Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2739 and Pf1N1B4_2740 are separated by 105 nucleotides Pf1N1B4_2740 and Pf1N1B4_2741 overlap by 4 nucleotides Pf1N1B4_2741 and Pf1N1B4_2742 are separated by 141 nucleotides
Pf1N1B4_2739 - acetyltransferase, GNAT family, at 3,078,266 to 3,078,835
_2739
Pf1N1B4_2740 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 3,078,941 to 3,079,810
_2740
Pf1N1B4_2741 - Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family, at 3,079,807 to 3,080,400
_2741
Pf1N1B4_2742 - putative exported protein, at 3,080,542 to 3,081,501
_2742
Position (kb)
3079
3080
3081 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3078.833 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.879 kb on + strand at 3079.057 kb on - strand, within Pf1N1B4_2740 at 3079.127 kb on + strand, within Pf1N1B4_2740 at 3079.128 kb on - strand, within Pf1N1B4_2740 at 3079.128 kb on - strand, within Pf1N1B4_2740 at 3079.166 kb on + strand, within Pf1N1B4_2740 at 3079.268 kb on + strand, within Pf1N1B4_2740 at 3079.311 kb on + strand, within Pf1N1B4_2740 at 3079.312 kb on - strand, within Pf1N1B4_2740 at 3079.312 kb on - strand, within Pf1N1B4_2740 at 3079.312 kb on - strand, within Pf1N1B4_2740 at 3079.445 kb on + strand, within Pf1N1B4_2740 at 3079.445 kb on + strand, within Pf1N1B4_2740 at 3079.446 kb on - strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.680 kb on - strand, within Pf1N1B4_2740 at 3080.118 kb on - strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.160 kb on - strand, within Pf1N1B4_2741 at 3080.160 kb on - strand, within Pf1N1B4_2741 at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.478 kb on + strand at 3080.580 kb on + strand at 3080.581 kb on - strand at 3080.727 kb on + strand, within Pf1N1B4_2742 at 3080.770 kb on + strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.834 kb on + strand, within Pf1N1B4_2742 at 3080.834 kb on + strand, within Pf1N1B4_2742 at 3080.835 kb on - strand, within Pf1N1B4_2742 at 3080.859 kb on - strand, within Pf1N1B4_2742 at 3080.859 kb on - strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.955 kb on - strand, within Pf1N1B4_2742 at 3080.955 kb on - strand, within Pf1N1B4_2742 at 3081.136 kb on - strand, within Pf1N1B4_2742 at 3081.188 kb on - strand, within Pf1N1B4_2742 at 3081.188 kb on - strand, within Pf1N1B4_2742 at 3081.308 kb on + strand, within Pf1N1B4_2742 at 3081.309 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 3,078,833 + -1.7 3,078,851 + -0.7 3,078,851 + -1.4 3,078,851 + +0.3 3,078,851 + +1.9 3,078,852 - +0.8 3,078,852 - -0.3 3,078,852 - +0.3 3,078,852 - -0.4 3,078,852 - -0.8 3,078,879 + -1.2 3,079,057 - Pf1N1B4_2740 0.13 +0.3 3,079,127 + Pf1N1B4_2740 0.21 +0.8 3,079,128 - Pf1N1B4_2740 0.21 -1.0 3,079,128 - Pf1N1B4_2740 0.21 +1.2 3,079,166 + Pf1N1B4_2740 0.26 +0.9 3,079,268 + Pf1N1B4_2740 0.38 +2.0 3,079,311 + Pf1N1B4_2740 0.43 -0.2 3,079,312 - Pf1N1B4_2740 0.43 -1.7 3,079,312 - Pf1N1B4_2740 0.43 -0.5 3,079,312 - Pf1N1B4_2740 0.43 +0.2 3,079,445 + Pf1N1B4_2740 0.58 +2.8 3,079,445 + Pf1N1B4_2740 0.58 +0.3 3,079,446 - Pf1N1B4_2740 0.58 +0.3 3,079,475 + Pf1N1B4_2740 0.61 +0.8 3,079,475 + Pf1N1B4_2740 0.61 -0.4 3,079,475 + Pf1N1B4_2740 0.61 +0.7 3,079,475 + Pf1N1B4_2740 0.61 +0.6 3,079,475 + Pf1N1B4_2740 0.61 -0.4 3,079,476 - Pf1N1B4_2740 0.61 -0.7 3,079,476 - Pf1N1B4_2740 0.61 -1.2 3,079,476 - Pf1N1B4_2740 0.61 +0.3 3,079,476 - Pf1N1B4_2740 0.61 -1.5 3,079,476 - Pf1N1B4_2740 0.61 -1.7 3,079,529 + Pf1N1B4_2740 0.68 +0.3 3,079,529 + Pf1N1B4_2740 0.68 +1.3 3,079,529 + Pf1N1B4_2740 0.68 +0.3 3,079,529 + Pf1N1B4_2740 0.68 +2.3 3,079,529 + Pf1N1B4_2740 0.68 -1.7 3,079,529 + Pf1N1B4_2740 0.68 -1.7 3,079,529 + Pf1N1B4_2740 0.68 +0.3 3,079,529 + Pf1N1B4_2740 0.68 -0.1 3,079,529 + Pf1N1B4_2740 0.68 -2.5 3,079,529 + Pf1N1B4_2740 0.68 +0.1 3,079,530 - Pf1N1B4_2740 0.68 -0.7 3,079,530 - Pf1N1B4_2740 0.68 +0.2 3,079,530 - Pf1N1B4_2740 0.68 +0.2 3,079,530 - Pf1N1B4_2740 0.68 +0.3 3,079,530 - Pf1N1B4_2740 0.68 +1.9 3,079,680 - Pf1N1B4_2740 0.85 +0.9 3,080,118 - Pf1N1B4_2741 0.52 +0.3 3,080,159 + Pf1N1B4_2741 0.59 -0.2 3,080,159 + Pf1N1B4_2741 0.59 -0.0 3,080,159 + Pf1N1B4_2741 0.59 +1.3 3,080,159 + Pf1N1B4_2741 0.59 -0.3 3,080,160 - Pf1N1B4_2741 0.59 +0.3 3,080,160 - Pf1N1B4_2741 0.59 +0.5 3,080,405 + -0.1 3,080,405 + -0.7 3,080,405 + +1.2 3,080,405 + +0.3 3,080,405 + +1.2 3,080,405 + +2.7 3,080,405 + -0.7 3,080,405 + -1.2 3,080,405 + -0.7 3,080,406 - +0.0 3,080,406 - -0.1 3,080,406 - -1.2 3,080,406 - +1.8 3,080,406 - -1.2 3,080,406 - -2.0 3,080,478 + +2.3 3,080,580 + +1.5 3,080,581 - -0.1 3,080,727 + Pf1N1B4_2742 0.19 -1.0 3,080,770 + Pf1N1B4_2742 0.24 -0.1 3,080,771 - Pf1N1B4_2742 0.24 +0.3 3,080,771 - Pf1N1B4_2742 0.24 +0.6 3,080,771 - Pf1N1B4_2742 0.24 -1.2 3,080,771 - Pf1N1B4_2742 0.24 -0.7 3,080,774 + Pf1N1B4_2742 0.24 +0.3 3,080,774 + Pf1N1B4_2742 0.24 +0.3 3,080,774 + Pf1N1B4_2742 0.24 +0.8 3,080,774 + Pf1N1B4_2742 0.24 +2.2 3,080,775 - Pf1N1B4_2742 0.24 +1.3 3,080,775 - Pf1N1B4_2742 0.24 -1.2 3,080,775 - Pf1N1B4_2742 0.24 -2.0 3,080,775 - Pf1N1B4_2742 0.24 +2.1 3,080,795 + Pf1N1B4_2742 0.26 -1.2 3,080,795 + Pf1N1B4_2742 0.26 +1.9 3,080,795 + Pf1N1B4_2742 0.26 -1.2 3,080,834 + Pf1N1B4_2742 0.30 +1.2 3,080,834 + Pf1N1B4_2742 0.30 -1.8 3,080,835 - Pf1N1B4_2742 0.31 +0.3 3,080,859 - Pf1N1B4_2742 0.33 +0.0 3,080,859 - Pf1N1B4_2742 0.33 +0.1 3,080,924 + Pf1N1B4_2742 0.40 -1.5 3,080,924 + Pf1N1B4_2742 0.40 +1.9 3,080,924 + Pf1N1B4_2742 0.40 +0.6 3,080,924 + Pf1N1B4_2742 0.40 +1.9 3,080,924 + Pf1N1B4_2742 0.40 +0.1 3,080,924 + Pf1N1B4_2742 0.40 -1.0 3,080,924 + Pf1N1B4_2742 0.40 -1.4 3,080,925 - Pf1N1B4_2742 0.40 +0.2 3,080,925 - Pf1N1B4_2742 0.40 -1.2 3,080,925 - Pf1N1B4_2742 0.40 +1.2 3,080,925 - Pf1N1B4_2742 0.40 +0.0 3,080,925 - Pf1N1B4_2742 0.40 -0.7 3,080,930 + Pf1N1B4_2742 0.40 -2.0 3,080,930 + Pf1N1B4_2742 0.40 +0.3 3,080,930 + Pf1N1B4_2742 0.40 +0.2 3,080,931 - Pf1N1B4_2742 0.41 +1.9 3,080,931 - Pf1N1B4_2742 0.41 +0.3 3,080,931 - Pf1N1B4_2742 0.41 +0.8 3,080,954 + Pf1N1B4_2742 0.43 +2.2 3,080,954 + Pf1N1B4_2742 0.43 -1.2 3,080,954 + Pf1N1B4_2742 0.43 +1.0 3,080,955 - Pf1N1B4_2742 0.43 -2.7 3,080,955 - Pf1N1B4_2742 0.43 -0.7 3,081,136 - Pf1N1B4_2742 0.62 -0.7 3,081,188 - Pf1N1B4_2742 0.67 -0.1 3,081,188 - Pf1N1B4_2742 0.67 -1.7 3,081,308 + Pf1N1B4_2742 0.80 +0.3 3,081,309 - +0.9
Or see this region's nucleotide sequence