Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3850 and Pf1N1B4_3851 are separated by 41 nucleotides Pf1N1B4_3851 and Pf1N1B4_3852 are separated by 127 nucleotides
Pf1N1B4_3850 - Guanine deaminase (EC 3.5.4.3), at 4,271,329 to 4,272,633
_3850
Pf1N1B4_3851 - XdhC protein (assists in molybdopterin insertion into xanthine dehydrogenase), at 4,272,675 to 4,273,529
_3851
Pf1N1B4_3852 - Xanthine dehydrogenase, molybdenum binding subunit (EC 1.17.1.4), at 4,273,657 to 4,276,053
_3852
Position (kb)
4272
4273
4274 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4271.853 kb on - strand, within Pf1N1B4_3850 at 4271.861 kb on + strand, within Pf1N1B4_3850 at 4271.862 kb on - strand, within Pf1N1B4_3850 at 4271.862 kb on - strand at 4271.948 kb on + strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4272.089 kb on + strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.117 kb on - strand at 4272.117 kb on - strand at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.366 kb on - strand, within Pf1N1B4_3850 at 4272.551 kb on + strand at 4272.647 kb on + strand at 4272.647 kb on + strand at 4272.648 kb on - strand at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.767 kb on - strand, within Pf1N1B4_3851 at 4272.767 kb on - strand, within Pf1N1B4_3851 at 4273.021 kb on + strand, within Pf1N1B4_3851 at 4273.021 kb on + strand, within Pf1N1B4_3851 at 4273.022 kb on - strand, within Pf1N1B4_3851 at 4273.354 kb on + strand, within Pf1N1B4_3851 at 4273.354 kb on + strand, within Pf1N1B4_3851 at 4273.354 kb on + strand, within Pf1N1B4_3851 at 4273.355 kb on - strand, within Pf1N1B4_3851 at 4273.355 kb on - strand, within Pf1N1B4_3851 at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.952 kb on + strand, within Pf1N1B4_3852 at 4273.953 kb on - strand, within Pf1N1B4_3852 at 4273.953 kb on - strand, within Pf1N1B4_3852 at 4274.120 kb on + strand, within Pf1N1B4_3852 at 4274.175 kb on - strand, within Pf1N1B4_3852 at 4274.177 kb on + strand, within Pf1N1B4_3852 at 4274.178 kb on - strand at 4274.178 kb on - strand at 4274.178 kb on - strand at 4274.178 kb on - strand, within Pf1N1B4_3852 at 4274.178 kb on - strand, within Pf1N1B4_3852 at 4274.180 kb on + strand, within Pf1N1B4_3852 at 4274.181 kb on - strand, within Pf1N1B4_3852 at 4274.181 kb on - strand, within Pf1N1B4_3852 at 4274.214 kb on - strand, within Pf1N1B4_3852 at 4274.214 kb on - strand, within Pf1N1B4_3852 at 4274.270 kb on + strand, within Pf1N1B4_3852 at 4274.363 kb on + strand, within Pf1N1B4_3852
