Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3168 and Pf1N1B4_3169 are separated by 44 nucleotides Pf1N1B4_3169 and Pf1N1B4_3170 are separated by 167 nucleotides Pf1N1B4_3170 and Pf1N1B4_3171 are separated by 177 nucleotides Pf1N1B4_3171 and Pf1N1B4_3172 are separated by 354 nucleotides
Pf1N1B4_3168 - AmpG permease, at 3,550,979 to 3,552,538
_3168
Pf1N1B4_3169 - methylated-DNA--protein-cysteine methyltransferase-related protein, at 3,552,583 to 3,552,936
_3169
Pf1N1B4_3170 - Peptide chain release factor RF-3, at 3,553,104 to 3,554,111
_3170
Pf1N1B4_3171 - Cold shock protein CspA, at 3,554,289 to 3,554,498
_3171
Pf1N1B4_3172 - Deoxycytidine triphosphate deaminase (EC 3.5.4.13), at 3,554,853 to 3,555,419
_3172
Position (kb)
3553
3554
3555 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3552.445 kb on - strand at 3552.624 kb on + strand, within Pf1N1B4_3169 at 3552.836 kb on + strand, within Pf1N1B4_3169 at 3552.837 kb on - strand, within Pf1N1B4_3169 at 3552.878 kb on + strand, within Pf1N1B4_3169 at 3552.878 kb on + strand, within Pf1N1B4_3169 at 3552.878 kb on + strand, within Pf1N1B4_3169 at 3552.879 kb on - strand, within Pf1N1B4_3169 at 3552.879 kb on - strand, within Pf1N1B4_3169 at 3552.879 kb on - strand, within Pf1N1B4_3169 at 3552.976 kb on + strand at 3552.981 kb on + strand at 3552.982 kb on - strand at 3552.982 kb on - strand at 3553.041 kb on + strand at 3553.042 kb on - strand at 3553.042 kb on - strand at 3553.103 kb on + strand at 3553.104 kb on - strand at 3553.104 kb on - strand at 3553.104 kb on - strand at 3553.213 kb on + strand, within Pf1N1B4_3170 at 3553.214 kb on - strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.468 kb on + strand, within Pf1N1B4_3170 at 3553.468 kb on + strand, within Pf1N1B4_3170 at 3553.469 kb on - strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.531 kb on - strand, within Pf1N1B4_3170 at 3553.531 kb on - strand, within Pf1N1B4_3170 at 3553.531 kb on - strand, within Pf1N1B4_3170 at 3553.593 kb on + strand, within Pf1N1B4_3170 at 3553.593 kb on + strand, within Pf1N1B4_3170 at 3553.593 kb on + strand, within Pf1N1B4_3170 at 3553.593 kb on + strand, within Pf1N1B4_3170 at 3553.593 kb on + strand, within Pf1N1B4_3170 at 3553.594 kb on - strand, within Pf1N1B4_3170 at 3553.594 kb on - strand, within Pf1N1B4_3170 at 3553.594 kb on - strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.619 kb on - strand, within Pf1N1B4_3170 at 3553.619 kb on - strand, within Pf1N1B4_3170 at 3553.619 kb on - strand at 3553.619 kb on - strand, within Pf1N1B4_3170 at 3553.619 kb on - strand, within Pf1N1B4_3170 at 3553.619 kb on - strand, within Pf1N1B4_3170 at 3553.701 kb on + strand, within Pf1N1B4_3170 at 3553.701 kb