Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_301 and Pf1N1B4_302 are separated by 259 nucleotides Pf1N1B4_302 and Pf1N1B4_303 are separated by 105 nucleotides Pf1N1B4_303 and Pf1N1B4_304 are separated by 234 nucleotides
Pf1N1B4_301 - FIG00957636: hypothetical protein, at 354,986 to 355,294
_301
Pf1N1B4_302 - Membrane protein, distant similarity to thiosulphate:quinone oxidoreductase DoxD, at 355,554 to 355,988
_302
Pf1N1B4_303 - Transglycosylase, Slt family, at 356,094 to 357,515
_303
Pf1N1B4_304 - Deoxyribonuclease TatD, at 357,750 to 358,559
_304
Position (kb) 356
357
358 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 355.185 kb on + strand, within Pf1N1B4_301 at 355.186 kb on - strand, within Pf1N1B4_301 at 355.271 kb on + strand at 355.271 kb on + strand at 355.271 kb on + strand at 355.272 kb on - strand at 355.272 kb on - strand at 355.272 kb on - strand at 355.283 kb on + strand at 355.283 kb on + strand at 355.283 kb on + strand at 355.283 kb on + strand at 355.284 kb on - strand at 355.364 kb on + strand at 355.390 kb on - strand at 355.426 kb on + strand at 355.426 kb on + strand at 355.427 kb on - strand at 355.458 kb on + strand at 355.458 kb on + strand at 355.458 kb on + strand at 355.646 kb on - strand, within Pf1N1B4_302 at 355.655 kb on + strand, within Pf1N1B4_302 at 355.694 kb on + strand, within Pf1N1B4_302 at 355.695 kb on - strand, within Pf1N1B4_302 at 355.837 kb on + strand, within Pf1N1B4_302 at 355.837 kb on + strand, within Pf1N1B4_302 at 355.837 kb on + strand, within Pf1N1B4_302 at 355.837 kb on + strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.926 kb on - strand, within Pf1N1B4_302 at 355.965 kb on + strand at 355.965 kb on + strand at 355.965 kb on + strand at 355.965 kb on + strand at 355.965 kb on + strand at 355.966 kb on - strand at 355.966 kb on - strand at 355.966 kb on - strand at 355.966 kb on - strand at 355.966 kb on - strand at 355.966 kb on - strand at 356.161 kb on + strand at 356.161 kb on + strand at 356.161 kb on + strand at 356.161 kb on + strand at 356.161 kb on + strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.164 kb on + strand at 356.164 kb on + strand at 356.164 kb on + strand at 356.164 kb on + strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.177 kb on + strand at 356.177 kb on + strand at 356.180 kb on + strand at 356.180 kb on + strand at 356.180 kb on + strand at 356.180 kb on + strand at 356.181 kb on - strand at 356.181 kb on - strand at 356.181 kb on - strand at 356.181 kb on - strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.314 kb on + strand, within Pf1N1B4_303 at 356.314 kb on + strand, within Pf1N1B4_303 at 356.314 kb on + strand, within Pf1N1B4_303 at 356.314 kb on + strand, within Pf1N1B4_303 at 356.315 kb on - strand, within Pf1N1B4_303 at 356.395 kb on + strand, within Pf1N1B4_303 at 356.395 kb on + strand, within Pf1N1B4_303 at 356.396 kb on - strand, within