Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_619 and Pf1N1B4_620 are separated by 457 nucleotides Pf1N1B4_620 and Pf1N1B4_621 are separated by 11 nucleotides Pf1N1B4_621 and Pf1N1B4_622 are separated by 106 nucleotides
Pf1N1B4_619 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 715,265 to 716,773
_619
Pf1N1B4_620 - Glycine cleavage system H protein, at 717,231 to 717,614
_620
Pf1N1B4_621 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 717,626 to 720,478
_621
Pf1N1B4_622 - L-serine dehydratase (EC 4.3.1.17), at 720,585 to 721,961
_622
Position (kb) 717
718
719
720
721 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 716.670 kb on - strand at 716.746 kb on + strand at 716.746 kb on + strand at 716.746 kb on + strand at 716.747 kb on - strand at 716.747 kb on - strand at 716.747 kb on - strand at 716.750 kb on + strand at 716.751 kb on - strand at 716.757 kb on + strand at 716.757 kb on + strand at 716.757 kb on + strand at 716.758 kb on - strand at 716.758 kb on - strand at 716.758 kb on - strand at 716.761 kb on + strand at 716.761 kb on + strand at 716.761 kb on + strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.843 kb on - strand at 716.846 kb on + strand at 716.846 kb on + strand at 716.846 kb on + strand at 716.847 kb on - strand at 716.858 kb on + strand at 716.859 kb on - strand at 716.871 kb on + strand at 716.871 kb on + strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 717.072 kb on - strand at 717.221 kb on - strand at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand at 717.580 kb on - strand at 717.637 kb on + strand at 717.637 kb on + strand at 717.638 kb on - strand at 717.869 kb on + strand at 717.869 kb on + strand at 717.869 kb on + strand at 717.870 kb on - strand at 717.870 kb on - strand at 717.870 kb on - strand at 717.885 kb on - strand at 717.898 kb on + strand at 717.899 kb on - strand at 717.899 kb on - strand at 717.899 kb on - strand at 717.899 kb on - strand at 717.954 kb on - strand, within Pf1N1B4_621 at 717.954 kb on - strand, within Pf1N1B4_621 at 717.963 kb on - strand, within Pf1N1B4_621 at 718.475 kb on + strand at 718.475 kb on + strand, within Pf1N1B4_621 at 718.592 kb on - strand, within Pf1N1B4_621 at 718.635 kb on + strand, within Pf1N1B4_621 at 718.635 kb on + strand at 718.635 kb on + strand, within Pf1N1B4_621 at 718.636 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.662 kb on - strand, within Pf1N1B4_621 at 718.799 kb on - strand, within Pf1N1B4_621 at 718.941 kb on + strand at 719.084 kb on + strand, within Pf1N1B4_621 at 719.369 kb on + strand, within Pf1N1B4_621 at 719.648 kb on + strand, within Pf1N1B4_621 at 719.648 kb on + strand, within Pf1N1B4_621 at 719.648 kb on + strand at 719.648 kb on + strand at 719.648 kb on + strand, within Pf1N1B4_621 at 719.649 kb on - strand, within Pf1N1B4_621 at 719.649 kb on - strand, within Pf1N1B4_621 at 719.649 kb on - strand, within Pf1N1B4_621 at 719.649 kb on - strand, within Pf1N1B4_621 at 719.649 kb on - strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.903 kb on + strand, within Pf1N1B4_621 at 719.904 kb on - strand at 719.904 kb on - strand, within Pf1N1B4_621 at 719.904 kb on - strand, within Pf1N1B4_621 at 719.904 kb on - strand at 719.904 kb on - strand, within Pf1N1B4_621 at 719.904 kb on - strand, within Pf1N1B4_621 at 719.911 kb on + strand, within Pf1N1B4_621 at 719.911 kb on + strand, within Pf1N1B4_621 at 719.911 kb on + strand, within Pf1N1B4_621 at 719.911 kb on + strand, within Pf1N1B4_621 at 719.911 kb on + strand, within Pf1N1B4_621 at 719.912 kb on - strand, within Pf1N1B4_621 at 719.912 kb on - strand, within Pf1N1B4_621 at 719.969 kb on + strand, within Pf1N1B4_621 at 719.969 kb on + strand, within Pf1N1B4_621 at 719.969 kb on + strand, within Pf1N1B4_621 at 719.969 kb on + strand, within Pf1N1B4_621 at 719.969 kb on + strand, within Pf1N1B4_621 at 719.970 kb on - strand, within Pf1N1B4_621 at 720.008 kb on + strand, within Pf1N1B4_621 at 720.008 kb on + strand, within Pf1N1B4_621 at 720.008 kb on + strand, within Pf1N1B4_621 at 720.008 kb on + strand, within Pf1N1B4_621 at 720.009 kb on - strand, within Pf1N1B4_621 at 720.009 kb on - strand, within Pf1N1B4_621 at 720.009 kb on - strand, within Pf1N1B4_621 at 720.009 kb on - strand, within Pf1N1B4_621 at 720.009 kb on - strand, within Pf1N1B4_621 at 720.341 kb on + strand at 720.342 kb on - strand at 720.369 kb on + strand at 720.392 kb on + strand at 720.392 kb on + strand at 720.392 kb on + strand at 720.392 kb on + strand at 720.470 kb on + strand at 720.470 kb on + strand at 720.470 kb on + strand at 720.471 kb on - strand at 720.471 kb on - strand at 720.471 kb on - strand at 720.471 kb on - strand at 720.547 kb on + strand at 720.547 kb on + strand at 720.547 kb on + strand at 720.547 kb on + strand at 720.547 kb on + strand at 720.548 kb on - strand at 720.548 kb on - strand at 720.548 kb on - strand at 720.560 kb on - strand at 720.560 kb on - strand at 720.560 kb on - strand at 720.560 kb on - strand at 720.560 kb on - strand at 720.565 kb on + strand at 720.565 kb on + strand at 720.566 kb on - strand at 720.566 kb on - strand at 720.566 kb on - strand at 720.820 kb on + strand, within Pf1N1B4_622 at 720.821 kb on - strand, within Pf1N1B4_622 at 720.948 kb on + strand, within Pf1N1B4_622 at 721.015 kb on - strand, within Pf1N1B4_622 at 721.017 kb on + strand, within Pf1N1B4_622 at 721.018 kb on - strand, within Pf1N1B4_622 at 721.018 kb on - strand, within Pf1N1B4_622 at 721.143 kb on + strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.396 kb on - strand, within Pf1N1B4_622 at 721.396 kb on - strand, within Pf1N1B4_622
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 716,670 - -1.0 716,746 + +0.8 716,746 + +0.0 716,746 + -0.5 716,747 - +0.5 716,747 - -0.5 716,747 - -0.4 716,750 + +0.5 716,751 - +0.9 716,757 + +0.4 716,757 + -0.5 716,757 + +0.0 716,758 - -0.5 716,758 - +2.1 716,758 - -1.0 716,761 + +3.5 716,761 + +0.4 716,761 + -1.1 716,762 - +0.4 716,762 - -0.3 716,762 - +0.6 716,762 - -2.0 716,762 - -2.3 716,762 - -0.7 716,843 - +0.0 716,846 + -1.5 716,846 + -2.0 716,846 + -0.5 716,847 - +0.6 716,858 + +0.0 716,859 - +2.3 716,871 + +0.0 716,871 + +0.1 716,872 - -0.4 716,872 - -1.3 716,872 - -1.5 716,872 - +0.3 716,872 - -1.0 717,072 - -1.5 717,221 - +1.4 717,426 + Pf1N1B4_620 0.51 +1.4 717,426 + Pf1N1B4_620 0.51 -0.3 717,426 + Pf1N1B4_620 0.51 +0.5 717,426 + Pf1N1B4_620 0.51 -0.3 717,427 - Pf1N1B4_620 0.51 -2.0 717,427 - Pf1N1B4_620 0.51 -2.3 717,427 - Pf1N1B4_620 0.51 +0.0 717,427 - +0.0 717,427 - Pf1N1B4_620 0.51 +0.9 717,427 - Pf1N1B4_620 