Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_351

Experiment: Fusidic 0.0625 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_350 and Pf1N1B4_351 overlap by 1 nucleotidesPf1N1B4_351 and Pf1N1B4_352 are separated by 1 nucleotidesPf1N1B4_352 and Pf1N1B4_353 are separated by 223 nucleotides Pf1N1B4_350 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 415,241 to 416,335 _350 Pf1N1B4_351 - Phosphoserine aminotransferase (EC 2.6.1.52), at 416,335 to 417,213 _351 Pf1N1B4_352 - Phosphoserine aminotransferase (EC 2.6.1.52), at 417,215 to 417,427 _352 Pf1N1B4_353 - DNA gyrase subunit A (EC 5.99.1.3), at 417,651 to 420,320 _353 Position (kb) 416 417 418Strain fitness (log2 ratio) -2 -1 0 1 2at 415.365 kb on + strand, within Pf1N1B4_350at 415.365 kb on + strand, within Pf1N1B4_350at 415.365 kb on + strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.366 kb on - strand, within Pf1N1B4_350at 415.376 kb on - strand, within Pf1N1B4_350at 415.376 kb on - strand, within Pf1N1B4_350at 415.376 kb on - strand, within Pf1N1B4_350at 415.755 kb on + strand, within Pf1N1B4_350at 415.756 kb on - strand, within Pf1N1B4_350at 415.756 kb on - strandat 415.756 kb on - strand, within Pf1N1B4_350at 415.756 kb on - strand, within Pf1N1B4_350at 415.756 kb on - strand, within Pf1N1B4_350at 415.756 kb on - strand, within Pf1N1B4_350at 415.756 kb on - strand, within Pf1N1B4_350at 416.043 kb on + strand, within Pf1N1B4_350at 416.226 kb on - strandat 416.226 kb on - strandat 417.452 kb on + strandat 417.453 kb on - strandat 417.453 kb on - strandat 417.453 kb on - strandat 417.453 kb on - strandat 417.653 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml
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415,365 + Pf1N1B4_350 0.11 +0.9
415,365 + Pf1N1B4_350 0.11 -1.8
415,365 + Pf1N1B4_350 0.11 -0.1
415,366 - Pf1N1B4_350 0.11 -2.2
415,366 - Pf1N1B4_350 0.11 -1.1
415,366 - Pf1N1B4_350 0.11 +0.7
415,366 - Pf1N1B4_350 0.11 -0.2
415,366 - Pf1N1B4_350 0.11 -0.5
415,366 - Pf1N1B4_350 0.11 +1.5
415,366 - Pf1N1B4_350 0.11 +1.5
415,366 - Pf1N1B4_350 0.11 -0.8
415,366 - Pf1N1B4_350 0.11 -0.1
415,366 - Pf1N1B4_350 0.11 +1.3
415,366 - Pf1N1B4_350 0.11 -0.8
415,366 - Pf1N1B4_350 0.11 +0.5
415,366 - Pf1N1B4_350 0.11 +0.9
415,366 - Pf1N1B4_350 0.11 -0.1
415,366 - Pf1N1B4_350 0.11 +0.3
415,366 - Pf1N1B4_350 0.11 +1.9
415,366 - Pf1N1B4_350 0.11 +1.1
415,366 - Pf1N1B4_350 0.11 -0.1
415,366 - Pf1N1B4_350 0.11 +1.3
415,376 - Pf1N1B4_350 0.12 +0.8
415,376 - Pf1N1B4_350 0.12 +1.2
415,376 - Pf1N1B4_350 0.12 +0.4
415,755 + Pf1N1B4_350 0.47 -0.5
415,756 - Pf1N1B4_350 0.47 -1.6
415,756 - +2.5
415,756 - Pf1N1B4_350 0.47 -0.1
415,756 - Pf1N1B4_350 0.47 -0.1
415,756 - Pf1N1B4_350 0.47 +0.4
415,756 - Pf1N1B4_350 0.47 +1.5
415,756 - Pf1N1B4_350 0.47 -0.1
416,043 + Pf1N1B4_350 0.73 +0.2
416,226 - -0.1
416,226 - -1.6
417,452 + +0.5
417,453 - -1.1
417,453 - +0.4
417,453 - -2.1
417,453 - -1.1
417,653 - +1.1

Or see this region's nucleotide sequence