Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_489 and Pf1N1B4_490 are separated by 466 nucleotides Pf1N1B4_490 and Pf1N1B4_491 are separated by 740 nucleotides
Pf1N1B4_489 - hypothetical protein possibly connected to lactam utilization and allophanate hydrolase, at 581,064 to 581,867
_489
Pf1N1B4_490 - hypothetical protein, at 582,334 to 582,507
_490
Pf1N1B4_491 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 583,248 to 584,471
_491
Position (kb) 582
583 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 581.355 kb on + strand, within Pf1N1B4_489 at 581.518 kb on - strand, within Pf1N1B4_489 at 581.518 kb on - strand, within Pf1N1B4_489 at 581.518 kb on - strand, within Pf1N1B4_489 at 581.520 kb on - strand, within Pf1N1B4_489 at 581.530 kb on + strand, within Pf1N1B4_489 at 581.530 kb on + strand at 581.530 kb on + strand, within Pf1N1B4_489 at 581.530 kb on + strand, within Pf1N1B4_489 at 581.531 kb on - strand at 581.531 kb on - strand, within Pf1N1B4_489 at 581.531 kb on - strand, within Pf1N1B4_489 at 581.531 kb on - strand, within Pf1N1B4_489 at 581.531 kb on - strand at 581.531 kb on - strand, within Pf1N1B4_489 at 581.785 kb on + strand, within Pf1N1B4_489 at 581.786 kb on - strand, within Pf1N1B4_489 at 581.894 kb on - strand at 581.966 kb on + strand at 581.966 kb on + strand at 581.980 kb on + strand at 581.981 kb on - strand at 581.981 kb on - strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 582.072 kb on + strand at 582.073 kb on - strand at 582.120 kb on - strand at 582.120 kb on - strand at 582.158 kb on - strand at 582.158 kb on - strand at 582.159 kb on + strand at 582.159 kb on + strand at 582.160 kb on - strand at 582.160 kb on - strand at 582.160 kb on - strand at 582.160 kb on - strand at 582.271 kb on + strand at 582.271 kb on + strand at 582.272 kb on - strand at 582.319 kb on - strand at 582.319 kb on - strand at 582.440 kb on + strand, within Pf1N1B4_490 at 582.440 kb on + strand, within Pf1N1B4_490 at 582.440 kb on + strand, within Pf1N1B4_490 at 582.441 kb on - strand, within Pf1N1B4_490 at 582.441 kb on - strand, within Pf1N1B4_490 at 582.466 kb on + strand, within Pf1N1B4_490 at 582.466 kb on + strand, within Pf1N1B4_490 at 582.480 kb on + strand, within Pf1N1B4_490 at 582.480 kb on + strand, within Pf1N1B4_490 at 582.505 kb on + strand at 582.505 kb on + strand at 582.505 kb on + strand at 582.506 kb on - strand at 582.506 kb on - strand at 582.506 kb on - strand at 582.511 kb on + strand at 582.512 kb on - strand at 582.512 kb on - strand at 582.525 kb on + strand at 582.525 kb on + strand at 582.537 kb on + strand at 582.537 kb on + strand at 582.537 kb on + strand at 582.537 kb on + strand at 582.538 kb on - strand at 582.538 kb on - strand at 582.538 kb on - strand at 582.564 kb on + strand at 582.565 kb on - strand at 582.588 kb on + strand at 582.588 kb on + strand at 582.640 kb on + strand at 582.640 kb on + strand at 582.641 kb on - strand at 582.641 kb on - strand at 582.641 kb on - strand at 582.691 kb on - strand at 582.811 kb on + strand at 582.812 kb on - strand at 582.812 kb on - strand at 582.835 kb on + strand at 582.894 kb on + strand at 582.895 kb on - strand at 582.895 kb on - strand at 582.895 kb on - strand at 582.902 kb on + strand at 582.903 kb on - strand at 582.989 kb on - strand