0.51 +1.4 717,427 - Pf1N1B4_620 0.51 +1.5 717,427 - -0.4 717,580 - -0.5 717,637 + +1.6 717,637 + +0.0 717,638 - +1.0 717,869 + +0.6 717,869 + -1.0 717,869 + +1.6 717,870 - +0.0 717,870 - -1.0 717,870 - +0.5 717,885 - -1.1 717,898 + +1.0 717,899 - +0.5 717,899 - +2.0 717,899 - -2.0 717,899 - +1.8 717,954 - Pf1N1B4_621 0.11 +0.3 717,954 - Pf1N1B4_621 0.11 +0.6 717,963 - Pf1N1B4_621 0.12 -2.0 718,475 + +0.5 718,475 + Pf1N1B4_621 0.30 -1.5 718,592 - Pf1N1B4_621 0.34 +1.9 718,635 + Pf1N1B4_621 0.35 +0.4 718,635 + +1.0 718,635 + Pf1N1B4_621 0.35 +0.0 718,636 - Pf1N1B4_621 0.35 +0.6 718,662 - Pf1N1B4_621 0.36 -0.2 718,662 - Pf1N1B4_621 0.36 -1.0 718,662 - Pf1N1B4_621 0.36 -0.7 718,662 - Pf1N1B4_621 0.36 -0.6 718,662 - Pf1N1B4_621 0.36 +1.3 718,662 - Pf1N1B4_621 0.36 +0.0 718,799 - Pf1N1B4_621 0.41 +0.1 718,941 + +0.6 719,084 + Pf1N1B4_621 0.51 -0.7 719,369 + Pf1N1B4_621 0.61 +0.0 719,648 + Pf1N1B4_621 0.71 +1.9 719,648 + Pf1N1B4_621 0.71 +1.6 719,648 + +1.0 719,648 + -1.5 719,648 + Pf1N1B4_621 0.71 -0.6 719,649 - Pf1N1B4_621 0.71 -2.3 719,649 - Pf1N1B4_621 0.71 +0.4 719,649 - Pf1N1B4_621 0.71 +0.3 719,649 - Pf1N1B4_621 0.71 -0.5 719,649 - Pf1N1B4_621 0.71 +1.6 719,903 + Pf1N1B4_621 0.80 -0.2 719,903 + Pf1N1B4_621 0.80 +0.4 719,903 + Pf1N1B4_621 0.80 +0.4 719,903 + +0.0 719,903 + Pf1N1B4_621 0.80 -0.4 719,903 + Pf1N1B4_621 0.80 +2.3 719,903 + Pf1N1B4_621 0.80 +0.4 719,903 + Pf1N1B4_621 0.80 +0.0 719,903 + Pf1N1B4_621 0.80 +3.5 719,903 + Pf1N1B4_621 0.80 +2.2 719,904 - +0.6 719,904 - Pf1N1B4_621 0.80 +0.6 719,904 - Pf1N1B4_621 0.80 +0.0 719,904 - -1.0 719,904 - Pf1N1B4_621 0.80 +0.4 719,904 - Pf1N1B4_621 0.80 +0.0 719,911 + Pf1N1B4_621 0.80 +0.0 719,911 + Pf1N1B4_621 0.80 +1.2 719,911 + Pf1N1B4_621 0.80 +0.5 719,911 + Pf1N1B4_621 0.80 +0.7 719,911 + Pf1N1B4_621 0.80 +0.0 719,912 - Pf1N1B4_621 0.80 +2.2 719,912 - Pf1N1B4_621 0.80 -2.8 719,969 + Pf1N1B4_621 0.82 -2.0 719,969 + Pf1N1B4_621 0.82 -1.5 719,969 + Pf1N1B4_621 0.82 +0.5 719,969 + Pf1N1B4_621 0.82 +0.0 719,969 + Pf1N1B4_621 0.82 +0.6 719,970 - Pf1N1B4_621 0.82 -0.5 720,008 + Pf1N1B4_621 0.83 +1.0 720,008 + Pf1N1B4_621 0.83 -0.5 720,008 + Pf1N1B4_621 0.83 +2.5 720,008 + Pf1N1B4_621 0.83 -0.2 720,009 - Pf1N1B4_621 0.84 +1.9 720,009 - Pf1N1B4_621 0.84 +0.0 720,009 - Pf1N1B4_621 0.84 -1.5 720,009 - Pf1N1B4_621 0.84 -1.3 720,009 - Pf1N1B4_621 0.84 -0.7 720,341 + +1.0 720,342 - -2.0 720,369 + +3.1 720,392 + +1.6 720,392 + +3.0 720,392 + +0.6 720,392 + +0.6 720,470 + +1.0 720,470 + +0.5 720,470 + +1.6 720,471 - +2.7 720,471 - +2.6 720,471 - +0.2 720,471 - -3.3 720,547 + +0.9 720,547 + +0.0 720,547 + +0.0 720,547 + +0.0 720,547 + +0.5 720,548 - -1.0 720,548 - -1.0 720,548 - +0.0 720,560 - -0.4 720,560 - +0.5 720,560 - -0.2 720,560 - -1.5 720,560 - -0.6 720,565 + -0.4 720,565 + -0.5 720,566 - -0.8 720,566 - -1.5 720,566 - +0.0 720,820 + Pf1N1B4_622 0.17 -0.1 720,821 - Pf1N1B4_622 0.17 -0.1 720,948 + Pf1N1B4_622 0.26 -1.0 721,015 - Pf1N1B4_622 0.31 -2.0 721,017 + Pf1N1B4_622 0.31 -0.8 721,018 - Pf1N1B4_622 0.31 -1.8 721,018 - Pf1N1B4_622 0.31 +1.0 721,143 + Pf1N1B4_622 0.41 -1.3 721,144 - Pf1N1B4_622 0.41 +0.2 721,144 - Pf1N1B4_622 0.41 +1.5 721,144 - Pf1N1B4_622 0.41 -2.0 721,144 - Pf1N1B4_622 0.41 -1.5 721,396 - Pf1N1B4_622 0.59 +0.0 721,396 - Pf1N1B4_622 0.59 +0.3
Or see this region's nucleotide sequence