Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5251 and Pf1N1B4_5252 are separated by 169 nucleotides Pf1N1B4_5252 and Pf1N1B4_5253 are separated by 11 nucleotides Pf1N1B4_5253 and Pf1N1B4_5254 are separated by 277 nucleotides
Pf1N1B4_5251 - hypothetical protein, at 9,517 to 10,368
_5251
Pf1N1B4_5252 - hypothetical protein, at 10,538 to 11,272
_5252
Pf1N1B4_5253 - hypothetical protein, at 11,284 to 12,045
_5253
Pf1N1B4_5254 - hypothetical protein, at 12,323 to 13,168
_5254
Position (kb)
11
12
13 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 10.357 kb on - strand at 10.384 kb on + strand at 10.401 kb on - strand at 10.401 kb on - strand at 10.453 kb on - strand at 10.453 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.552 kb on - strand at 10.733 kb on + strand, within Pf1N1B4_5252 at 10.780 kb on - strand, within Pf1N1B4_5252 at 10.803 kb on + strand, within Pf1N1B4_5252 at 10.803 kb on + strand, within Pf1N1B4_5252 at 10.819 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.841 kb on - strand, within Pf1N1B4_5252 at 10.929 kb on + strand, within Pf1N1B4_5252 at 11.130 kb on + strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.134 kb on + strand, within Pf1N1B4_5252 at 11.134 kb on + strand, within Pf1N1B4_5252 at 11.159 kb on + strand, within Pf1N1B4_5252 at 11.205 kb on + strand at 11.206 kb on - strand at 11.206 kb on - strand at 11.206 kb on - strand at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.409 kb on - strand, within Pf1N1B4_5253 at 11.423 kb on + strand, within Pf1N1B4_5253 at 11.423 kb on + strand, within Pf1N1B4_5253 at 11.424 kb on - strand, within Pf1N1B4_5253 at 11.432 kb on + strand, within Pf1N1B4_5253 at 11.432 kb on + strand at 11.433 kb on - strand, within Pf1N1B4_5253 at 11.790 kb on - strand at 11.790 kb on - strand, within Pf1N1B4_5253 at 11.864 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.921 kb on - strand, within Pf1N1B4_5253 at 11.921 kb on - strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.925 kb on - strand, within Pf1N1B4_5253 at 11.925 kb on - strand, within Pf1N1B4_5253 at 11.969 kb on + strand at 11.969 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.034 kb on - strand at 12.034 kb on - strand at 12.034 kb on - strand at 12.068 kb on - strand at 12.068 kb on - strand at 12.153 kb on + strand at 12.418 kb on - strand, within Pf1N1B4_5254 at 12.485 kb on - strand, within Pf1N1B4_5254 at 12.485 kb on - strand, within Pf1N1B4_5254 at 12.485 kb on - strand, within Pf1N1B4_5254 at 12.546 kb on + strand, within Pf1N1B4_5254 at 12.547 kb on - strand at 12.547 kb on - strand, within Pf1N1B4_5254 at 12.549 kb on + strand, within Pf1N1B4_5254 at 12.549 kb on + strand, within Pf1N1B4_5254 at 12.549 kb on + strand, within Pf1N1B4_5254 at 12.741 kb on + strand, within Pf1N1B4_5254 at 12.859 kb on + strand, within Pf1N1B4_5254 at 12.860 kb on - strand at 12.860 kb on - strand, within Pf1N1B4_5254 at 12.952 kb on + strand, within Pf1N1B4_5254 at 12.953 kb on - strand, within Pf1N1B4_5254
