Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5357 and Pf1N1B4_5358 are separated by 540 nucleotides Pf1N1B4_5358 and Pf1N1B4_5359 are separated by 80 nucleotides Pf1N1B4_5359 and Pf1N1B4_5360 are separated by 337 nucleotides
Pf1N1B4_5357 - hypothetical protein, at 94,130 to 94,306
_5357
Pf1N1B4_5358 - hypothetical protein, at 94,847 to 95,005
_5358
Pf1N1B4_5359 - hypothetical protein, at 95,086 to 95,373
_5359
Pf1N1B4_5360 - hypothetical protein, at 95,711 to 96,091
_5360
Position (kb)
95
96 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 94.086 kb on + strand at 94.086 kb on + strand at 94.096 kb on + strand at 94.172 kb on + strand, within Pf1N1B4_5357 at 94.172 kb on + strand, within Pf1N1B4_5357 at 94.173 kb on - strand, within Pf1N1B4_5357 at 94.333 kb on + strand at 94.334 kb on - strand at 94.344 kb on + strand at 94.345 kb on - strand at 94.361 kb on + strand at 94.386 kb on + strand at 94.387 kb on - strand at 94.528 kb on - strand at 94.530 kb on + strand at 94.530 kb on + strand at 94.530 kb on + strand at 94.530 kb on + strand at 94.530 kb on + strand at 94.530 kb on + strand at 94.531 kb on - strand at 94.531 kb on - strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.533 kb on - strand at 94.600 kb on - strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.663 kb on - strand at 94.663 kb on - strand at 94.707 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.753 kb on - strand at 94.753 kb on - strand at 94.825 kb on + strand at 94.825 kb on + strand at 94.825 kb on + strand at 94.825 kb on + strand at 94.825 kb on + strand at 94.862 kb on + strand at 94.863 kb on - strand, within Pf1N1B4_5358 at 94.939 kb on - strand, within Pf1N1B4_5358 at 94.996 kb on + strand at 95.073 kb on + strand at 95.073 kb on + strand at 95.079 kb on + strand at 95.079 kb on + strand at 95.080 kb on - strand at 95.080 kb on - strand at 95.145 kb on + strand, within Pf1N1B4_5359 at 95.145 kb on + strand, within Pf1N1B4_5359 at 95.160 kb on + strand, within Pf1N1B4_5359 at 95.213 kb on + strand, within Pf1N1B4_5359 at 95.213 kb on + strand, within Pf1N1B4_5359 at 95.213 kb on + strand, within Pf1N1B4_5359 at 95.213 kb on + strand, within Pf1N1B4_5359 at 95.214 kb on - strand, within Pf1N1B4_5359 at 95.371 kb on + strand at 95.372 kb on - strand at 95.430 kb on + strand at 95.620 kb on + strand at 95.620 kb on + strand at 95.734 kb on - strand at 95.807 kb on + strand, within Pf1N1B4_5360 at 95.807 kb on + strand, within Pf1N1B4_5360 at 95.808 kb on - strand, within Pf1N1B4_5360 at 95.808 kb on - strand, within Pf1N1B4_5360 at 95.808 kb on - strand at 95.808 kb on - strand at 95.808 kb on - strand at 95.808 kb on - strand, within Pf1N1B4_5360 at 95.808 kb on - strand, within Pf1N1B4_5360 at 95.808 kb on - strand, within Pf1N1B4_5360 at 95.808 kb on - strand, within Pf1N1B4_5360 at 95.808 kb on - strand, within Pf1N1B4_5360 at 95.808 kb on - strand, within Pf1N1B4_5360 