Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5848 and Pf1N1B4_5849 overlap by 1 nucleotides Pf1N1B4_5849 and Pf1N1B4_5850 overlap by 50 nucleotides Pf1N1B4_5850 and Pf1N1B4_5851 are separated by 247 nucleotides Pf1N1B4_5851 and Pf1N1B4_5852 are separated by 2 nucleotides Pf1N1B4_5852 and Pf1N1B4_5853 overlap by 4 nucleotides
Pf1N1B4_5848 - Precorrin-6A synthase (EC 2.1.1.152), at 282,097 to 282,852
_5848
Pf1N1B4_5849 - Probable phosphoglycerate mutase, at 282,852 to 283,337
_5849
Pf1N1B4_5850 - hypothetical protein, at 283,288 to 283,509
_5850
Pf1N1B4_5851 - Transcriptional regulator, RpiR family, at 283,757 to 284,611
_5851
Pf1N1B4_5852 - Amidases related to nicotinamidase, at 284,614 to 285,258
_5852
Pf1N1B4_5853 - Glutamine synthetase (EC 6.3.1.2), putative, at 285,255 to 286,586
_5853
Position (kb) 283
284
285 Strain fitness (log2 ratio) -2
-1
0
1
2 at 282.888 kb on + strand at 282.889 kb on - strand at 283.020 kb on - strand at 283.020 kb on - strand, within Pf1N1B4_5849 at 283.530 kb on + strand at 283.530 kb on + strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.683 kb on + strand at 283.683 kb on + strand at 283.801 kb on + strand at 283.824 kb on + strand at 283.825 kb on - strand at 284.036 kb on - strand, within Pf1N1B4_5851 at 284.058 kb on - strand, within Pf1N1B4_5851 at 284.058 kb on - strand, within Pf1N1B4_5851 at 284.118 kb on + strand, within Pf1N1B4_5851 at 284.119 kb on - strand, within Pf1N1B4_5851 at 284.119 kb on - strand, within Pf1N1B4_5851 at 284.402 kb on + strand, within Pf1N1B4_5851 at 284.403 kb on - strand at 284.609 kb on + strand at 284.610 kb on - strand at 285.154 kb on + strand, within Pf1N1B4_5852 at 285.155 kb on - strand at 285.172 kb on + strand, within Pf1N1B4_5852 at 285.444 kb on + strand, within Pf1N1B4_5853
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 282,888 + -0.6 282,889 - +0.3 283,020 - -1.4 283,020 - Pf1N1B4_5849 0.35 -0.0 283,530 + +0.1 283,530 + +0.6 283,531 - +2.2 283,531 - +0.6 283,683 + +2.1 283,683 + -0.4 283,801 + -1.4 283,824 + -0.7 283,825 - +0.3 284,036 - Pf1N1B4_5851 0.33 -0.6 284,058 - Pf1N1B4_5851 0.35 -0.8 284,058 - Pf1N1B4_5851 0.35 -0.4 284,118 + Pf1N1B4_5851 0.42 -0.7 284,119 - Pf1N1B4_5851 0.42 +0.6 284,119 - Pf1N1B4_5851 0.42 -2.7 284,402 + Pf1N1B4_5851 0.75 -0.4 284,403 - -0.4 284,609 + +0.8 284,610 - -1.6 285,154 + Pf1N1B4_5852 0.84 -2.7 285,155 - +1.2 285,172 + Pf1N1B4_5852 0.87 -1.8 285,444 + Pf1N1B4_5853 0.14 -0.4
Or see this region's nucleotide sequence