Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4062 and Pf1N1B4_4063 overlap by 1 nucleotides Pf1N1B4_4063 and Pf1N1B4_4064 are separated by 15 nucleotides Pf1N1B4_4064 and Pf1N1B4_4065 are separated by 0 nucleotides
Pf1N1B4_4062 - FIG065221: Holliday junction DNA helicase, at 4,516,297 to 4,517,619
_4062
Pf1N1B4_4063 - CrcB protein, at 4,517,619 to 4,517,993
_4063
Pf1N1B4_4064 - Seryl-tRNA synthetase (EC 6.1.1.11), at 4,518,009 to 4,519,289
_4064
Pf1N1B4_4065 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 4,519,290 to 4,520,684
_4065
Position (kb)
4518
4519
4520 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4517.041 kb on + strand, within Pf1N1B4_4062 at 4517.042 kb on - strand, within Pf1N1B4_4062 at 4517.042 kb on - strand, within Pf1N1B4_4062 at 4517.065 kb on - strand, within Pf1N1B4_4062 at 4517.143 kb on + strand, within Pf1N1B4_4062 at 4517.143 kb on + strand, within Pf1N1B4_4062 at 4517.144 kb on - strand, within Pf1N1B4_4062 at 4517.287 kb on - strand, within Pf1N1B4_4062 at 4517.290 kb on + strand, within Pf1N1B4_4062 at 4517.291 kb on - strand, within Pf1N1B4_4062 at 4517.291 kb on - strand, within Pf1N1B4_4062 at 4517.323 kb on + strand, within Pf1N1B4_4062 at 4517.324 kb on - strand, within Pf1N1B4_4062 at 4517.324 kb on - strand, within Pf1N1B4_4062 at 4517.488 kb on + strand at 4517.489 kb on - strand at 4517.489 kb on - strand at 4517.489 kb on - strand at 4517.594 kb on + strand at 4517.595 kb on - strand at 4517.721 kb on + strand, within Pf1N1B4_4063 at 4517.721 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.941 kb on + strand, within Pf1N1B4_4063 at 4519.287 kb on + strand at 4519.296 kb on + strand at 4519.296 kb on + strand at 4519.296 kb on + strand at 4519.297 kb on - strand at 4519.333 kb on + strand at 4519.333 kb on + strand at 4519.408 kb on + strand at 4519.408 kb on + strand at 4519.741 kb on - strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.029 kb on - strand, within Pf1N1B4_4065 at 4520.029 kb on - strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.204 kb on + strand, within Pf1N1B4_4065 at 4520.205 kb on - strand, within Pf1N1B4_4065
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 4,517,041 + Pf1N1B4_4062 0.56 -1.3 4,517,042 - Pf1N1B4_4062 0.56 -0.9 4,517,042 - Pf1N1B4_4062 0.56 +0.5 4,517,065 - Pf1N1B4_4062 0.58 +0.2 4,517,143 + Pf1N1B4_4062 0.64 -3.1 4,517,143 + Pf1N1B4_4062 0.64 +1.4 4,517,144 - Pf1N1B4_4062 0.64 -0.5 4,517,287 - Pf1N1B4_4062 0.75 +0.1 4,517,290 + Pf1N1B4_4062 0.75 -1.6 4,517,291 - Pf1N1B4_4062 0.75 -0.3 4,517,291 - Pf1N1B4_4062 0.75 +0.9 4,517,323 + Pf1N1B4_4062 0.78 +0.3 4,517,324 - Pf1N1B4_4062 0.78 +0.6 4,517,324 - Pf1N1B4_4062 0.78 +0.9 4,517,488 + -0.7 4,517,489 - +0.8 4,517,489 - +0.3 4,517,489 - +1.0 4,517,594 + -0.9 4,517,595 - +1.7 4,517,721 + Pf1N1B4_4063 0.27 -1.6 4,517,721 + Pf1N1B4_4063 0.27 +0.3 4,517,773 + Pf1N1B4_4063 0.41 +0.4 4,517,773 + Pf1N1B4_4063 0.41 -0.7 4,517,773 + Pf1N1B4_4063 0.41 -0.1 4,517,773 + Pf1N1B4_4063 0.41 -0.5 4,517,773 + Pf1N1B4_4063 0.41 +0.7 4,517,773 + Pf1N1B4_4063 0.41 +0.1 4,517,941 + Pf1N1B4_4063 0.86 +0.9 4,519,287 + +1.2 4,519,296 + -2.6 4,519,296 + -0.6 4,519,296 + -0.6 4,519,297 - +2.1 4,519,333 + +0.3 4,519,333 + -0.5 4,519,408 + -1.3 4,519,408 + +0.3 4,519,741 - Pf1N1B4_4065 0.32 -1.3 4,519,834 + Pf1N1B4_4065 0.39 -0.9 4,519,834 + Pf1N1B4_4065 0.39 +3.1 4,519,834 + -0.3 4,519,834 + Pf1N1B4_4065 0.39 -1.3 4,519,834 + Pf1N1B4_4065 0.39 -0.3 4,519,834 + Pf1N1B4_4065 0.39 -1.3 4,519,834 + Pf1N1B4_4065 0.39 -1.9 4,519,835 - Pf1N1B4_4065 0.39 -0.0 4,519,835 - Pf1N1B4_4065 0.39 +0.1 4,519,835 - Pf1N1B4_4065 0.39 +1.5 4,519,835 - Pf1N1B4_4065 0.39 -1.3 4,519,835 - Pf1N1B4_4065 0.39 -0.6 4,520,028 + Pf1N1B4_4065 0.53 -0.3 4,520,028 + Pf1N1B4_4065 0.53 -2.3 4,520,028 + Pf1N1B4_4065 0.53 -1.6 4,520,028 + Pf1N1B4_4065 0.53 +1.1 4,520,028 + Pf1N1B4_4065 0.53 -0.3 4,520,028 + Pf1N1B4_4065 0.53 +1.3 4,520,029 - Pf1N1B4_4065 0.53 -0.3 4,520,029 - Pf1N1B4_4065 0.53 -2.3 4,520,088 + Pf1N1B4_4065 0.57 -1.6 4,520,088 + Pf1N1B4_4065 0.57 -2.9 4,520,088 + Pf1N1B4_4065 0.57 -1.1 4,520,088 + Pf1N1B4_4065 0.57 -1.3 4,520,088 + Pf1N1B4_4065 0.57 -1.3 4,520,204 + Pf1N1B4_4065 0.66 +0.4 4,520,205 - Pf1N1B4_4065 0.66 +1.0
Or see this region's nucleotide sequence