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 10,357 - +0.2 10,384 + -2.6 10,401 - +0.3 10,401 - +1.2 10,453 - +1.5 10,453 - +0.3 10,496 - +0.5 10,496 - +1.1 10,496 - -1.3 10,496 - -0.0 10,500 - -1.0 10,500 - -0.0 10,500 - +1.0 10,500 - -1.6 10,552 - +1.2 10,733 + Pf1N1B4_5252 0.27 -0.2 10,780 - Pf1N1B4_5252 0.33 -0.3 10,803 + Pf1N1B4_5252 0.36 -1.6 10,803 + Pf1N1B4_5252 0.36 -0.4 10,819 + Pf1N1B4_5252 0.38 -1.3 10,821 + Pf1N1B4_5252 0.39 -0.8 10,821 + Pf1N1B4_5252 0.39 -2.6 10,821 + Pf1N1B4_5252 0.39 -0.3 10,822 - Pf1N1B4_5252 0.39 -0.0 10,822 - Pf1N1B4_5252 0.39 +0.2 10,822 - Pf1N1B4_5252 0.39 +0.8 10,841 - Pf1N1B4_5252 0.41 -0.0 10,929 + Pf1N1B4_5252 0.53 -0.0 11,130 + Pf1N1B4_5252 0.81 -2.0 11,131 - Pf1N1B4_5252 0.81 -0.4 11,131 - Pf1N1B4_5252 0.81 +0.6 11,131 - Pf1N1B4_5252 0.81 +0.9 11,134 + Pf1N1B4_5252 0.81 +1.6 11,134 + Pf1N1B4_5252 0.81 +0.3 11,159 + Pf1N1B4_5252 0.84 -0.1 11,205 + +0.5 11,206 - +1.2 11,206 - -0.6 11,206 - -1.4 11,408 + Pf1N1B4_5253 0.16 -0.2 11,408 + Pf1N1B4_5253 0.16 -0.0 11,408 + Pf1N1B4_5253 0.16 -0.4 11,408 + Pf1N1B4_5253 0.16 -0.4 11,409 - Pf1N1B4_5253 0.16 -0.0 11,423 + Pf1N1B4_5253 0.18 -1.3 11,423 + Pf1N1B4_5253 0.18 -0.6 11,424 - Pf1N1B4_5253 0.18 -2.0 11,432 + Pf1N1B4_5253 0.19 +1.8 11,432 + -1.6 11,433 - Pf1N1B4_5253 0.20 +0.7 11,790 - +2.0 11,790 - Pf1N1B4_5253 0.66 -0.0 11,864 + Pf1N1B4_5253 0.76 +1.4 11,920 + Pf1N1B4_5253 0.83 -0.0 11,920 + Pf1N1B4_5253 0.83 -1.6 11,920 + Pf1N1B4_5253 0.83 -1.2 11,920 + Pf1N1B4_5253 0.83 -1.6 11,921 - Pf1N1B4_5253 0.84 +2.0 11,921 - Pf1N1B4_5253 0.84 +0.3 11,924 + Pf1N1B4_5253 0.84 -0.3 11,924 + Pf1N1B4_5253 0.84 +2.6 11,924 + Pf1N1B4_5253 0.84 +0.1 11,925 - Pf1N1B4_5253 0.84 -0.6 11,925 - Pf1N1B4_5253 0.84 +1.3 11,969 + -1.6 11,969 + -0.6 12,033 + +0.6 12,033 + +0.6 12,033 + -1.4 12,033 + +0.6 12,034 - -1.9 12,034 - -0.4 12,034 - -1.0 12,068 - -2.6 12,068 - -0.3 12,153 + -1.0 12,418 - Pf1N1B4_5254 0.11 -0.6 12,485 - Pf1N1B4_5254 0.19 -0.6 12,485 - Pf1N1B4_5254 0.19 -1.0 12,485 - Pf1N1B4_5254 0.19 -1.3 12,546 + Pf1N1B4_5254 0.26 +0.2 12,547 - -1.0 12,547 - Pf1N1B4_5254 0.26 +0.8 12,549 + Pf1N1B4_5254 0.27 -1.0 12,549 + Pf1N1B4_5254 0.27 -1.6 12,549 + Pf1N1B4_5254 0.27 +1.0 12,741 + Pf1N1B4_5254 0.49 -0.0 12,859 + Pf1N1B4_5254 0.63 +2.6 12,860 - -0.6 12,860 - Pf1N1B4_5254 0.63 +1.2 12,952 + Pf1N1B4_5254 0.74 +1.2 12,953 - Pf1N1B4_5254 0.74 -0.4
Or see this region's nucleotide sequence