on + strand, within Pf1N1B4_3170 at 3553.824 kb on + strand, within Pf1N1B4_3170 at 3553.824 kb on + strand, within Pf1N1B4_3170 at 3553.824 kb on + strand, within Pf1N1B4_3170 at 3553.825 kb on - strand, within Pf1N1B4_3170 at 3553.857 kb on + strand, within Pf1N1B4_3170 at 3553.857 kb on + strand, within Pf1N1B4_3170 at 3553.857 kb on + strand, within Pf1N1B4_3170 at 3553.857 kb on + strand, within Pf1N1B4_3170 at 3553.881 kb on + strand, within Pf1N1B4_3170 at 3553.881 kb on + strand, within Pf1N1B4_3170 at 3553.882 kb on - strand, within Pf1N1B4_3170 at 3553.882 kb on - strand, within Pf1N1B4_3170 at 3553.882 kb on - strand at 3553.882 kb on - strand, within Pf1N1B4_3170 at 3553.954 kb on + strand, within Pf1N1B4_3170 at 3554.043 kb on - strand at 3554.158 kb on + strand at 3554.169 kb on + strand at 3554.169 kb on + strand at 3554.169 kb on + strand at 3554.169 kb on + strand at 3554.169 kb on + strand at 3554.169 kb on + strand at 3554.170 kb on - strand at 3554.170 kb on - strand at 3554.170 kb on - strand at 3554.170 kb on - strand at 3554.179 kb on + strand at 3554.179 kb on + strand at 3554.179 kb on + strand at 3554.180 kb on - strand at 3554.180 kb on - strand at 3554.272 kb on - strand at 3554.275 kb on - strand at 3554.284 kb on - strand at 3554.284 kb on - strand at 3554.291 kb on - strand at 3554.291 kb on - strand at 3554.291 kb on - strand at 3554.295 kb on - strand at 3554.504 kb on - strand at 3554.504 kb on - strand at 3554.504 kb on - strand at 3554.504 kb on - strand at 3554.504 kb on - strand at 3554.504 kb on - strand at 3554.522 kb on - strand at 3554.522 kb on - strand at 3554.522 kb on - strand at 3554.583 kb on - strand at 3554.583 kb on - strand at 3554.607 kb on + strand at 3554.608 kb on - strand at 3554.608 kb on - strand at 3554.608 kb on - strand at 3554.615 kb on - strand at 3554.656 kb on - strand at 3555.020 kb on + strand, within Pf1N1B4_3172
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 3,552,445 - +0.5 3,552,624 + Pf1N1B4_3169 0.12 -0.3 3,552,836 + Pf1N1B4_3169 0.71 -1.4 3,552,837 - Pf1N1B4_3169 0.72 -1.0 3,552,878 + Pf1N1B4_3169 0.83 +0.2 3,552,878 + Pf1N1B4_3169 0.83 +1.2 3,552,878 + Pf1N1B4_3169 0.83 +1.2 3,552,879 - Pf1N1B4_3169 0.84 +0.4 3,552,879 - Pf1N1B4_3169 0.84 -1.3 3,552,879 - Pf1N1B4_3169 0.84 -2.1 3,552,976 + -2.4 3,552,981 + -1.4 3,552,982 - +0.2 3,552,982 - +1.0 3,553,041 + +0.5 3,553,042 - +0.2 3,553,042 - -2.6 3,553,103 + -0.1 3,553,104 - -0.8 3,553,104 - -3.0 3,553,104 - -1.8 3,553,213 + Pf1N1B4_3170 0.11 -1.1 3,553,214 - Pf1N1B4_3170 0.11 -0.4 3,553,329 + Pf1N1B4_3170 0.22 -1.4 3,553,329 + Pf1N1B4_3170 0.22 -0.8 3,553,329 + Pf1N1B4_3170 0.22 -0.2 3,553,329 + Pf1N1B4_3170 0.22 +0.1 3,553,329 + Pf1N1B4_3170 