at 583.011 kb on + strand at 583.011 kb on + strand at 583.011 kb on + strand at 583.011 kb on + strand at 583.012 kb on - strand at 583.012 kb on - strand at 583.013 kb on + strand at 583.049 kb on + strand at 583.061 kb on + strand at 583.061 kb on + strand at 583.061 kb on + strand at 583.062 kb on - strand at 583.129 kb on - strand at 583.187 kb on + strand at 583.187 kb on + strand at 583.188 kb on - strand at 583.188 kb on - strand at 583.188 kb on - strand at 583.188 kb on - strand at 583.188 kb on - strand at 583.188 kb on - strand at 583.246 kb on + strand at 583.247 kb on - strand at 583.489 kb on + strand, within Pf1N1B4_491 at 583.489 kb on + strand, within Pf1N1B4_491 at 583.490 kb on - strand, within Pf1N1B4_491 at 583.490 kb on - strand, within Pf1N1B4_491 at 583.490 kb on - strand, within Pf1N1B4_491
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 581,355 + Pf1N1B4_489 0.36 -1.0 581,518 - Pf1N1B4_489 0.56 -0.4 581,518 - Pf1N1B4_489 0.56 +1.0 581,518 - Pf1N1B4_489 0.56 -0.3 581,520 - Pf1N1B4_489 0.57 +1.3 581,530 + Pf1N1B4_489 0.58 +1.5 581,530 + -1.0 581,530 + Pf1N1B4_489 0.58 +0.6 581,530 + Pf1N1B4_489 0.58 -3.0 581,531 - +1.6 581,531 - Pf1N1B4_489 0.58 -1.0 581,531 - Pf1N1B4_489 0.58 -0.6 581,531 - Pf1N1B4_489 0.58 +0.8 581,531 - -1.6 581,531 - Pf1N1B4_489 0.58 -0.6 581,785 + Pf1N1B4_489 0.90 -1.4 581,786 - Pf1N1B4_489 0.90 +0.4 581,894 - +0.3 581,966 + +0.3 581,966 + +1.0 581,980 + +1.8 581,981 - +2.3 581,981 - +0.0 581,984 + +0.9 581,984 + +0.3 581,984 + +0.0 581,984 + +1.4 581,984 + +1.0 581,985 - +0.0 581,985 - +1.0 581,985 - +0.0 581,985 - +0.0 581,985 - +0.0 581,985 - +0.5 581,985 - -1.6 582,072 + +2.6 582,073 - -1.6 582,120 - +0.0 582,120 - -0.6 582,158 - -1.6 582,158 - +0.6 582,159 + -0.4 582,159 + +2.2 582,160 - +0.7 582,160 - -1.0 582,160 - -0.7 582,160 - +0.0 582,271 + +2.0 582,271 + +1.2 582,272 - +0.1 582,319 - +0.0 582,319 - +0.5 582,440 + Pf1N1B4_490 0.61 +2.6 582,440 + Pf1N1B4_490 0.61 +0.0 582,440 + Pf1N1B4_490 0.61 +0.7 582,441 - Pf1N1B4_490 0.61 +2.0 582,441 - Pf1N1B4_490 0.61 +0.3 582,466 + Pf1N1B4_490 0.76 +0.3 582,466 + Pf1N1B4_490 0.76 +1.7 582,480 + Pf1N1B4_490 0.84 +0.0 582,480 + Pf1N1B4_490 0.84 -1.0 582,505 + -3.3 582,505 + +0.0 582,505 + -1.6 582,506 - +1.3 582,506 - -1.6 582,506 - +0.6 582,511 + -1.6 582,512 - -1.4 582,512 - -0.8 582,525 + +0.5 582,525 + +1.0 582,537 + -2.0 582,537 + +1.0 582,537 + -1.6 582,537 + +0.6 582,538 - +0.0 582,538 - +0.2 582,538 - +0.0 582,564 + +1.3 582,565 - -0.4 582,588 + +1.6 582,588 + -1.4 582,640 + +1.2 582,640 + -1.6 582,641 - -2.6 582,641 - +0.1 582,641 - -0.7 582,691 - +2.1 582,811 + -0.6 582,812 - -1.3 582,812 - -1.0 582,835 + +1.6 582,894 + -0.5 582,895 - +0.0 582,895 - -2.0 582,895 - -1.6 582,902 + +1.0 582,903 - -1.0 582,989 - +0.4 583,011 + -1.0 583,011 + -1.6 583,011 + -2.6 583,011 + +1.6 583,012 - +0.8 583,012 - +0.9 583,013 + +0.0 583,049 + +0.4 583,061 + +1.3 583,061 + -1.3 583,061 + -2.0 583,062 - +2.0 583,129 - +2.0 583,187 + -1.0 583,187 + -0.6 583,188 - +0.5 583,188 - -1.0 583,188 - +1.1 583,188 - -0.6 583,188 - +0.0 583,188 - +0.0 583,246 + +0.0 583,247 - +0.0 583,489 + Pf1N1B4_491 0.20 +1.8 583,489 + Pf1N1B4_491 0.20 +0.0 583,490 - Pf1N1B4_491 0.20 +1.0 583,490 - Pf1N1B4_491 0.20 -0.5 583,490 - Pf1N1B4_491 0.20 +0.7
Or see this region's nucleotide sequence