Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3849 and Pf1N1B4_3850 are separated by 179 nucleotides Pf1N1B4_3850 and Pf1N1B4_3851 are separated by 41 nucleotides
Pf1N1B4_3849 - Transcriptional regulator, GntR family, at 4,270,385 to 4,271,149
_3849
Pf1N1B4_3850 - Guanine deaminase (EC 3.5.4.3), at 4,271,329 to 4,272,633
_3850
Pf1N1B4_3851 - XdhC protein (assists in molybdopterin insertion into xanthine dehydrogenase), at 4,272,675 to 4,273,529
_3851
Position (kb)
4271
4272
4273 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4270.337 kb on + strand at 4270.337 kb on + strand at 4270.338 kb on - strand at 4270.338 kb on - strand at 4270.340 kb on + strand at 4270.340 kb on + strand at 4270.340 kb on + strand at 4270.340 kb on + strand at 4270.340 kb on + strand at 4270.340 kb on + strand at 4270.341 kb on - strand at 4270.341 kb on - strand at 4270.341 kb on - strand at 4270.341 kb on - strand at 4270.341 kb on - strand at 4270.459 kb on + strand at 4270.459 kb on + strand at 4270.459 kb on + strand at 4270.467 kb on - strand, within Pf1N1B4_3849 at 4270.467 kb on - strand, within Pf1N1B4_3849 at 4270.474 kb on + strand, within Pf1N1B4_3849 at 4270.474 kb on + strand, within Pf1N1B4_3849 at 4270.474 kb on + strand at 4270.474 kb on + strand, within Pf1N1B4_3849 at 4270.475 kb on - strand, within Pf1N1B4_3849 at 4270.475 kb on - strand, within Pf1N1B4_3849 at 4270.922 kb on + strand, within Pf1N1B4_3849 at 4270.922 kb on + strand, within Pf1N1B4_3849 at 4270.922 kb on + strand, within Pf1N1B4_3849 at 4270.952 kb on + strand, within Pf1N1B4_3849 at 4270.952 kb on + strand, within Pf1N1B4_3849 at 4270.953 kb on - strand, within Pf1N1B4_3849 at 4270.953 kb on - strand, within Pf1N1B4_3849 at 4271.360 kb on + strand at 4271.360 kb on + strand at 4271.360 kb on + strand at 4271.361 kb on - strand at 4271.361 kb on - strand at 4271.361 kb on - strand at 4271.361 kb on - strand at 4271.401 kb on + strand at 4271.401 kb on + strand at 4271.402 kb on - strand at 4271.402 kb on - strand at 4271.402 kb on - strand at 4271.402 kb on - strand at 4271.444 kb on + strand at 4271.469 kb on - strand, within Pf1N1B4_3850 at 4271.533 kb on + strand, within Pf1N1B4_3850 at 4271.533 kb on + strand, within Pf1N1B4_3850 at 4271.534 kb on - strand, within Pf1N1B4_3850 at 4271.564 kb on + strand, within Pf1N1B4_3850 at 4271.600 kb on - strand, within Pf1N1B4_3850 at 4271.600 kb on - strand, within Pf1N1B4_3850 at 4271.600 kb on - strand, within Pf1N1B4_3850 at 4271.615 kb on + strand, within Pf1N1B4_3850 at 4271.616 kb on - strand, within Pf1N1B4_3850 at 4271.616 kb on - strand at 4271.853 kb on - strand, within Pf1N1B4_3850 at 4271.861 kb on + strand, within Pf1N1B4_3850 at 4271.862 kb on - strand, within Pf1N1B4_3850 at 4271.862 kb on - strand at 4271.948 kb on + strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4272.089 kb on + strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.117 kb on - strand at 4272.117 kb on - strand at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.366 kb on - strand, within Pf1N1B4_3850 at 4272.551 kb on + strand at 4272.647 kb on + strand at 4272.647 kb on + strand at 4272.648 kb on - strand at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.767 kb on - strand, within Pf1N1B4_3851 at 4272.767 kb on - strand, within Pf1N1B4_3851 at 4273.021 kb on + strand, within Pf1N1B4_3851 at 4273.021 kb on + strand, within Pf1N1B4_3851 at 4273.022 kb on - strand, within Pf1N1B4_3851 at 4273.354 kb on + strand, within Pf1N1B4_3851 at 4273.354 kb on + strand, within Pf1N1B4_3851 at 4273.354 kb on + strand, within Pf1N1B4_3851 at 