0.22 -0.8 3,553,329 + Pf1N1B4_3170 0.22 -0.8 3,553,330 - Pf1N1B4_3170 0.22 -1.8 3,553,330 - Pf1N1B4_3170 0.22 -1.8 3,553,330 - Pf1N1B4_3170 0.22 +2.4 3,553,330 - Pf1N1B4_3170 0.22 -1.5 3,553,330 - Pf1N1B4_3170 0.22 -0.9 3,553,330 - Pf1N1B4_3170 0.22 -0.8 3,553,330 - Pf1N1B4_3170 0.22 -0.4 3,553,468 + Pf1N1B4_3170 0.36 -0.5 3,553,468 + Pf1N1B4_3170 0.36 +0.2 3,553,469 - Pf1N1B4_3170 0.36 -1.2 3,553,530 + Pf1N1B4_3170 0.42 -0.8 3,553,530 + Pf1N1B4_3170 0.42 +0.2 3,553,530 + Pf1N1B4_3170 0.42 -1.8 3,553,530 + Pf1N1B4_3170 0.42 -1.5 3,553,530 + Pf1N1B4_3170 0.42 +1.8 3,553,531 - Pf1N1B4_3170 0.42 -0.4 3,553,531 - Pf1N1B4_3170 0.42 +2.0 3,553,531 - Pf1N1B4_3170 0.42 +0.4 3,553,593 + Pf1N1B4_3170 0.49 -0.8 3,553,593 + Pf1N1B4_3170 0.49 -1.4 3,553,593 + Pf1N1B4_3170 0.49 -1.3 3,553,593 + Pf1N1B4_3170 0.49 +0.6 3,553,593 + Pf1N1B4_3170 0.49 -1.8 3,553,594 - Pf1N1B4_3170 0.49 +3.7 3,553,594 - Pf1N1B4_3170 0.49 -2.8 3,553,594 - Pf1N1B4_3170 0.49 -1.4 3,553,618 + Pf1N1B4_3170 0.51 -0.2 3,553,618 + Pf1N1B4_3170 0.51 +0.2 3,553,618 + Pf1N1B4_3170 0.51 -0.4 3,553,618 + Pf1N1B4_3170 0.51 +0.2 3,553,618 + Pf1N1B4_3170 0.51 -1.8 3,553,618 + Pf1N1B4_3170 0.51 -1.4 3,553,619 - Pf1N1B4_3170 0.51 -1.4 3,553,619 - Pf1N1B4_3170 0.51 -0.4 3,553,619 - -0.8 3,553,619 - Pf1N1B4_3170 0.51 -2.4 3,553,619 - Pf1N1B4_3170 0.51 -2.4 3,553,619 - Pf1N1B4_3170 0.51 -0.8 3,553,701 + Pf1N1B4_3170 0.59 -1.4 3,553,701 + Pf1N1B4_3170 0.59 -1.0 3,553,824 + Pf1N1B4_3170 0.71 -0.8 3,553,824 + Pf1N1B4_3170 0.71 -1.4 3,553,824 + Pf1N1B4_3170 0.71 -1.8 3,553,825 - Pf1N1B4_3170 0.72 -1.4 3,553,857 + Pf1N1B4_3170 0.75 -1.1 3,553,857 + Pf1N1B4_3170 0.75 -1.4 3,553,857 + Pf1N1B4_3170 0.75 -0.8 3,553,857 + Pf1N1B4_3170 0.75 -2.0 3,553,881 + Pf1N1B4_3170 0.77 +0.2 3,553,881 + Pf1N1B4_3170 0.77 -0.8 3,553,882 - Pf1N1B4_3170 0.77 -1.4 3,553,882 - Pf1N1B4_3170 0.77 -2.1 3,553,882 - -0.8 3,553,882 - Pf1N1B4_3170 0.77 +1.2 3,553,954 + Pf1N1B4_3170 0.84 -1.4 3,554,043 - -1.8 3,554,158 + +0.4 3,554,169 + -0.8 3,554,169 + +0.2 3,554,169 + +0.6 3,554,169 + +1.2 3,554,169 + -0.5 3,554,169 + +0.1 3,554,170 - +0.2 3,554,170 - -1.1 3,554,170 - -1.4 3,554,170 - +0.2 3,554,179 + -0.8 3,554,179 + -2.1 3,554,179 + +0.9 3,554,180 - -0.8 3,554,180 - +1.2 3,554,272 - -2.6 3,554,275 - +1.1 3,554,284 - -1.4 3,554,284 - -1.8 3,554,291 - -0.8 3,554,291 - -0.2 3,554,291 - +2.5 3,554,295 - -1.4 3,554,504 - +0.2 3,554,504 - -0.8 3,554,504 - -0.8 3,554,504 - -1.2 3,554,504 - -0.1 3,554,504 - -0.1 3,554,522 - -1.5 3,554,522 - -2.6 3,554,522 - +1.2 3,554,583 - -1.8 3,554,583 - +0.6 3,554,607 + +0.8 3,554,608 - -0.8 3,554,608 - -1.8 3,554,608 - -1.8 3,554,615 - -0.1 3,554,656 - -2.4 3,555,020 + Pf1N1B4_3172 0.29 -0.4
Or see this region's nucleotide sequence