Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5062 and Pf1N1B4_5063 are separated by 36 nucleotides Pf1N1B4_5063 and Pf1N1B4_5064 are separated by 96 nucleotides
Pf1N1B4_5062 - Homospermidine synthase (EC 2.5.1.44), at 5,607,662 to 5,609,086
_5062
Pf1N1B4_5063 - Phosphoribosyl transferase domain protein, at 5,609,123 to 5,609,812
_5063
Pf1N1B4_5064 - Universal stress protein family 5, at 5,609,909 to 5,610,847
_5064
Position (kb)
5609
5610 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5608.431 kb on - strand, within Pf1N1B4_5062 at 5608.434 kb on - strand, within Pf1N1B4_5062 at 5608.463 kb on + strand, within Pf1N1B4_5062 at 5608.463 kb on + strand at 5608.464 kb on - strand at 5608.464 kb on - strand, within Pf1N1B4_5062 at 5608.572 kb on - strand, within Pf1N1B4_5062 at 5608.779 kb on - strand at 5608.785 kb on - strand, within Pf1N1B4_5062 at 5608.978 kb on - strand at 5608.978 kb on - strand at 5608.989 kb on - strand at 5608.989 kb on - strand at 5609.233 kb on - strand, within Pf1N1B4_5063 at 5609.474 kb on + strand, within Pf1N1B4_5063 at 5609.474 kb on + strand, within Pf1N1B4_5063 at 5609.475 kb on - strand, within Pf1N1B4_5063 at 5609.688 kb on + strand, within Pf1N1B4_5063 at 5609.689 kb on - strand, within Pf1N1B4_5063 at 5609.689 kb on - strand at 5609.744 kb on - strand at 5609.821 kb on + strand at 5609.948 kb on - strand at 5609.948 kb on - strand at 5610.376 kb on - strand, within Pf1N1B4_5064 at 5610.613 kb on - strand, within Pf1N1B4_5064 at 5610.662 kb on - strand, within Pf1N1B4_5064 at 5610.774 kb on - strand at 5610.776 kb on + strand at 5610.786 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 5,608,431 - Pf1N1B4_5062 0.54 +0.1 5,608,434 - Pf1N1B4_5062 0.54 -1.6 5,608,463 + Pf1N1B4_5062 0.56 -2.6 5,608,463 + -2.6 5,608,464 - -1.2 5,608,464 - Pf1N1B4_5062 0.56 -2.6 5,608,572 - Pf1N1B4_5062 0.64 -0.1 5,608,779 - +0.4 5,608,785 - Pf1N1B4_5062 0.79 -0.2 5,608,978 - +0.5 5,608,978 - +1.6 5,608,989 - +1.9 5,608,989 - -0.6 5,609,233 - Pf1N1B4_5063 0.16 +0.4 5,609,474 + Pf1N1B4_5063 0.51 -0.8 5,609,474 + Pf1N1B4_5063 0.51 +0.4 5,609,475 - Pf1N1B4_5063 0.51 -1.2 5,609,688 + Pf1N1B4_5063 0.82 -0.6 5,609,689 - Pf1N1B4_5063 0.82 -3.0 5,609,689 - -1.6 5,609,744 - -1.2 5,609,821 + -0.4 5,609,948 - -0.6 5,609,948 - -0.9 5,610,376 - Pf1N1B4_5064 0.50 +0.4 5,610,613 - Pf1N1B4_5064 0.75 -2.2 5,610,662 - Pf1N1B4_5064 0.80 -0.6 5,610,774 - -0.1 5,610,776 + +0.9 5,610,786 + -1.0
Or see this region's nucleotide sequence