Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2482 and Pf1N1B4_2483 are separated by 130 nucleotides Pf1N1B4_2483 and Pf1N1B4_2484 are separated by 24 nucleotides Pf1N1B4_2484 and Pf1N1B4_2485 overlap by 43 nucleotides Pf1N1B4_2485 and Pf1N1B4_2486 are separated by 114 nucleotides
Pf1N1B4_2482 - Putative phosphatase, at 2,816,126 to 2,818,027
_2482
Pf1N1B4_2483 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 2,818,158 to 2,818,922
_2483
Pf1N1B4_2484 - hypothetical protein, at 2,818,947 to 2,819,150
_2484
Pf1N1B4_2485 - Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy, at 2,819,108 to 2,819,743
_2485
Pf1N1B4_2486 - hypothetical protein, at 2,819,858 to 2,819,971
_2486
Position (kb)
2818
2819 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2817.220 kb on + strand, within Pf1N1B4_2482 at 2817.220 kb on + strand, within Pf1N1B4_2482 at 2817.280 kb on + strand, within Pf1N1B4_2482 at 2817.321 kb on + strand, within Pf1N1B4_2482 at 2817.321 kb on + strand, within Pf1N1B4_2482 at 2817.321 kb on + strand, within Pf1N1B4_2482 at 2817.321 kb on + strand, within Pf1N1B4_2482 at 2817.322 kb on - strand, within Pf1N1B4_2482 at 2817.322 kb on - strand, within Pf1N1B4_2482 at 2817.322 kb on - strand, within Pf1N1B4_2482 at 2817.322 kb on - strand, within Pf1N1B4_2482 at 2817.322 kb on - strand, within Pf1N1B4_2482 at 2817.580 kb on + strand at 2817.580 kb on + strand, within Pf1N1B4_2482 at 2817.581 kb on - strand, within Pf1N1B4_2482 at 2817.606 kb on + strand, within Pf1N1B4_2482 at 2817.606 kb on + strand, within Pf1N1B4_2482 at 2817.615 kb on + strand, within Pf1N1B4_2482 at 2817.616 kb on - strand, within Pf1N1B4_2482 at 2817.616 kb on - strand, within Pf1N1B4_2482 at 2817.616 kb on - strand, within Pf1N1B4_2482 at 2817.799 kb on - strand, within Pf1N1B4_2482 at 2818.016 kb on + strand at 2818.016 kb on + strand at 2818.016 kb on + strand at 2818.016 kb on + strand at 2818.016 kb on + strand at 2818.017 kb on - strand at 2818.017 kb on - strand at 2818.017 kb on - strand at 2818.019 kb on + strand at 2818.019 kb on + strand at 2818.019 kb on + strand at 2818.020 kb on - strand at 2818.020 kb on - strand at 2818.020 kb on - strand at 2818.020 kb on - strand at 2818.061 kb on - strand at 2818.061 kb on - strand at 2818.095 kb on + strand at 2818.096 kb on - strand at 2818.096 kb on - strand at 2818.134 kb on + strand at 2818.134 kb on + strand at 2818.135 kb on - strand at 2818.169 kb on + strand at 2818.423 kb on - strand, within Pf1N1B4_2483 at 2818.670 kb on - strand, within Pf1N1B4_2483 at 2818.670 kb on - strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.802 kb on - strand, within Pf1N1B4_2483 at 2818.802 kb on - strand, within Pf1N1B4_2483 at 2818.802 kb on - strand, within Pf1N1B4_2483 at 2818.910 kb on + strand at 2818.910 kb on + strand at 2818.911 kb on - strand at 2818.911 kb on - strand at 2819.033 kb on + strand, within Pf1N1B4_2484 at 2819.034 kb on - strand, within Pf1N1B4_2484 at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.104 kb on + strand at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.105 kb on - strand, within Pf1N1B4_2484 at 2819.105 kb on - strand, within Pf1N1B4_2484 at 2819.148 kb on + strand at 2819.149 kb on - strand at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.605 kb on - strand, within Pf1N1B4_2485 at 2819.605 kb on - strand, within Pf1N1B4_2485 at 2819.744 kb on - strand at 2819.783 kb on + strand at 2819.784 kb on - strand at 2819.784 kb on - strand at 2819.814 kb on + strand at 2819.826 kb on - strand at 2819.859 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.864 kb on - strand at 2819.864 kb on - strand at 2819.894 kb on - strand, within Pf1N1B4_2486 at 2819.922 kb on + strand, within Pf1N1B4_2486
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 2,817,220 + Pf1N1B4_2482 0.58 +1.7 2,817,220 + Pf1N1B4_2482 0.58 -1.6 2,817,280 + Pf1N1B4_2482 0.61 +2.0 2,817,321 + Pf1N1B4_2482 0.63 +2.0 2,817,321 + Pf1N1B4_2482 0.63 +3.4 2,817,321 + Pf1N1B4_2482 0.63 +2.2 2,817,321 + Pf1N1B4_2482 0.63 -0.3 2,817,322 - Pf1N1B4_2482 0.63 +0.2 2,817,322 - Pf1N1B4_2482 0.63 +1.0 2,817,322 - Pf1N1B4_2482 0.63 +1.2 2,817,322 - Pf1N1B4_2482 0.63 +1.2 2,817,322 - Pf1N1B4_2482 0.63 +1.8 2,817,580 + +0.4 2,817,580 + Pf1N1B4_2482 0.76 -0.6 2,817,581 - Pf1N1B4_2482 0.76 +1.7 2,817,606 + Pf1N1B4_2482 0.78 +1.4 2,817,606 + Pf1N1B4_2482 0.78 +0.3 2,817,615 + Pf1N1B4_2482 0.78 +0.4 2,817,616 - Pf1N1B4_2482 0.78 +1.4 2,817,616 - Pf1N1B4_2482 0.78 -1.6 2,817,616 - Pf1N1B4_2482 0.78 +0.6 2,817,799 - Pf1N1B4_2482 0.88 +1.4 2,818,016 + +0.1 2,818,016 + -0.0 2,818,016 + -1.9 2,818,016 + +0.4 2,818,016 + -1.0 2,818,017 - +1.7 2,818,017 - -0.8 2,818,017 - -0.4 2,818,019 + -0.6 2,818,019 + +0.4 2,818,019 + +1.4 2,818,020 - -0.2 2,818,020 - -1.2 2,818,020 - -0.6 2,818,020 - -0.7 2,818,061 - +0.1 2,818,061 - +0.4 2,818,095 + -1.4 2,818,096 - +0.8 2,818,096 - -0.9 2,818,134 + -0.6 2,818,134 + -0.1 2,818,135 - +0.2 2,818,169 + +1.0 2,818,423 - Pf1N1B4_2483 0.35 -0.2 2,818,670 - Pf1N1B4_2483 0.67 -0.6 2,818,670 - Pf1N1B4_2483 0.67 +0.7 2,818,801 + Pf1N1B4_2483 0.84 -1.1 2,818,801 + +0.4 2,818,801 + Pf1N1B4_2483 0.84 -0.3 2,818,801 + Pf1N1B4_2483 0.84 -0.0 2,818,801 + Pf1N1B4_2483 0.84 -1.2 2,818,801 + Pf1N1B4_2483 0.84 -1.2 2,818,802 - Pf1N1B4_2483 0.84 +0.1 2,818,802 - Pf1N1B4_2483 0.84 +0.4 2,818,802 - Pf1N1B4_2483 0.84 -1.9 2,818,910 + -0.3 2,818,910 + -1.9 2,818,911 - +1.6 2,818,911 - -1.6 2,819,033 + Pf1N1B4_2484 0.42 -1.2 2,819,034 - Pf1N1B4_2484 0.43 +0.7 2,819,104 + Pf1N1B4_2484 0.77 -1.9 2,819,104 + Pf1N1B4_2484 0.77 +0.7 2,819,104 + -0.6 2,819,104 + Pf1N1B4_2484 0.77 -1.2 2,819,105 - Pf1N1B4_2484 0.77 -0.6 2,819,105 - Pf1N1B4_2484 0.77 -0.3 2,819,148 + -1.9 2,819,149 - +0.4 2,819,604 + Pf1N1B4_2485 0.78 +2.3 2,819,604 + Pf1N1B4_2485 0.78 +0.2 2,819,604 + Pf1N1B4_2485 0.78 -1.0 2,819,605 - Pf1N1B4_2485 0.78 +0.9 2,819,605 - Pf1N1B4_2485 0.78 -1.6 2,819,744 - -0.2 2,819,783 + +2.4 2,819,784 - +1.7 2,819,784 - +2.0 2,819,814 + -0.6 2,819,826 - -0.6 2,819,859 + +0.7 2,819,863 + +0.8 2,819,863 + -0.6 2,819,863 + +0.4 2,819,863 + -1.4 2,819,863 + +0.4 2,819,863 + -1.6 2,819,863 + -1.6 2,819,863 + +1.0 2,819,863 + -1.4 2,819,864 - +0.1 2,819,864 - -0.8 2,819,894 - Pf1N1B4_2486 0.32 +0.4 2,819,922 + Pf1N1B4_2486 0.56 -0.6
Or see this region's nucleotide sequence