_622at 721.143 kb on + strand, within Pf1N1B4_622at 721.144 kb on - strand, within Pf1N1B4_622at 721.144 kb on - strand, within Pf1N1B4_622at 721.144 kb on - strand, within Pf1N1B4_622at 721.144 kb on - strand, within Pf1N1B4_622at 721.396 kb on - strand, within Pf1N1B4_622at 721.396 kb on - strand, within Pf1N1B4_622at 721.491 kb on + strand, within Pf1N1B4_622at 721.491 kb on + strand, within Pf1N1B4_622at 721.491 kb on + strand, within Pf1N1B4_622at 721.491 kb on + strand, within Pf1N1B4_622at 721.491 kb on + strand, within Pf1N1B4_622at 721.492 kb on - strand, within Pf1N1B4_622at 721.492 kb on - strand, within Pf1N1B4_622at 721.492 kb on - strand, within Pf1N1B4_622at 721.492 kb on - strand, within Pf1N1B4_622at 721.492 kb on - strand, within Pf1N1B4_622at 721.492 kb on - strand, within Pf1N1B4_622at 721.492 kb on - strand, within Pf1N1B4_622at 721.492 kb on - strand, within Pf1N1B4_622at 721.492 kb on - strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strandat 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.494 kb on + strand, within Pf1N1B4_622at 721.495 kb on - strand, within Pf1N1B4_622at 721.495 kb on - strandat 721.495 kb on - strand, within Pf1N1B4_622at 721.495 kb on - strand, within Pf1N1B4_622at 721.495 kb on - strand, within Pf1N1B4_622at 721.495 kb on - strand, within Pf1N1B4_622at 721.495 kb on - strand, within Pf1N1B4_622at 721.495 kb on - strand, within Pf1N1B4_622at 721.534 kb on + strand, within Pf1N1B4_622at 721.535 kb on - strand, within Pf1N1B4_622at 721.572 kb on + strand, within Pf1N1B4_622at 721.572 kb on + strand, within Pf1N1B4_622at 721.572 kb on + strand, within Pf1N1B4_622at 721.573 kb on - strandat 721.573 kb on - strand, within Pf1N1B4_622at 721.573 kb on - strand, within Pf1N1B4_622at 721.573 kb on - strand, within Pf1N1B4_622at 721.590 kb on - strand, within Pf1N1B4_622at 721.590 kb on - strand, within Pf1N1B4_622at 721.765 kb on + strand, within Pf1N1B4_622at 721.765 kb on + strand, within Pf1N1B4_622at 721.765 kb on + strand, within Pf1N1B4_622at 721.765 kb on + strand, within Pf1N1B4_622at 721.765 kb on + strand, within Pf1N1B4_622at 721.766 kb on - strand, within Pf1N1B4_622at 721.766 kb on - strand, within Pf1N1B4_622at 721.766 kb on - strand, within Pf1N1B4_622at 721.766 kb on - strand, within Pf1N1B4_622at 721.766 kb on - strand, within Pf1N1B4_622at 721.911 kb on + strandat 721.912 kb on - strandat 721.912 kb on - strandat 721.912 kb on - strandat 721.912 kb on - strandat 721.995 kb on - strandat 722.095 kb on - strandat 722.095 kb on - strandat 722.510 kb on + strand, within Pf1N1B4_623at 722.510 kb on + strand, within Pf1N1B4_623at 722.511 kb on - strand, within Pf1N1B4_623at 722.892 kb on - strand, within Pf1N1B4_623

