Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5623 and Pf1N1B4_5624 are separated by 339 nucleotides Pf1N1B4_5624 and Pf1N1B4_5625 are separated by 96 nucleotides Pf1N1B4_5625 and Pf1N1B4_5626 are separated by 135 nucleotides
Pf1N1B4_5623 - hypothetical protein, at 49,605 to 50,657
_5623
Pf1N1B4_5624 - Lipase precursor (EC 3.1.1.3), at 50,997 to 51,878
_5624
Pf1N1B4_5625 - Peptidyl-prolyl cis-trans isomerase PpiC (EC 5.2.1.8), at 51,975 to 52,256
_5625
Pf1N1B4_5626 - ABC transporter, periplasmic substrate-binding protein, at 52,392 to 54,260
_5626
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.043 kb on - strand, within Pf1N1B4_5623 at 50.298 kb on + strand, within Pf1N1B4_5623 at 50.339 kb on - strand at 50.348 kb on - strand, within Pf1N1B4_5623 at 50.348 kb on - strand at 50.389 kb on - strand, within Pf1N1B4_5623 at 50.389 kb on - strand, within Pf1N1B4_5623 at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.615 kb on - strand at 50.647 kb on + strand at 50.651 kb on + strand at 50.651 kb on + strand at 50.680 kb on + strand at 50.792 kb on - strand at 50.823 kb on + strand at 50.880 kb on - strand at 50.947 kb on - strand at 51.153 kb on - strand, within Pf1N1B4_5624 at 51.585 kb on + strand, within Pf1N1B4_5624 at 51.586 kb on - strand, within Pf1N1B4_5624 at 51.682 kb on - strand, within Pf1N1B4_5624 at 51.682 kb on - strand, within Pf1N1B4_5624 at 51.837 kb on + strand at 51.838 kb on - strand at 51.838 kb on - strand at 51.916 kb on + strand at 51.966 kb on + strand at 52.323 kb on - strand at 52.438 kb on + strand at 52.441 kb on + strand at 52.441 kb on + strand at 52.441 kb on + strand at 52.442 kb on - strand at 52.488 kb on + strand at 52.604 kb on + strand, within Pf1N1B4_5626 at 52.605 kb on - strand, within Pf1N1B4_5626 at 52.660 kb on - strand, within Pf1N1B4_5626 at 52.660 kb on - strand, within Pf1N1B4_5626 at 52.660 kb on - strand, within Pf1N1B4_5626
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 50,006 - Pf1N1B4_5623 0.38 +1.0 50,006 - Pf1N1B4_5623 0.38 +1.6 50,006 - Pf1N1B4_5623 0.38 +1.1 50,043 - Pf1N1B4_5623 0.42 -1.4 50,298 + Pf1N1B4_5623 0.66 +1.1 50,339 - +0.6 50,348 - Pf1N1B4_5623 0.71 +0.4 50,348 - -2.0 50,389 - Pf1N1B4_5623 0.74 -0.8 50,389 - Pf1N1B4_5623 0.74 +0.6 50,615 - +1.6 50,615 - -1.4 50,615 - -1.4 50,615 - -2.8 50,615 - +1.9 50,615 - +0.3 50,615 - -1.2 50,615 - +1.9 50,647 + +1.6 50,651 + +1.1 50,651 + +0.6 50,680 + +0.8 50,792 - +1.1 50,823 + -0.2 50,880 - +0.4 50,947 - +0.9 51,153 - Pf1N1B4_5624 0.18 -2.4 51,585 + Pf1N1B4_5624 0.67 +0.6 51,586 - Pf1N1B4_5624 0.67 -1.7 51,682 - Pf1N1B4_5624 0.78 +0.4 51,682 - Pf1N1B4_5624 0.78 -0.4 51,837 + +0.3 51,838 - +1.1 51,838 - -2.4 51,916 + +1.1 51,966 + +2.1 52,323 - +0.4 52,438 + -2.8 52,441 + -0.4 52,441 + +1.1 52,441 + +1.4 52,442 - +0.7 52,488 + -0.1 52,604 + Pf1N1B4_5626 0.11 +0.0 52,605 - Pf1N1B4_5626 0.11 -1.0 52,660 - Pf1N1B4_5626 0.14 -0.2 52,660 - Pf1N1B4_5626 0.14 -0.4 52,660 - Pf1N1B4_5626 0.14 +1.7
Or see this region's nucleotide sequence