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 4,271,853 - Pf1N1B4_3850 0.40 -0.5 4,271,861 + Pf1N1B4_3850 0.41 +0.2 4,271,862 - Pf1N1B4_3850 0.41 -1.8 4,271,862 - -2.2 4,271,948 + Pf1N1B4_3850 0.47 +0.8 4,271,949 - Pf1N1B4_3850 0.48 -0.9 4,271,949 - Pf1N1B4_3850 0.48 -0.2 4,271,949 - Pf1N1B4_3850 0.48 -1.2 4,271,949 - Pf1N1B4_3850 0.48 -0.8 4,271,949 - Pf1N1B4_3850 0.48 +0.6 4,272,089 + Pf1N1B4_3850 0.58 -2.5 4,272,090 - Pf1N1B4_3850 0.58 +1.0 4,272,090 - Pf1N1B4_3850 0.58 +0.4 4,272,090 - Pf1N1B4_3850 0.58 -1.2 4,272,117 - -0.2 4,272,117 - -1.8 4,272,332 + Pf1N1B4_3850 0.77 -1.5 4,272,332 + Pf1N1B4_3850 0.77 +0.8 4,272,332 + Pf1N1B4_3850 0.77 +2.4 4,272,332 + Pf1N1B4_3850 0.77 +1.8 4,272,332 + Pf1N1B4_3850 0.77 +0.4 4,272,333 - Pf1N1B4_3850 0.77 -0.2 4,272,333 - Pf1N1B4_3850 0.77 -0.2 4,272,333 - Pf1N1B4_3850 0.77 -1.6 4,272,333 - Pf1N1B4_3850 0.77 -0.2 4,272,333 - Pf1N1B4_3850 0.77 -1.5 4,272,333 - -1.2 4,272,333 - Pf1N1B4_3850 0.77 +1.4 4,272,352 + Pf1N1B4_3850 0.78 -1.1 4,272,352 + Pf1N1B4_3850 0.78 +1.1 4,272,352 + Pf1N1B4_3850 0.78 -3.2 4,272,352 + Pf1N1B4_3850 0.78 -1.8 4,272,366 - Pf1N1B4_3850 0.79 -0.8 4,272,551 + +0.8 4,272,647 + +0.2 4,272,647 + -0.2 4,272,648 - -0.6 4,272,766 + Pf1N1B4_3851 0.11 -1.2 4,272,766 + Pf1N1B4_3851 0.11 +0.4 4,272,766 + -0.8 4,272,766 + Pf1N1B4_3851 0.11 -0.4 4,272,766 + Pf1N1B4_3851 0.11 +0.8 4,272,766 + Pf1N1B4_3851 0.11 -0.2 4,272,766 + Pf1N1B4_3851 0.11 +1.4 4,272,766 + Pf1N1B4_3851 0.11 -1.8 4,272,767 - Pf1N1B4_3851 0.11 -0.2 4,272,767 - Pf1N1B4_3851 0.11 +0.2 4,273,021 + Pf1N1B4_3851 0.40 -0.6 4,273,021 + Pf1N1B4_3851 0.40 -0.2 4,273,022 - Pf1N1B4_3851 0.41 -1.2 4,273,354 + Pf1N1B4_3851 0.79 -0.2 4,273,354 + Pf1N1B4_3851 0.79 -0.2 4,273,354 + Pf1N1B4_3851 0.79 +0.8 4,273,355 - Pf1N1B4_3851 0.80 +0.8 4,273,355 - Pf1N1B4_3851 0.80 -0.7 4,273,516 + +1.5 4,273,516 + -1.2 4,273,516 + -1.4 4,273,516 + +0.4 4,273,516 + +0.7 4,273,516 + +0.8 4,273,517 - -0.9 4,273,517 - +1.0 4,273,517 - +1.0 4,273,517 - -0.2 4,273,517 - -0.4 4,273,517 - +0.4 4,273,517 - +0.5 4,273,517 - +0.9 4,273,517 - +0.4 4,273,952 + Pf1N1B4_3852 0.12 -0.8 4,273,953 - Pf1N1B4_3852 0.12 +1.0 4,273,953 - Pf1N1B4_3852 0.12 -1.0 4,274,120 + Pf1N1B4_3852 0.19 -0.2 4,274,175 - Pf1N1B4_3852 0.22 +0.7 4,274,177 + Pf1N1B4_3852 0.22 +0.5 4,274,178 - -0.2 4,274,178 - +1.4 4,274,178 - +2.1 4,274,178 - Pf1N1B4_3852 0.22 -1.2 4,274,178 - Pf1N1B4_3852 0.22 -2.8 4,274,180 + Pf1N1B4_3852 0.22 +3.3 4,274,181 - Pf1N1B4_3852 0.22 -0.2 4,274,181 - Pf1N1B4_3852 0.22 -1.8 4,274,214 - Pf1N1B4_3852 0.23 -1.5 4,274,214 - Pf1N1B4_3852 0.23 +1.6 4,274,270 + Pf1N1B4_3852 0.26 -1.2 4,274,363 + Pf1N1B4_3852 0.29 +0.1
Or see this region's nucleotide sequence