Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5107 and Pf1N1B4_5238 are separated by 202 nucleotides Pf1N1B4_5238 and Pf1N1B4_5108 are separated by 152 nucleotides Pf1N1B4_5108 and Pf1N1B4_5109 are separated by 244 nucleotides
Pf1N1B4_5107 - Putative collagenase, at 5,661,290 to 5,663,290
_5107
Pf1N1B4_5238 - tRNA-Gln-TTG, at 5,663,493 to 5,663,564
_5238
Pf1N1B4_5108 - Capsule biosynthesis protein capA, at 5,663,717 to 5,664,829
_5108
Pf1N1B4_5109 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 5,665,074 to 5,666,813
_5109
Position (kb)
5663
5664
5665 Strain fitness (log2 ratio)
-2
-1
0
1 at 5662.719 kb on - strand, within Pf1N1B4_5107 at 5662.940 kb on + strand, within Pf1N1B4_5107 at 5662.941 kb on - strand, within Pf1N1B4_5107 at 5663.467 kb on + strand at 5663.631 kb on + strand at 5663.820 kb on - strand at 5663.828 kb on + strand at 5663.828 kb on + strand at 5663.829 kb on - strand, within Pf1N1B4_5108 at 5663.829 kb on - strand, within Pf1N1B4_5108 at 5663.906 kb on + strand, within Pf1N1B4_5108 at 5663.907 kb on - strand, within Pf1N1B4_5108 at 5664.137 kb on - strand, within Pf1N1B4_5108 at 5664.493 kb on - strand, within Pf1N1B4_5108 at 5664.493 kb on - strand, within Pf1N1B4_5108 at 5664.636 kb on - strand, within Pf1N1B4_5108 at 5664.636 kb on - strand, within Pf1N1B4_5108 at 5664.809 kb on - strand at 5664.827 kb on + strand at 5664.828 kb on - strand at 5664.984 kb on - strand at 5665.356 kb on + strand, within Pf1N1B4_5109 at 5665.593 kb on - strand, within Pf1N1B4_5109 at 5665.687 kb on - strand, within Pf1N1B4_5109
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 5,662,719 - Pf1N1B4_5107 0.71 +1.2 5,662,940 + Pf1N1B4_5107 0.82 -1.6 5,662,941 - Pf1N1B4_5107 0.83 -2.2 5,663,467 + -0.2 5,663,631 + -1.4 5,663,820 - -0.1 5,663,828 + +0.4 5,663,828 + +0.9 5,663,829 - Pf1N1B4_5108 0.10 -1.4 5,663,829 - Pf1N1B4_5108 0.10 -1.6 5,663,906 + Pf1N1B4_5108 0.17 +0.9 5,663,907 - Pf1N1B4_5108 0.17 -0.4 5,664,137 - Pf1N1B4_5108 0.38 -1.2 5,664,493 - Pf1N1B4_5108 0.70 -0.1 5,664,493 - Pf1N1B4_5108 0.70 -1.3 5,664,636 - Pf1N1B4_5108 0.83 -0.8 5,664,636 - Pf1N1B4_5108 0.83 +0.6 5,664,809 - -0.9 5,664,827 + +1.0 5,664,828 - +0.7 5,664,984 - +0.4 5,665,356 + Pf1N1B4_5109 0.16 -0.3 5,665,593 - Pf1N1B4_5109 0.30 +1.7 5,665,687 - Pf1N1B4_5109 0.35 +1.2
Or see this region's nucleotide sequence