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml
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719,648 + Pf1N1B4_621 0.71 +1.9
719,648 + Pf1N1B4_621 0.71 +1.6
719,648 + +1.0
719,648 + -1.5
719,648 + Pf1N1B4_621 0.71 -0.6
719,649 - Pf1N1B4_621 0.71 -2.3
719,649 - Pf1N1B4_621 0.71 +0.4
719,649 - Pf1N1B4_621 0.71 +0.3
719,649 - Pf1N1B4_621 0.71 -0.5
719,649 - Pf1N1B4_621 0.71 +1.6
719,903 + Pf1N1B4_621 0.80 -0.2
719,903 + Pf1N1B4_621 0.80 +0.4
719,903 + Pf1N1B4_621 0.80 +0.4
719,903 + +0.0
719,903 + Pf1N1B4_621 0.80 -0.4
719,903 + Pf1N1B4_621 0.80 +2.3
719,903 + Pf1N1B4_621 0.80 +0.4
719,903 + Pf1N1B4_621 0.80 +0.0
719,903 + Pf1N1B4_621 0.80 +3.5
719,903 + Pf1N1B4_621 0.80 +2.2
719,904 - +0.6
719,904 - Pf1N1B4_621 0.80 +0.6
719,904 - Pf1N1B4_621 0.80 +0.0
719,904 - -1.0
719,904 - Pf1N1B4_621 0.80 +0.4
719,904 - Pf1N1B4_621 0.80 +0.0
719,911 + Pf1N1B4_621 0.80 +0.0
719,911 + Pf1N1B4_621 0.80 +1.2
719,911 + Pf1N1B4_621 0.80 +0.5
719,911 + Pf1N1B4_621 0.80 +0.7
719,911 + Pf1N1B4_621 0.80 +0.0
719,912 - Pf1N1B4_621 0.80 +2.2
719,912 - Pf1N1B4_621 0.80 -2.8
719,969 + Pf1N1B4_621 0.82 -2.0
719,969 + Pf1N1B4_621 0.82 -1.5
719,969 + Pf1N1B4_621 0.82 +0.5
719,969 + Pf1N1B4_621 0.82 +0.0
719,969 + Pf1N1B4_621 0.82 +0.6
719,970 - Pf1N1B4_621 0.82 -0.5
720,008 + Pf1N1B4_621 0.83 +1.0
720,008 + Pf1N1B4_621 0.83 -0.5
720,008 + Pf1N1B4_621 0.83 +2.5
720,008 + Pf1N1B4_621 0.83 -0.2
720,009 - Pf1N1B4_621 0.84 +1.9
720,009 - Pf1N1B4_621 0.84 +0.0
720,009 - Pf1N1B4_621 0.84 -1.5
720,009 - Pf1N1B4_621 0.84 -1.3
720,009 - Pf1N1B4_621 0.84 -0.7
720,341 + +1.0
720,342 - -2.0
720,369 + +3.1
720,392 + +1.6
720,392 + +3.0
720,392 + +0.6
720,392 + +0.6
720,470 + +1.0
720,470 + +0.5
720,470 + +1.6
720,471 - +2.7
720,471 - +2.6
720,471 - +0.2
720,471 - -3.3
720,547 + +0.9
720,547 + +0.0
720,547 + +0.0
720,547 + +0.0
720,547 + +0.5
720,548 - -1.0
720,548 - -1.0
720,548 - +0.0
720,560 - -0.4
720,560 - +0.5
720,560 - -0.2
720,560 - -1.5
720,560 - -0.6
720,565 + -0.4
720,565 + -0.5
720,566 - -0.8
720,566 - -1.5
720,566 - +0.0
720,820 + Pf1N1B4_622 0.17 -0.1
720,821 - Pf1N1B4_622 0.17 -0.1
