Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3145 and Pf1N1B4_3146 are separated by 488 nucleotides Pf1N1B4_3146 and Pf1N1B4_3147 are separated by 0 nucleotides Pf1N1B4_3147 and Pf1N1B4_3148 are separated by 189 nucleotides
Pf1N1B4_3145 - Decarboxylase family protein, at 3,527,322 to 3,528,440
_3145
Pf1N1B4_3146 - FIG00957354: hypothetical protein, at 3,528,929 to 3,529,123
_3146
Pf1N1B4_3147 - Putative NADPH-quinone reductase (modulator of drug activity B), at 3,529,124 to 3,529,549
_3147
Pf1N1B4_3148 - FIG074102: hypothetical protein, at 3,529,739 to 3,530,482
_3148
Position (kb)
3529
3530 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3528.136 kb on + strand, within Pf1N1B4_3145 at 3528.136 kb on + strand, within Pf1N1B4_3145 at 3528.137 kb on - strand, within Pf1N1B4_3145 at 3528.137 kb on - strand, within Pf1N1B4_3145 at 3528.150 kb on + strand, within Pf1N1B4_3145 at 3528.586 kb on + strand at 3528.587 kb on - strand at 3528.617 kb on + strand at 3528.618 kb on - strand at 3528.618 kb on - strand at 3528.679 kb on + strand at 3528.679 kb on + strand at 3528.743 kb on + strand at 3528.744 kb on - strand at 3528.744 kb on - strand at 3528.791 kb on + strand at 3528.791 kb on + strand at 3528.791 kb on + strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.810 kb on + strand at 3528.813 kb on + strand at 3528.813 kb on + strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.818 kb on + strand at 3528.819 kb on - strand at 3528.819 kb on - strand at 3528.965 kb on + strand, within Pf1N1B4_3146 at 3529.058 kb on + strand, within Pf1N1B4_3146 at 3529.126 kb on - strand at 3529.293 kb on + strand, within Pf1N1B4_3147 at 3529.293 kb on + strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.436 kb on + strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.538 kb on - strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.619 kb on - strand at 3529.620 kb on + strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.681 kb on + strand at 3529.681 kb on + strand at 3529.869 kb on + strand, within Pf1N1B4_3148 at 3529.870 kb on - strand, within Pf1N1B4_3148 at 3529.927 kb on + strand, within Pf1N1B4_3148 at 3530.063 kb on + strand, within Pf1N1B4_3148 at 3530.064 kb on - strand, within Pf1N1B4_3148 at 3530.064 kb on - strand, within Pf1N1B4_3148 at 3530.084 kb on + strand at 3530.105 kb on - strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.361 kb on - strand, within Pf1N1B4_3148 at 3530.384 kb on + strand, within Pf1N1B4_3148 at 3530.385 kb on - strand, within Pf1N1B4_3148 at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.493 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 3,528,136 + Pf1N1B4_3145 0.73 -0.5 3,528,136 + Pf1N1B4_3145 0.73 +2.2 3,528,137 - Pf1N1B4_3145 0.73 -0.5 3,528,137 - Pf1N1B4_3145 0.73 -0.8 3,528,150 + Pf1N1B4_3145 0.74 -1.1 3,528,586 + -0.4 3,528,587 - -0.8 3,528,617 + +0.9 3,528,618 - +1.2 3,528,618 - +0.9 3,528,679 + -2.1 3,528,679 + -0.4 3,528,743 + +0.5 3,528,744 - +0.2 3,528,744 - -2.6 3,528,791 + +2.5 3,528,791 + -1.8 3,528,791 + -0.4 3,528,792 - -0.2 3,528,792 - +0.8 3,528,792 - -0.1 3,528,792 - -0.8 3,528,810 + +0.2 3,528,813 + +1.2 3,528,813 + -2.1 3,528,814 - +0.2 3,528,814 - +0.2 3,528,814 - +1.2 3,528,814 - +1.2 3,528,814 - +0.5 3,528,818 + -0.2 3,528,819 - -2.4 3,528,819 - -0.4 3,528,965 + Pf1N1B4_3146 0.18 -0.4 3,529,058 + Pf1N1B4_3146 0.66 +2.5 3,529,126 - -0.3 3,529,293 + Pf1N1B4_3147 0.40 -1.2 3,529,293 + Pf1N1B4_3147 0.40 +0.2 3,529,294 - Pf1N1B4_3147 0.40 +0.8 3,529,294 - Pf1N1B4_3147 0.40 -0.9 3,529,294 - Pf1N1B4_3147 0.40 +0.2 3,529,378 + Pf1N1B4_3147 0.60 -2.1 3,529,378 + Pf1N1B4_3147 0.60 -2.6 3,529,378 + Pf1N1B4_3147 0.60 +0.2 3,529,378 + Pf1N1B4_3147 0.60 -2.4 3,529,378 + Pf1N1B4_3147 0.60 +0.9 3,529,378 + Pf1N1B4_3147 0.60 +0.2 3,529,378 + Pf1N1B4_3147 0.60 +0.2 3,529,379 - Pf1N1B4_3147 0.60 -0.9 3,529,379 - Pf1N1B4_3147 0.60 -2.6 3,529,379 - Pf1N1B4_3147 0.60 +0.8 3,529,379 - Pf1N1B4_3147 0.60 -1.1 3,529,379 - Pf1N1B4_3147 0.60 -0.5 3,529,379 - Pf1N1B4_3147 0.60 +0.8 3,529,379 - Pf1N1B4_3147 0.60 +1.8 3,529,379 - Pf1N1B4_3147 0.60 -1.5 3,529,379 - Pf1N1B4_3147 0.60 -1.1 3,529,380 + Pf1N1B4_3147 0.60 -1.5 3,529,380 + Pf1N1B4_3147 0.60 -3.0 3,529,380 + Pf1N1B4_3147 0.60 -0.4 3,529,381 - Pf1N1B4_3147 0.60 -1.0 3,529,381 - Pf1N1B4_3147 0.60 +0.8 3,529,381 - Pf1N1B4_3147 0.60 -1.1 3,529,381 - Pf1N1B4_3147 0.60 -0.8 3,529,436 + Pf1N1B4_3147 0.73 -0.8 3,529,437 - Pf1N1B4_3147 0.73 -1.4 3,529,437 - Pf1N1B4_3147 0.73 -0.5 3,529,437 - Pf1N1B4_3147 0.73 -0.4 3,529,437 - Pf1N1B4_3147 0.73 -0.5 3,529,437 - Pf1N1B4_3147 0.73 -2.4 3,529,538 - -1.1 3,529,618 + -1.2 3,529,618 + +0.2 3,529,618 + -0.8 3,529,618 + +2.8 3,529,618 + +0.2 3,529,618 + +0.9 3,529,619 - -2.6 3,529,620 + -0.2 3,529,621 - +1.4 3,529,621 - +1.3 3,529,621 - +0.2 3,529,621 - -0.4 3,529,681 + +0.0 3,529,681 + -1.1 3,529,869 + Pf1N1B4_3148 0.17 +1.5 3,529,870 - Pf1N1B4_3148 0.18 -0.2 3,529,927 + Pf1N1B4_3148 0.25 -0.8 3,530,063 + Pf1N1B4_3148 0.44 +1.0 3,530,064 - Pf1N1B4_3148 0.44 +0.2 3,530,064 - Pf1N1B4_3148 0.44 -1.5 3,530,084 + -0.8 3,530,105 - Pf1N1B4_3148 0.49 +0.2 3,530,360 + Pf1N1B4_3148 0.83 +0.2 3,530,360 + Pf1N1B4_3148 0.83 +0.5 3,530,360 + Pf1N1B4_3148 0.83 -1.1 3,530,361 - Pf1N1B4_3148 0.84 +0.2 3,530,384 + Pf1N1B4_3148 0.87 +1.2 3,530,385 - Pf1N1B4_3148 0.87 +1.5 3,530,454 + +0.2 3,530,454 + +0.2 3,530,454 + +0.2 3,530,454 + +0.2 3,530,454 + +2.2 3,530,493 + +0.9
Or see this region's nucleotide sequence