Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4889 and Pf1N1B4_4890 overlap by 4 nucleotides Pf1N1B4_4890 and Pf1N1B4_4891 are separated by 122 nucleotides Pf1N1B4_4891 and Pf1N1B4_4892 are separated by 277 nucleotides
Pf1N1B4_4889 - Cystathionine beta-synthase (EC 4.2.1.22), at 5,411,076 to 5,412,452
_4889
Pf1N1B4_4890 - Cystathionine gamma-lyase (EC 4.4.1.1), at 5,412,449 to 5,413,630
_4890
Pf1N1B4_4891 - FIG00959288: hypothetical protein, at 5,413,753 to 5,414,028
_4891
Pf1N1B4_4892 - Transcriptional regulator, AraC family, at 5,414,306 to 5,415,121
_4892
Position (kb)
5412
5413
5414 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5411.457 kb on + strand, within Pf1N1B4_4889 at 5411.458 kb on - strand, within Pf1N1B4_4889 at 5411.461 kb on - strand, within Pf1N1B4_4889 at 5411.558 kb on + strand, within Pf1N1B4_4889 at 5411.665 kb on + strand, within Pf1N1B4_4889 at 5411.665 kb on + strand, within Pf1N1B4_4889 at 5411.666 kb on - strand, within Pf1N1B4_4889 at 5411.721 kb on + strand, within Pf1N1B4_4889 at 5411.721 kb on + strand, within Pf1N1B4_4889 at 5411.909 kb on + strand, within Pf1N1B4_4889 at 5411.909 kb on + strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.015 kb on - strand, within Pf1N1B4_4889 at 5412.407 kb on + strand at 5412.569 kb on + strand, within Pf1N1B4_4890 at 5412.569 kb on + strand, within Pf1N1B4_4890 at 5412.570 kb on - strand, within Pf1N1B4_4890 at 5412.955 kb on + strand, within Pf1N1B4_4890 at 5412.955 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand at 5413.067 kb on + strand at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.068 kb on - strand, within Pf1N1B4_4890 at 5413.068 kb on - strand, within Pf1N1B4_4890 at 5413.302 kb on - strand, within Pf1N1B4_4890 at 5413.761 kb on - strand at 5414.306 kb on - strand at 5414.432 kb on + strand at 5414.432 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.540 kb on - strand, within Pf1N1B4_4892
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 5,411,457 + Pf1N1B4_4889 0.28 -0.5 5,411,458 - Pf1N1B4_4889 0.28 -1.7 5,411,461 - Pf1N1B4_4889 0.28 +0.8 5,411,558 + Pf1N1B4_4889 0.35 +1.3 5,411,665 + Pf1N1B4_4889 0.43 +0.7 5,411,665 + Pf1N1B4_4889 0.43 +0.1 5,411,666 - Pf1N1B4_4889 0.43 -0.7 5,411,721 + Pf1N1B4_4889 0.47 +0.3 5,411,721 + Pf1N1B4_4889 0.47 -0.1 5,411,909 + Pf1N1B4_4889 0.60 +0.2 5,411,909 + Pf1N1B4_4889 0.60 +0.8 5,412,013 - Pf1N1B4_4889 0.68 +0.9 5,412,013 - Pf1N1B4_4889 0.68 -1.1 5,412,013 - Pf1N1B4_4889 0.68 -1.7 5,412,013 - Pf1N1B4_4889 0.68 +0.3 5,412,015 - Pf1N1B4_4889 0.68 -0.7 5,412,407 + +0.6 5,412,569 + Pf1N1B4_4890 0.10 +0.7 5,412,569 + Pf1N1B4_4890 0.10 -0.7 5,412,570 - Pf1N1B4_4890 0.10 +0.3 5,412,955 + Pf1N1B4_4890 0.43 -2.7 5,412,955 + Pf1N1B4_4890 0.43 -2.7 5,413,067 + -1.7 5,413,067 + -0.7 5,413,067 + Pf1N1B4_4890 0.52 -0.5 5,413,067 + Pf1N1B4_4890 0.52 -0.3 5,413,067 + Pf1N1B4_4890 0.52 +0.2 5,413,068 - Pf1N1B4_4890 0.52 +0.9 5,413,068 - Pf1N1B4_4890 0.52 -0.7 5,413,302 - Pf1N1B4_4890 0.72 -1.7 5,413,761 - +0.1 5,414,306 - +1.1 5,414,432 + +0.9 5,414,432 + Pf1N1B4_4892 0.15 -0.3 5,414,483 + Pf1N1B4_4892 0.22 +1.9 5,414,483 + Pf1N1B4_4892 0.22 -1.3 5,414,540 - Pf1N1B4_4892 0.29 -3.0
Or see this region's nucleotide sequence