Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5822 and Pf1N1B4_5823 overlap by 1 nucleotides Pf1N1B4_5823 and Pf1N1B4_5824 are separated by 5 nucleotides Pf1N1B4_5824 and Pf1N1B4_5825 overlap by 4 nucleotides Pf1N1B4_5825 and Pf1N1B4_5826 overlap by 4 nucleotides Pf1N1B4_5826 and Pf1N1B4_5827 are separated by 8 nucleotides
Pf1N1B4_5822 - Na(+) H(+) antiporter subunit A / Na(+) H(+) antiporter subunit B, at 256,081 to 258,957
_5822
Pf1N1B4_5823 - Na(+) H(+) antiporter subunit C, at 258,957 to 259,301
_5823
Pf1N1B4_5824 - Na(+) H(+) antiporter subunit D, at 259,307 to 260,983
_5824
Pf1N1B4_5825 - Na(+) H(+) antiporter subunit E, at 260,980 to 261,468
_5825
Pf1N1B4_5826 - Na(+) H(+) antiporter subunit F, at 261,465 to 261,743
_5826
Pf1N1B4_5827 - Na(+) H(+) antiporter subunit G, at 261,752 to 262,102
_5827
Position (kb) 259
260
261 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.620 kb on - strand, within Pf1N1B4_5822 at 258.829 kb on + strand at 258.898 kb on - strand at 259.065 kb on + strand, within Pf1N1B4_5823 at 259.292 kb on + strand at 259.496 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.800 kb on - strand, within Pf1N1B4_5824 at 259.856 kb on + strand, within Pf1N1B4_5824 at 260.078 kb on + strand, within Pf1N1B4_5824 at 260.315 kb on + strand, within Pf1N1B4_5824 at 260.316 kb on - strand, within Pf1N1B4_5824 at 260.906 kb on + strand at 260.906 kb on + strand at 260.906 kb on + strand at 260.906 kb on + strand at 260.907 kb on - strand at 260.907 kb on - strand at 260.907 kb on - strand at 261.068 kb on + strand, within Pf1N1B4_5825 at 261.595 kb on - strand, within Pf1N1B4_5826 at 261.595 kb on - strand, within Pf1N1B4_5826 at 261.595 kb on - strand, within Pf1N1B4_5826 at 261.808 kb on + strand, within Pf1N1B4_5827 at 261.923 kb on + strand, within Pf1N1B4_5827
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 258,619 + Pf1N1B4_5822 0.88 -2.0 258,619 + Pf1N1B4_5822 0.88 +0.6 258,619 + Pf1N1B4_5822 0.88 +0.6 258,620 - Pf1N1B4_5822 0.88 +2.1 258,829 + -0.1 258,898 - -1.7 259,065 + Pf1N1B4_5823 0.31 -0.1 259,292 + +0.7 259,496 + Pf1N1B4_5824 0.11 -0.4 259,799 + Pf1N1B4_5824 0.29 +0.2 259,799 + Pf1N1B4_5824 0.29 -1.8 259,799 + Pf1N1B4_5824 0.29 -1.0 259,799 + -0.4 259,799 + Pf1N1B4_5824 0.29 +0.6 259,799 + Pf1N1B4_5824 0.29 -3.4 259,799 + Pf1N1B4_5824 0.29 -2.4 259,800 - Pf1N1B4_5824 0.29 -0.4 259,856 + Pf1N1B4_5824 0.33 -2.0 260,078 + Pf1N1B4_5824 0.46 -0.4 260,315 + Pf1N1B4_5824 0.60 -1.0 260,316 - Pf1N1B4_5824 0.60 -2.2 260,906 + -0.4 260,906 + -4.0 260,906 + -0.4 260,906 + -0.4 260,907 - -0.8 260,907 - +0.6 260,907 - -2.0 261,068 + Pf1N1B4_5825 0.18 +1.0 261,595 - Pf1N1B4_5826 0.47 -2.8 261,595 - Pf1N1B4_5826 0.47 +0.1 261,595 - Pf1N1B4_5826 0.47 +0.2 261,808 + Pf1N1B4_5827 0.16 -2.0 261,923 + Pf1N1B4_5827 0.49 -1.0
Or see this region's nucleotide sequence