720,948 + Pf1N1B4_622 0.26 -1.0
721,015 - Pf1N1B4_622 0.31 -2.0
721,017 + Pf1N1B4_622 0.31 -0.8
721,018 - Pf1N1B4_622 0.31 -1.8
721,018 - Pf1N1B4_622 0.31 +1.0
721,143 + Pf1N1B4_622 0.41 -1.3
721,144 - Pf1N1B4_622 0.41 +0.2
721,144 - Pf1N1B4_622 0.41 +1.5
721,144 - Pf1N1B4_622 0.41 -2.0
721,144 - Pf1N1B4_622 0.41 -1.5
721,396 - Pf1N1B4_622 0.59 +0.0
721,396 - Pf1N1B4_622 0.59 +0.3
721,491 + Pf1N1B4_622 0.66 -0.2
721,491 + Pf1N1B4_622 0.66 -1.0
721,491 + Pf1N1B4_622 0.66 +2.4
721,491 + Pf1N1B4_622 0.66 +0.3
721,491 + Pf1N1B4_622 0.66 -0.5
721,492 - Pf1N1B4_622 0.66 -1.7
721,492 - Pf1N1B4_622 0.66 -0.5
721,492 - Pf1N1B4_622 0.66 +0.0
721,492 - Pf1N1B4_622 0.66 +0.5
721,492 - Pf1N1B4_622 0.66 -2.0
721,492 - Pf1N1B4_622 0.66 -1.0
721,492 - Pf1N1B4_622 0.66 -1.0
721,492 - Pf1N1B4_622 0.66 +0.9
721,492 - Pf1N1B4_622 0.66 -0.4
721,494 + Pf1N1B4_622 0.66 -1.0
721,494 + Pf1N1B4_622 0.66 +0.8
721,494 + +1.0
721,494 + Pf1N1B4_622 0.66 +1.0
721,494 + Pf1N1B4_622 0.66 -2.3
721,494 + Pf1N1B4_622 0.66 +2.4
721,494 + Pf1N1B4_622 0.66 -2.0
721,494 + Pf1N1B4_622 0.66 +0.3
721,494 + Pf1N1B4_622 0.66 -0.5
721,494 + Pf1N1B4_622 0.66 +0.9
721,494 + Pf1N1B4_622 0.66 -0.3
721,494 + Pf1N1B4_622 0.66 +1.6
721,494 + Pf1N1B4_622 0.66 +0.5
721,495 - Pf1N1B4_622 0.66 -0.5
721,495 - +0.0
721,495 - Pf1N1B4_622 0.66 -1.5
721,495 - Pf1N1B4_622 0.66 -0.7
721,495 - Pf1N1B4_622 0.66 +0.8
721,495 - Pf1N1B4_622 0.66 -1.3
721,495 - Pf1N1B4_622 0.66 +0.0
721,495 - Pf1N1B4_622 0.66 -2.5
721,534 + Pf1N1B4_622 0.69 +2.4
721,535 - Pf1N1B4_622 0.69 +0.5
721,572 + Pf1N1B4_622 0.72 +1.0
721,572 + Pf1N1B4_622 0.72 +0.6
721,572 + Pf1N1B4_622 0.72 +1.6
721,573 - +0.0
721,573 - Pf1N1B4_622 0.72 +1.6
721,573 - Pf1N1B4_622 0.72 -1.8
721,573 - Pf1N1B4_622 0.72 +0.6
721,590 - Pf1N1B4_622 0.73 -0.4
721,590 - Pf1N1B4_622 0.73 +0.6
721,765 + Pf1N1B4_622 0.86 +0.6
721,765 + Pf1N1B4_622 0.86 +1.0
721,765 + Pf1N1B4_622 0.86 +1.4
721,765 + Pf1N1B4_622 0.86 +0.0
721,765 + Pf1N1B4_622 0.86 +1.6
721,766 - Pf1N1B4_622 0.86 +0.6
721,766 - Pf1N1B4_622 0.86 -0.8
721,766 - Pf1N1B4_622 0.86 -2.3
721,766 - Pf1N1B4_622 0.86 -1.1
721,766 - Pf1N1B4_622 0.86 -1.0
721,911 + -0.2
721,912 - +2.0
721,912 - +1.0
721,912 - +0.7
721,912 - +1.0
721,995 - -0.5
722,095 - -1.0
722,095 - -0.6
722,510 + Pf1N1B4_623 0.46 -2.0
722,510 + Pf1N1B4_623 0.46 -1.0
722,511 - Pf1N1B4_623 0.46 -1.3
722,892 - Pf1N1B4_623 0.80 -1.0

Or see this region's nucleotide sequence