Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4339 and Pf1N1B4_4340 are separated by 37 nucleotides Pf1N1B4_4340 and Pf1N1B4_4341 are separated by 103 nucleotides Pf1N1B4_4341 and Pf1N1B4_4342 are separated by 364 nucleotides Pf1N1B4_4342 and Pf1N1B4_4343 are separated by 27 nucleotides Pf1N1B4_4343 and Pf1N1B4_4344 overlap by 4 nucleotides
Pf1N1B4_4339 - Malic enzyme, at 4,798,331 to 4,799,551
_4339
Pf1N1B4_4340 - FOG: WD40-like repeat, at 4,799,589 to 4,800,218
_4340
Pf1N1B4_4341 - hypothetical protein, at 4,800,322 to 4,801,020
_4341
Pf1N1B4_4342 - hypothetical protein, at 4,801,385 to 4,801,528
_4342
Pf1N1B4_4343 - hypothetical protein, at 4,801,556 to 4,801,825
_4343
Pf1N1B4_4344 - hypothetical protein, at 4,801,822 to 4,801,977
_4344
Position (kb) 4800
4801
4802 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4799.517 kb on + strand at 4799.518 kb on - strand at 4799.524 kb on - strand at 4799.550 kb on - strand at 4799.622 kb on + strand at 4799.845 kb on + strand at 4799.845 kb on + strand, within Pf1N1B4_4340 at 4800.031 kb on + strand, within Pf1N1B4_4340 at 4800.032 kb on - strand, within Pf1N1B4_4340 at 4800.453 kb on + strand, within Pf1N1B4_4341 at 4800.454 kb on - strand at 4800.578 kb on + strand, within Pf1N1B4_4341 at 4800.610 kb on + strand, within Pf1N1B4_4341 at 4800.611 kb on - strand, within Pf1N1B4_4341 at 4800.616 kb on + strand, within Pf1N1B4_4341 at 4800.616 kb on + strand, within Pf1N1B4_4341 at 4800.616 kb on + strand, within Pf1N1B4_4341 at 4800.616 kb on + strand, within Pf1N1B4_4341 at 4800.631 kb on + strand at 4800.632 kb on - strand, within Pf1N1B4_4341 at 4800.701 kb on - strand, within Pf1N1B4_4341 at 4800.724 kb on + strand at 4800.724 kb on + strand, within Pf1N1B4_4341 at 4800.725 kb on - strand, within Pf1N1B4_4341 at 4801.228 kb on + strand at 4801.238 kb on + strand at 4801.238 kb on + strand at 4801.274 kb on + strand at 4801.343 kb on + strand at 4801.347 kb on + strand at 4801.348 kb on - strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.360 kb on - strand at 4801.360 kb on - strand at 4801.380 kb on + strand at 4801.396 kb on - strand at 4801.614 kb on - strand, within Pf1N1B4_4343 at 4801.770 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.774 kb on - strand, within Pf1N1B4_4343 at 4801.787 kb on + strand, within Pf1N1B4_4343 at 4801.955 kb on + strand, within Pf1N1B4_4344 at 4802.015 kb on + strand at 4802.015 kb on + strand at 4802.016 kb on - strand at 4802.019 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 4,799,517 + -0.7 4,799,518 - -0.4 4,799,524 - +0.1 4,799,550 - +0.3 4,799,622 + -0.9 4,799,845 + -0.4 4,799,845 + Pf1N1B4_4340 0.41 -0.4 4,800,031 + Pf1N1B4_4340 0.70 -1.4 4,800,032 - Pf1N1B4_4340 0.70 -0.4 4,800,453 + Pf1N1B4_4341 0.19 +1.6 4,800,454 - +0.6 4,800,578 + Pf1N1B4_4341 0.37 +1.2 4,800,610 + Pf1N1B4_4341 0.41 +1.9 4,800,611 - Pf1N1B4_4341 0.41 -2.0 4,800,616 + Pf1N1B4_4341 0.42 +1.0 4,800,616 + Pf1N1B4_4341 0.42 +0.2 4,800,616 + Pf1N1B4_4341 0.42 +0.2 4,800,616 + Pf1N1B4_4341 0.42 -1.0 4,800,631 + +1.6 4,800,632 - Pf1N1B4_4341 0.44 +0.4 4,800,701 - Pf1N1B4_4341 0.54 +2.4 4,800,724 + -0.4 4,800,724 + Pf1N1B4_4341 0.58 -0.4 4,800,725 - Pf1N1B4_4341 0.58 -0.8 4,801,228 + -1.2 4,801,238 + -0.8 4,801,238 + +0.6 4,801,274 + -0.4 4,801,343 + -2.0 4,801,347 + -1.0 4,801,348 - +0.3 4,801,359 + -1.4 4,801,359 + +3.8 4,801,359 + -2.4 4,801,359 + -0.4 4,801,359 + -1.4 4,801,360 - -0.4 4,801,360 - -2.8 4,801,380 + +0.2 4,801,396 - -1.4 4,801,614 - Pf1N1B4_4343 0.21 -0.0 4,801,770 + Pf1N1B4_4343 0.79 +2.2 4,801,773 + Pf1N1B4_4343 0.80 -1.4 4,801,773 + Pf1N1B4_4343 0.80 +0.6 4,801,773 + -1.4 4,801,773 + Pf1N1B4_4343 0.80 -0.4 4,801,773 + Pf1N1B4_4343 0.80 -0.2 4,801,773 + Pf1N1B4_4343 0.80 -2.8 4,801,774 - Pf1N1B4_4343 0.81 +0.6 4,801,787 + Pf1N1B4_4343 0.86 -0.4 4,801,955 + Pf1N1B4_4344 0.85 -0.7 4,802,015 + -1.4 4,802,015 + -0.5 4,802,016 - -0.7 4,802,019 + +1.2
Or see this region's nucleotide sequence