Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_562 and Pf1N1B4_563 overlap by 4 nucleotides Pf1N1B4_563 and Pf1N1B4_564 are separated by 233 nucleotides Pf1N1B4_564 and Pf1N1B4_565 are separated by 165 nucleotides
Pf1N1B4_562 - Histidine transporter, periplasmic histidine-binding protein, at 658,797 to 659,810
_562
Pf1N1B4_563 - RNA polymerase sigma-70 factor, at 659,807 to 660,355
_563
Pf1N1B4_564 - ABC-type phosphate/phosphonate transport system, periplasmic component, at 660,589 to 661,353
_564
Pf1N1B4_565 - Fatty acid desaturase, at 661,519 to 662,460
_565
Position (kb) 660
661
662 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.893 kb on - strand, within Pf1N1B4_563 at 659.893 kb on - strand, within Pf1N1B4_563 at 659.943 kb on + strand, within Pf1N1B4_563 at 659.943 kb on + strand, within Pf1N1B4_563 at 660.138 kb on + strand, within Pf1N1B4_563 at 660.355 kb on + strand at 660.355 kb on + strand at 660.355 kb on + strand at 660.355 kb on + strand at 660.355 kb on + strand at 660.356 kb on - strand at 660.356 kb on - strand at 660.412 kb on - strand at 660.412 kb on - strand at 660.412 kb on - strand at 660.412 kb on - strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.486 kb on - strand at 660.486 kb on - strand at 660.651 kb on - strand at 660.785 kb on + strand, within Pf1N1B4_564 at 660.785 kb on + strand, within Pf1N1B4_564 at 660.786 kb on - strand, within Pf1N1B4_564 at 660.863 kb on + strand, within Pf1N1B4_564 at 661.102 kb on + strand, within Pf1N1B4_564 at 661.102 kb on + strand, within Pf1N1B4_564 at 661.139 kb on - strand, within Pf1N1B4_564 at 661.349 kb on + strand at 661.349 kb on + strand at 661.349 kb on + strand at 661.350 kb on - strand at 661.350 kb on - strand at 661.350 kb on - strand at 661.350 kb on - strand at 661.350 kb on - strand at 661.357 kb on + strand at 661.357 kb on + strand at 661.357 kb on + strand at 661.357 kb on + strand at 661.358 kb on - strand at 661.358 kb on - strand at 661.358 kb on - strand at 661.392 kb on - strand at 661.403 kb on - strand at 661.403 kb on - strand at 661.403 kb on - strand at 661.403 kb on - strand at 661.403 kb on - strand at 661.469 kb on + strand at 661.469 kb on + strand at 661.469 kb on + strand at 661.470 kb on - strand at 661.524 kb on + strand at 661.524 kb on + strand at 661.524 kb on + strand at 661.524 kb on + strand at 661.525 kb on - strand at 661.525 kb on - strand at 661.583 kb on + strand at 661.584 kb on - strand at 661.584 kb on - strand at 661.584 kb on - strand at 661.584 kb on - strand at 661.641 kb on - strand, within Pf1N1B4_565 at 661.659 kb on - strand, within Pf1N1B4_565 at 661.659 kb on - strand, within Pf1N1B4_565 at 661.787 kb on + strand, within Pf1N1B4_565 at 661.787 kb on + strand, within Pf1N1B4_565 at 661.788 kb on - strand, within Pf1N1B4_565 at 