Pf1N1B4_303 at 356.435 kb on + strand, within Pf1N1B4_303 at 356.438 kb on + strand, within Pf1N1B4_303 at 356.438 kb on + strand, within Pf1N1B4_303 at 356.439 kb on - strand, within Pf1N1B4_303 at 356.439 kb on - strand, within Pf1N1B4_303 at 356.439 kb on - strand, within Pf1N1B4_303 at 356.595 kb on + strand, within Pf1N1B4_303 at 356.657 kb on - strand, within Pf1N1B4_303 at 356.711 kb on - strand, within Pf1N1B4_303 at 356.711 kb on - strand, within Pf1N1B4_303 at 356.711 kb on - strand, within Pf1N1B4_303 at 356.711 kb on - strand, within Pf1N1B4_303 at 356.711 kb on - strand, within Pf1N1B4_303 at 356.773 kb on + strand, within Pf1N1B4_303 at 356.773 kb on + strand, within Pf1N1B4_303 at 357.061 kb on + strand, within Pf1N1B4_303 at 357.125 kb on + strand at 357.156 kb on + strand, within Pf1N1B4_303 at 357.156 kb on + strand, within Pf1N1B4_303 at 357.156 kb on + strand, within Pf1N1B4_303 at 357.156 kb on + strand, within Pf1N1B4_303 at 357.156 kb on + strand, within Pf1N1B4_303 at 357.156 kb on + strand, within Pf1N1B4_303 at 357.157 kb on - strand, within Pf1N1B4_303 at 357.157 kb on - strand, within Pf1N1B4_303 at 357.157 kb on - strand, within Pf1N1B4_303 at 357.157 kb on - strand, within Pf1N1B4_303 at 357.157 kb on - strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.278 kb on - strand at 357.304 kb on + strand, within Pf1N1B4_303 at 357.598 kb on + strand at 357.671 kb on + strand at 357.681 kb on + strand at 357.681 kb on + strand at 357.681 kb on + strand at 357.698 kb on + strand at 357.700 kb on + strand at 357.700 kb on + strand at 357.706 kb on + strand at 357.717 kb on + strand at 357.718 kb on - strand at 357.743 kb on + strand at 357.744 kb on - strand at 357.744 kb on - strand at 357.744 kb on - strand at 357.828 kb on + strand at 357.829 kb on - strand at 358.347 kb on - strand, within Pf1N1B4_304 at 358.384 kb on - strand, within Pf1N1B4_304 at 358.384 kb on - strand, within Pf1N1B4_304 at 358.441 kb on - strand, within Pf1N1B4_304
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 355,185 + Pf1N1B4_301 0.64 -0.1 355,186 - Pf1N1B4_301 0.65 +0.3 355,271 + -0.1 355,271 + -0.1 355,271 + +2.3 355,272 - +1.5 355,272 - +3.5 355,272 - -1.1 355,283 + +0.7 355,283 + +1.2 355,283 + -0.1 355,283 + +2.2 355,284 - +1.2 355,364 + +1.0 355,390 - -0.1 355,426 + +3.2 355,426 + +0.5 355,427 - -2.1 355,458 + +0.3 355,458 + +0.7 355,458 + +0.6 355,646 - Pf1N1B4_302 0.21 +0.4 355,655 + Pf1N1B4_302 0.23 -0.1 355,694 + Pf1N1B4_302 0.32 +1.1 355,695 - Pf1N1B4_302 0.32 +1.1 355,837 + Pf1N1B4_302 0.65 +0.0 355,837 + Pf1N1B4_302 0.65 +0.2 355,837 + Pf1N1B4_302 0.65 +1.9 355,837 + Pf1N1B4_302 0.65 -0.8 355,838 - Pf1N1B4_302 0.65 +0.6 355,838 - Pf1N1B4_302 0.65 +1.5 355,838 - Pf1N1B4_302 0.65 +0.9 355,838 - Pf1N1B4_302 0.65 -1.7 355,838 - Pf1N1B4_302 0.65 +1.5 355,838 - Pf1N1B4_302 0.65 +0.9 355,838 - Pf1N1B4_302 0.65 -0.1 355,926 - Pf1N1B4_302 0.86 +0.2 