Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5847 and Pf1N1B4_5848 are separated by 147 nucleotides Pf1N1B4_5848 and Pf1N1B4_5849 overlap by 1 nucleotides Pf1N1B4_5849 and Pf1N1B4_5850 overlap by 50 nucleotides Pf1N1B4_5850 and Pf1N1B4_5851 are separated by 247 nucleotides
Pf1N1B4_5847 - transcriptional activator FtrA, at 280,975 to 281,949
_5847
Pf1N1B4_5848 - Precorrin-6A synthase (EC 2.1.1.152), at 282,097 to 282,852
_5848
Pf1N1B4_5849 - Probable phosphoglycerate mutase, at 282,852 to 283,337
_5849
Pf1N1B4_5850 - hypothetical protein, at 283,288 to 283,509
_5850
Pf1N1B4_5851 - Transcriptional regulator, RpiR family, at 283,757 to 284,611
_5851
Position (kb) 282
283 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 281.197 kb on - strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand at 281.425 kb on + strand, within Pf1N1B4_5847 at 281.425 kb on + strand at 281.425 kb on + strand, within Pf1N1B4_5847 at 281.933 kb on + strand at 281.934 kb on - strand at 281.934 kb on - strand at 282.116 kb on + strand at 282.116 kb on + strand at 282.116 kb on + strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.120 kb on + strand at 282.121 kb on - strand at 282.121 kb on - strand at 282.242 kb on + strand, within Pf1N1B4_5848 at 282.243 kb on - strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand at 282.393 kb on + strand at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.495 kb on + strand, within Pf1N1B4_5848 at 282.496 kb on - strand at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.504 kb on - strand, within Pf1N1B4_5848 at 282.504 kb on - strand, within Pf1N1B4_5848 at 282.623 kb on + strand at 282.692 kb on + strand, within Pf1N1B4_5848 at 282.692 kb on + strand, within Pf1N1B4_5848 at 282.693 kb on - strand, within Pf1N1B4_5848 at 282.888 kb on + strand at 282.889 kb on - strand at 283.020 kb on - strand at 283.020 kb on - strand, within Pf1N1B4_5849 at 283.530 kb on + strand at 283.530 kb on + strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.683 kb on + strand at 283.683 kb on + strand at 283.801 kb on + strand at 283.824 kb on + strand at 283.825 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 281,197 - Pf1N1B4_5847 0.23 -0.4 281,402 + Pf1N1B4_5847 0.44 +0.9 281,402 + Pf1N1B4_5847 0.44 +1.2 281,402 + Pf1N1B4_5847 0.44 -2.0 281,402 + Pf1N1B4_5847 0.44 +0.3 281,402 + Pf1N1B4_5847 0.44 -0.0 281,403 - Pf1N1B4_5847 0.44 +0.3 281,403 - Pf1N1B4_5847 0.44 -0.0 281,403 - Pf1N1B4_5847 0.44 +0.3 281,403 - -0.4 281,425 + Pf1N1B4_5847 0.46 -2.0 281,425 + +1.6 281,425 + Pf1N1B4_5847 0.46 +1.5 281,933 + -2.0 281,934 - +1.2 281,934 - -1.4 282,116 + -1.4 282,116 + +0.6 282,116 + +1.2 282,117 - +0.6 282,117 - +0.6 282,117 - -0.2 282,117 - -0.1 282,117 - +1.4 282,117 - +0.6 282,120 + +1.7 282,121 - -0.4 282,121 - +0.7 282,242 + Pf1N1B4_5848 0.19 +1.5 282,243 - Pf1N1B4_5848 0.19 -0.7 282,393 + Pf1N1B4_5848 0.39 +0.3 282,393 + Pf1N1B4_5848 0.39 -0.8 282,393 + Pf1N1B4_5848 0.39 -0.4 282,393 + -0.4 282,393 + -2.0 282,393 + Pf1N1B4_5848 0.39 -1.4 282,393 + Pf1N1B4_5848 0.39 -0.0 282,394 - Pf1N1B4_5848 0.39 -2.0 282,394 - Pf1N1B4_5848 0.39 -0.4 282,394 - +1.2 282,394 - Pf1N1B4_5848 0.39 -3.4 282,394 - Pf1N1B4_5848 0.39 -0.0 282,394 - +0.2 282,394 - Pf1N1B4_5848 0.39 -2.4 282,495 + Pf1N1B4_5848 0.53 -2.0 282,496 - -1.0 282,503 + Pf1N1B4_5848 0.54 -2.2 282,503 + Pf1N1B4_5848 0.54 -0.1 282,503 + Pf1N1B4_5848 0.54 +0.9 282,503 + Pf1N1B4_5848 0.54 -2.0 282,503 + Pf1N1B4_5848 0.54 -0.4 282,504 - Pf1N1B4_5848 0.54 +0.2 282,504 - Pf1N1B4_5848 0.54 -1.0 282,623 + -1.4 282,692 + Pf1N1B4_5848 0.79 +0.3 282,692 + Pf1N1B4_5848 0.79 -2.4 282,693 - Pf1N1B4_5848 0.79 -0.6 282,888 + -0.6 282,889 - +0.3 283,020 - -1.4 283,020 - Pf1N1B4_5849 0.35 -0.0 283,530 + +0.1 283,530 + +0.6 283,531 - +2.2 283,531 - +0.6 283,683 + +2.1 283,683 + -0.4 283,801 + -1.4 283,824 + -0.7 283,825 - +0.3
Or see this region's nucleotide sequence