4273.355 kb on - strand, within Pf1N1B4_3851 at 4273.355 kb on - strand, within Pf1N1B4_3851 at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 4,270,337 + +1.1 4,270,337 + -1.2 4,270,338 - +0.2 4,270,338 - +1.1 4,270,340 + -1.2 4,270,340 + -1.2 4,270,340 + -0.8 4,270,340 + -1.0 4,270,340 + -0.2 4,270,340 + -0.7 4,270,341 - -2.8 4,270,341 - +0.2 4,270,341 - -1.0 4,270,341 - +0.8 4,270,341 - -0.3 4,270,459 + +1.0 4,270,459 + +0.8 4,270,459 + +2.1 4,270,467 - Pf1N1B4_3849 0.11 -1.2 4,270,467 - Pf1N1B4_3849 0.11 +0.5 4,270,474 + Pf1N1B4_3849 0.12 +1.9 4,270,474 + Pf1N1B4_3849 0.12 -0.9 4,270,474 + -1.8 4,270,474 + Pf1N1B4_3849 0.12 +0.8 4,270,475 - Pf1N1B4_3849 0.12 +2.1 4,270,475 - Pf1N1B4_3849 0.12 +0.1 4,270,922 + Pf1N1B4_3849 0.70 -0.2 4,270,922 + Pf1N1B4_3849 0.70 -1.6 4,270,922 + Pf1N1B4_3849 0.70 +0.5 4,270,952 + Pf1N1B4_3849 0.74 +0.7 4,270,952 + Pf1N1B4_3849 0.74 +1.8 4,270,953 - Pf1N1B4_3849 0.74 +0.5 4,270,953 - Pf1N1B4_3849 0.74 +0.6 4,271,360 + -1.2 4,271,360 + +0.8 4,271,360 + +1.3 4,271,361 - +1.2 4,271,361 - -1.6 4,271,361 - -0.5 4,271,361 - -2.5 4,271,401 + -0.5 4,271,401 + +1.8 4,271,402 - -0.6 4,271,402 - -1.2 4,271,402 - +0.4 4,271,402 - +1.1 4,271,444 + +2.6 4,271,469 - Pf1N1B4_3850 0.11 -2.8 4,271,533 + Pf1N1B4_3850 0.16 -1.2 4,271,533 + Pf1N1B4_3850 0.16 -2.2 4,271,534 - Pf1N1B4_3850 0.16 -1.8 4,271,564 + Pf1N1B4_3850 0.18 +1.0 4,271,600 - Pf1N1B4_3850 0.21 +0.0 4,271,600 - Pf1N1B4_3850 0.21 +0.1 4,271,600 - Pf1N1B4_3850 0.21 +1.1 4,271,615 + Pf1N1B4_3850 0.22 -0.9 4,271,616 - Pf1N1B4_3850 0.22 -0.2 4,271,616 - -1.8 4,271,853 - Pf1N1B4_3850 0.40 -0.5 4,271,861 + Pf1N1B4_3850 0.41 +0.2 4,271,862 - Pf1N1B4_3850 0.41 -1.8 4,271,862 - -2.2 4,271,948 + Pf1N1B4_3850 0.47 +0.8 4,271,949 - Pf1N1B4_3850 0.48 -0.9 4,271,949 - Pf1N1B4_3850 0.48 -0.2 4,271,949 - Pf1N1B4_3850 0.48 -1.2 4,271,949 - Pf1N1B4_3850 0.48 -0.8 4,271,949 - Pf1N1B4_3850 0.48 +0.6 4,272,089 + Pf1N1B4_3850 0.58 -2.5 4,272,090 - Pf1N1B4_3850 0.58 +1.0 4,272,090 - Pf1N1B4_3850 0.58 +0.4 4,272,090 - Pf1N1B4_3850 0.58 -1.2 4,272,117 - -0.2 4,272,117 - -1.8 4,272,332 + Pf1N1B4_3850 0.77 -1.5 4,272,332 + Pf1N1B4_3850 0.77 +0.8 4,272,332 + Pf1N1B4_3850 0.77 +2.4 4,272,332 + Pf1N1B4_3850 0.77 +1.8 4,272,332 + Pf1N1B4_3850 0.77 +0.4 4,272,333 - Pf1N1B4_3850 0.77 -0.2 4,272,333 - Pf1N1B4_3850 0.77 -0.2 4,272,333 - Pf1N1B4_3850 0.77 -1.6 4,272,333 - Pf1N1B4_3850 0.77 -0.2 4,272,333 - Pf1N1B4_3850 0.77 -1.5 4,272,333 - -1.2 4,272,333 - Pf1N1B4_3850 0.77 +1.4 4,272,352 + Pf1N1B4_3850 0.78 -1.1 4,272,352 + Pf1N1B4_3850 0.78 +1.1 4,272,352 + Pf1N1B4_3850 0.78 -3.2 4,272,352 + Pf1N1B4_3850 0.78 -1.8 4,272,366 - Pf1N1B4_3850 0.79 -0.8 4,272,551 + +0.8 4,272,647 + +0.2 4,272,647 + -0.2 4,272,648 - -0.6 4,272,766 + Pf1N1B4_3851 0.11 -1.2 4,272,766 + Pf1N1B4_3851 0.11 +0.4 4,272,766 + -0.8 4,272,766 + Pf1N1B4_3851 0.11 -0.4 4,272,766 + Pf1N1B4_3851 0.11 +0.8 4,272,766 + Pf1N1B4_3851 0.11 -0.2 4,272,766 + Pf1N1B4_3851 0.11 +1.4 4,272,766 + Pf1N1B4_3851 0.11 -1.8 4,272,767 - Pf1N1B4_3851 0.11 -0.2 4,272,767 - Pf1N1B4_3851 0.11 +0.2 4,273,021 + Pf1N1B4_3851 0.40 -0.6 4,273,021 + Pf1N1B4_3851 0.40 -0.2 4,273,022 - Pf1N1B4_3851 0.41 -1.2 4,273,354 + Pf1N1B4_3851 0.79 -0.2 4,273,354 + Pf1N1B4_3851 0.79 -0.2 4,273,354 + Pf1N1B4_3851 0.79 +0.8 4,273,355 - Pf1N1B4_3851 0.80 +0.8 4,273,355 - Pf1N1B4_3851 0.80 -0.7 4,273,516 + +1.5 4,273,516 + -1.2 4,273,516 + -1.4 4,273,516 + +0.4 4,273,516 + +0.7 4,273,516 + +0.8 4,273,517 - -0.9 4,273,517 - +1.0 4,273,517 - +1.0 4,273,517 - -0.2 4,273,517 - -0.4 4,273,517 - +0.4 4,273,517 - +0.5 4,273,517 - +0.9 4,273,517 - +0.4
Or see this region's nucleotide sequence