355,965 + +1.5 355,965 + +1.7 355,965 + -2.9 355,965 + +0.4 355,965 + +0.7 355,966 - +0.1 355,966 - -0.1 355,966 - -1.5 355,966 - +1.5 355,966 - +2.0 355,966 - +0.2 356,161 + +1.2 356,161 + -0.1 356,161 + -1.4 356,161 + +0.5 356,161 + +0.3 356,162 - +1.1 356,162 - +1.2 356,162 - +1.9 356,162 - +0.7 356,162 - -1.7 356,162 - +0.5 356,162 - +0.1 356,162 - -0.1 356,164 + -0.4 356,164 + +1.1 356,164 + +0.6 356,164 + -0.1 356,165 - -0.1 356,165 - +1.5 356,165 - +0.9 356,165 - -1.1 356,165 - -0.1 356,165 - +1.5 356,165 - +0.2 356,165 - -1.3 356,165 - +0.5 356,165 - +0.0 356,165 - +2.7 356,165 - -0.6 356,165 - -0.5 356,165 - -2.4 356,165 - +0.7 356,165 - +0.9 356,165 - -0.4 356,177 + -2.9 356,177 + +1.7 356,180 + +0.9 356,180 + +1.4 356,180 + -0.1 356,180 + -1.7 356,181 - +2.7 356,181 - +0.1 356,181 - -0.5 356,181 - +0.9 356,185 + +0.5 356,185 + +0.9 356,185 + -1.2 356,185 + -0.5 356,185 + +0.7 356,185 + -0.3 356,185 + +0.3 356,185 + -0.5 356,186 - -0.5 356,186 - +0.3 356,186 - +1.7 356,186 - -0.8 356,186 - -1.1 356,186 - +0.4 356,186 - -0.7 356,186 - -0.2 356,314 + Pf1N1B4_303 0.15 +1.9 356,314 + Pf1N1B4_303 0.15 -0.7 356,314 + Pf1N1B4_303 0.15 +0.9 356,314 + Pf1N1B4_303 0.15 +0.7 356,315 - Pf1N1B4_303 0.16 +1.3 356,395 + Pf1N1B4_303 0.21 -0.1 356,395 + Pf1N1B4_303 0.21 -0.9 356,396 - Pf1N1B4_303 0.21 -0.7 356,435 + Pf1N1B4_303 0.24 -0.7 356,438 + Pf1N1B4_303 0.24 +0.3 356,438 + Pf1N1B4_303 0.24 +0.5 356,439 - Pf1N1B4_303 0.24 +1.2 356,439 - Pf1N1B4_303 0.24 +0.5 356,439 - Pf1N1B4_303 0.24 -0.1 356,595 + Pf1N1B4_303 0.35 +0.4 356,657 - Pf1N1B4_303 0.40 +1.7 356,711 - Pf1N1B4_303 0.43 +0.7 356,711 - Pf1N1B4_303 0.43 +0.6 356,711 - Pf1N1B4_303 0.43 +0.3 356,711 - Pf1N1B4_303 0.43 +0.8 356,711 - Pf1N1B4_303 0.43 +0.9 356,773 + Pf1N1B4_303 0.48 +1.5 356,773 + Pf1N1B4_303 0.48 +1.2 357,061 + Pf1N1B4_303 0.68 -2.1 357,125 + -0.7 357,156 + Pf1N1B4_303 0.75 -0.1 357,156 + Pf1N1B4_303 0.75 -0.7 357,156 + Pf1N1B4_303 0.75 +0.9 357,156 + Pf1N1B4_303 0.75 +2.5 357,156 + Pf1N1B4_303 0.75 -0.5 357,156 + Pf1N1B4_303 0.75 -1.1 357,157 - Pf1N1B4_303 0.75 -0.8 357,157 - Pf1N1B4_303 0.75 -2.9 357,157 - Pf1N1B4_303 0.75 -0.7 357,157 - Pf1N1B4_303 0.75 +0.9 357,157 - Pf1N1B4_303 0.75 +1.2 357,277 + Pf1N1B4_303 0.83 +0.5 357,277 + Pf1N1B4_303 0.83 +1.1 357,277 + Pf1N1B4_303 0.83 -0.5 357,277 + Pf1N1B4_303 0.83 +0.2 357,277 + Pf1N1B4_303 0.83 +1.0 357,277 + Pf1N1B4_303 0.83 -1.1 357,277 + Pf1N1B4_303 0.83 +1.6 357,277 + Pf1N1B4_303 0.83 +1.1 357,278 - -0.7 357,304 + Pf1N1B4_303 0.85 +3.5 357,598 + -0.1 357,671 + +0.6 357,681 + -0.6 357,681 + -0.5 357,681 + -1.1 357,698 + -2.1 357,700 + +0.9 357,700 + -2.9 357,706 + -1.1 357,717 + +1.2 357,718 - +2.7 357,743 + -1.1 357,744 - -1.1 357,744 - -0.2 357,744 - -0.4 357,828 + +1.0 357,829 - +0.4 358,347 - Pf1N1B4_304 0.74 +0.3 358,384 - Pf1N1B4_304 0.78 +0.5 358,384 - Pf1N1B4_304 0.78 +0.8 358,441 - Pf1N1B4_304 0.85 -0.1
Or see this region's nucleotide sequence