661.788 kb on - strand at 661.788 kb on - strand, within Pf1N1B4_565 at 661.788 kb on - strand, within Pf1N1B4_565 at 661.788 kb on - strand, within Pf1N1B4_565 at 661.788 kb on - strand, within Pf1N1B4_565 at 661.790 kb on + strand, within Pf1N1B4_565 at 661.790 kb on + strand, within Pf1N1B4_565 at 661.790 kb on + strand, within Pf1N1B4_565 at 661.790 kb on + strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.791 kb on - strand, within Pf1N1B4_565 at 661.841 kb on + strand, within Pf1N1B4_565 at 661.911 kb on + strand, within Pf1N1B4_565 at 662.088 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 659,892 + Pf1N1B4_563 0.15 +2.4 659,892 + Pf1N1B4_563 0.15 +1.3 659,892 + Pf1N1B4_563 0.15 -1.6 659,892 + Pf1N1B4_563 0.15 +0.7 659,893 - Pf1N1B4_563 0.16 -0.3 659,893 - Pf1N1B4_563 0.16 +1.0 659,943 + Pf1N1B4_563 0.25 +0.0 659,943 + Pf1N1B4_563 0.25 -2.1 660,138 + Pf1N1B4_563 0.60 +1.8 660,355 + -0.6 660,355 + +0.0 660,355 + -1.3 660,355 + -0.5 660,355 + +0.8 660,356 - +0.0 660,356 - -1.6 660,412 - -3.0 660,412 - +0.9 660,412 - -0.3 660,412 - +1.1 660,485 + +1.0 660,485 + +1.6 660,485 + +1.0 660,485 + +0.6 660,485 + +1.3 660,485 + +0.0 660,485 + +0.4 660,485 + +2.0 660,485 + -1.6 660,486 - +0.0 660,486 - +0.0 660,651 - +1.4 660,785 + Pf1N1B4_564 0.26 +0.7 660,785 + Pf1N1B4_564 0.26 -1.5 660,786 - Pf1N1B4_564 0.26 -1.0 660,863 + Pf1N1B4_564 0.36 -1.0 661,102 + Pf1N1B4_564 0.67 -1.3 661,102 + Pf1N1B4_564 0.67 -1.3 661,139 - Pf1N1B4_564 0.72 -1.3 661,349 + -1.0 661,349 + +1.8 661,349 + +0.8 661,350 - +2.2 661,350 - +0.3 661,350 - +0.0 661,350 - +3.4 661,350 - -1.0 661,357 + +1.0 661,357 + +0.4 661,357 + +0.0 661,357 + +0.4 661,358 - +0.0 661,358 - -0.6 661,358 - +1.0 661,392 - -1.6 661,403 - +0.8 661,403 - -0.6 661,403 - +1.4 661,403 - -0.7 661,403 - +1.6 661,469 + -1.6 661,469 + -1.6 661,469 + +0.8 661,470 - +1.4 661,524 + -2.0 661,524 + -1.6 661,524 + -1.4 661,524 + +0.3 661,525 - -1.6 661,525 - +0.6 661,583 + -0.8 661,584 - +0.5 661,584 - +0.9 661,584 - +0.0 661,584 - +0.4 661,641 - Pf1N1B4_565 0.13 +0.0 661,659 - Pf1N1B4_565 0.15 +1.6 661,659 - Pf1N1B4_565 0.15 +1.3 661,787 + Pf1N1B4_565 0.28 +1.0 661,787 + Pf1N1B4_565 0.28 -0.5 661,788 - Pf1N1B4_565 0.29 +0.6 661,788 - -1.0 661,788 - Pf1N1B4_565 0.29 +2.0 661,788 - Pf1N1B4_565 0.29 -0.6 661,788 - Pf1N1B4_565 0.29 +0.6 661,788 - Pf1N1B4_565 0.29 +0.4 661,790 + Pf1N1B4_565 0.29 -1.6 661,790 + Pf1N1B4_565 0.29 +0.0 661,790 + Pf1N1B4_565 0.29 -1.6 661,790 + Pf1N1B4_565 0.29 +0.0 661,791 - Pf1N1B4_565 0.29 +0.6 661,791 - Pf1N1B4_565 0.29 -2.0 661,791 - Pf1N1B4_565 0.29 +0.0 661,791 - Pf1N1B4_565 0.29 -1.2 661,791 - Pf1N1B4_565 0.29 -0.5 661,791 - Pf1N1B4_565 0.29 -0.6 661,791 - Pf1N1B4_565 0.29 +0.0 661,841 + Pf1N1B4_565 0.34 +1.4 661,911 + Pf1N1B4_565 0.42 -0.6 662,088 - +1.0
Or see this region's nucleotide sequence