Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4725

Experiment: Fusidic 0.0625 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4723 and Pf1N1B4_4724 are separated by 198 nucleotidesPf1N1B4_4724 and Pf1N1B4_4725 are separated by 272 nucleotidesPf1N1B4_4725 and Pf1N1B4_4726 overlap by 4 nucleotides Pf1N1B4_4723 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 5,218,782 to 5,220,527 _4723 Pf1N1B4_4724 - FIG00961385: hypothetical protein, at 5,220,726 to 5,220,908 _4724 Pf1N1B4_4725 - 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44), at 5,221,181 to 5,222,161 _4725 Pf1N1B4_4726 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 5,222,158 to 5,223,681 _4726 Position (kb) 5221 5222 5223Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5220.405 kb on - strandat 5220.550 kb on - strandat 5220.550 kb on - strandat 5220.550 kb on - strandat 5220.550 kb on - strandat 5220.550 kb on - strandat 5220.550 kb on - strandat 5220.637 kb on - strandat 5220.637 kb on - strandat 5220.637 kb on - strandat 5220.898 kb on - strandat 5220.940 kb on - strandat 5220.970 kb on - strandat 5221.137 kb on + strandat 5221.137 kb on + strandat 5221.137 kb on + strandat 5221.157 kb on - strandat 5221.162 kb on + strandat 5221.166 kb on - strandat 5221.178 kb on + strandat 5221.178 kb on + strandat 5221.178 kb on + strandat 5221.178 kb on + strandat 5221.179 kb on - strandat 5221.179 kb on - strandat 5221.222 kb on + strandat 5221.252 kb on + strandat 5221.253 kb on - strandat 5221.265 kb on + strandat 5221.423 kb on + strand, within Pf1N1B4_4725at 5221.423 kb on + strand, within Pf1N1B4_4725at 5221.424 kb on - strand, within Pf1N1B4_4725at 5221.472 kb on + strand, within Pf1N1B4_4725at 5221.472 kb on + strand, within Pf1N1B4_4725at 5221.472 kb on + strand, within Pf1N1B4_4725at 5221.473 kb on - strandat 5221.473 kb on - strand, within Pf1N1B4_4725at 5221.475 kb on - strand, within Pf1N1B4_4725at 5221.572 kb on - strand, within Pf1N1B4_4725at 5222.269 kb on + strandat 5222.269 kb on + strandat 5222.270 kb on - strandat 5222.270 kb on - strandat 5222.270 kb on - strandat 5222.462 kb on - strand, within Pf1N1B4_4726at 5222.462 kb on - strand, within Pf1N1B4_4726at 5222.548 kb on + strand, within Pf1N1B4_4726at 5222.746 kb on + strand, within Pf1N1B4_4726at 5222.747 kb on - strand, within Pf1N1B4_4726at 5222.943 kb on - strand, within Pf1N1B4_4726at 5223.160 kb on + strand, within Pf1N1B4_4726at 5223.160 kb on + strand, within Pf1N1B4_4726at 5223.161 kb on - strand, within Pf1N1B4_4726

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml
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5,220,405 - -1.4
5,220,550 - -0.7
5,220,550 - +0.6
5,220,550 - +0.6
5,220,550 - -0.1
5,220,550 - -0.3
5,220,550 - +0.3
5,220,637 - +0.9
5,220,637 - +0.4
5,220,637 - -0.7
5,220,898 - -1.7
5,220,940 - +1.5
5,220,970 - -0.7
5,221,137 + +0.3
5,221,137 + +1.1
5,221,137 + -1.7
5,221,157 - +0.3
5,221,162 + -0.7
5,221,166 - +0.1
5,221,178 + -1.7
5,221,178 + +0.1
5,221,178 + +1.3
5,221,178 + -0.3
5,221,179 - +2.3
5,221,179 - +0.3
5,221,222 + +1.2
5,221,252 + -0.7
5,221,253 - -0.1
5,221,265 + +0.3
5,221,423 + Pf1N1B4_4725 0.25 -1.2
5,221,423 + Pf1N1B4_4725 0.25 -0.7
5,221,424 - Pf1N1B4_4725 0.25 -0.9
5,221,472 + Pf1N1B4_4725 0.30 +1.7
5,221,472 + Pf1N1B4_4725 0.30 -0.7
5,221,472 + Pf1N1B4_4725 0.30 +0.3
5,221,473 - +0.3
5,221,473 - Pf1N1B4_4725 0.30 +3.6
5,221,475 - Pf1N1B4_4725 0.30 +0.5
5,221,572 - Pf1N1B4_4725 0.40 +0.8
5,222,269 + +0.3
5,222,269 + +0.1
5,222,270 - +0.6
5,222,270 - -0.7
5,222,270 - +0.2
5,222,462 - Pf1N1B4_4726 0.20 +0.2
5,222,462 - Pf1N1B4_4726 0.20 +0.5
5,222,548 + Pf1N1B4_4726 0.26 +1.9
5,222,746 + Pf1N1B4_4726 0.39 +0.5
5,222,747 - Pf1N1B4_4726 0.39 -3.0
5,222,943 - Pf1N1B4_4726 0.52 -0.1
5,223,160 + Pf1N1B4_4726 0.66 +0.2
5,223,160 + Pf1N1B4_4726 0.66 -2.7
5,223,161 - Pf1N1B4_4726 0.66 -2.2

Or see this region's nucleotide sequence