at 95.834 kb on + strand, within Pf1N1B4_5360 at 95.835 kb on - strand, within Pf1N1B4_5360 at 95.835 kb on - strand, within Pf1N1B4_5360 at 95.912 kb on + strand, within Pf1N1B4_5360 at 95.912 kb on + strand, within Pf1N1B4_5360 at 95.912 kb on + strand, within Pf1N1B4_5360 at 95.913 kb on - strand, within Pf1N1B4_5360 at 95.913 kb on - strand, within Pf1N1B4_5360 at 95.913 kb on - strand, within Pf1N1B4_5360 at 96.042 kb on - strand, within Pf1N1B4_5360 at 96.042 kb on - strand, within Pf1N1B4_5360 at 96.042 kb on - strand, within Pf1N1B4_5360 at 96.042 kb on - strand, within Pf1N1B4_5360 at 96.042 kb on - strand, within Pf1N1B4_5360
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 94,086 + +0.4 94,086 + -2.6 94,096 + +2.3 94,172 + Pf1N1B4_5357 0.24 +0.3 94,172 + Pf1N1B4_5357 0.24 -1.2 94,173 - Pf1N1B4_5357 0.24 -0.7 94,333 + -0.7 94,334 - +0.9 94,344 + +0.6 94,345 - -0.8 94,361 + +1.0 94,386 + -1.6 94,387 - -1.6 94,528 - -1.6 94,530 + -0.0 94,530 + -1.2 94,530 + -1.0 94,530 + -1.3 94,530 + +0.7 94,530 + +1.0 94,531 - +0.4 94,531 - -0.0 94,532 + +1.0 94,532 + +0.3 94,532 + -1.0 94,532 + -2.0 94,532 + -0.6 94,532 + -1.8 94,532 + -0.8 94,532 + +0.2 94,533 - -2.0 94,600 - -0.8 94,662 + -2.0 94,662 + -0.0 94,662 + -1.0 94,662 + +1.0 94,662 + +0.4 94,662 + -1.6 94,662 + -1.0 94,662 + +2.0 94,663 - -0.6 94,663 - -2.6 94,707 + -1.0 94,752 + -2.3 94,752 + -2.3 94,752 + -0.0 94,752 + -1.0 94,752 + -0.0 94,752 + +1.6 94,752 + +0.7 94,752 + -0.0 94,753 - -2.8 94,753 - +0.6 94,825 + -0.0 94,825 + -1.6 94,825 + -0.0 94,825 + -2.3 94,825 + +0.6 94,862 + -0.7 94,863 - Pf1N1B4_5358 0.10 +3.2 94,939 - Pf1N1B4_5358 0.58 -0.0 94,996 + +0.4 95,073 + -1.3 95,073 + +0.5 95,079 + -0.6 95,079 + -1.6 95,080 - -0.0 95,080 - -0.6 95,145 + Pf1N1B4_5359 0.20 -1.6 95,145 + Pf1N1B4_5359 0.20 -1.0 95,160 + Pf1N1B4_5359 0.26 -2.8 95,213 + Pf1N1B4_5359 0.44 -1.2 95,213 + Pf1N1B4_5359 0.44 +1.0 95,213 + Pf1N1B4_5359 0.44 +1.0 95,213 + Pf1N1B4_5359 0.44 -0.0 95,214 - Pf1N1B4_5359 0.44 -2.0 95,371 + +1.7 95,372 - -1.0 95,430 + -2.3 95,620 + -0.0 95,620 + -0.4 95,734 - -0.0 95,807 + Pf1N1B4_5360 0.25 -1.0 95,807 + Pf1N1B4_5360 0.25 +1.6 95,808 - Pf1N1B4_5360 0.25 -2.6 95,808 - Pf1N1B4_5360 0.25 -1.2 95,808 - -0.0 95,808 - +1.6 95,808 - -0.0 95,808 - Pf1N1B4_5360 0.25 -1.6 95,808 - Pf1N1B4_5360 0.25 -1.5 95,808 - Pf1N1B4_5360 0.25 -2.0 95,808 - Pf1N1B4_5360 0.25 +1.0 95,808 - Pf1N1B4_5360 0.25 -1.8 95,808 - Pf1N1B4_5360 0.25 -0.0 95,834 + Pf1N1B4_5360 0.32 +0.7 95,835 - Pf1N1B4_5360 0.33 -0.0 95,835 - Pf1N1B4_5360 0.33 -0.0 95,912 + Pf1N1B4_5360 0.53 +0.7 95,912 + Pf1N1B4_5360 0.53 -0.0 95,912 + Pf1N1B4_5360 0.53 +0.1 95,913 - Pf1N1B4_5360 0.53 +1.0 95,913 - Pf1N1B4_5360 0.53 -0.3 95,913 - Pf1N1B4_5360 0.53 -1.0 96,042 - Pf1N1B4_5360 0.87 -3.0 96,042 - Pf1N1B4_5360 0.87 -1.0 96,042 - Pf1N1B4_5360 0.87 -0.4 96,042 - Pf1N1B4_5360 0.87 -1.0 96,042 - Pf1N1B4_5360 0.87 +0.1
Or see this region's nucleotide sequence