Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2480 and Pf1N1B4_2481 are separated by 162 nucleotides Pf1N1B4_2481 and Pf1N1B4_2482 are separated by 194 nucleotides
Pf1N1B4_2480 - Protein rarD, at 2,813,907 to 2,814,794
_2480
Pf1N1B4_2481 - YihE protein, required for LPS synthesis, at 2,814,957 to 2,815,931
_2481
Pf1N1B4_2482 - Putative phosphatase, at 2,816,126 to 2,818,027
_2482
Position (kb)
2814
2815
2816 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2813.969 kb on + strand at 2813.969 kb on + strand at 2813.969 kb on + strand at 2813.970 kb on - strand at 2814.056 kb on - strand, within Pf1N1B4_2480 at 2814.056 kb on - strand, within Pf1N1B4_2480 at 2814.056 kb on - strand, within Pf1N1B4_2480 at 2814.079 kb on + strand, within Pf1N1B4_2480 at 2814.079 kb on + strand, within Pf1N1B4_2480 at 2814.080 kb on - strand, within Pf1N1B4_2480 at 2814.305 kb on - strand at 2814.305 kb on - strand, within Pf1N1B4_2480 at 2814.305 kb on - strand, within Pf1N1B4_2480 at 2814.363 kb on + strand, within Pf1N1B4_2480 at 2814.364 kb on - strand, within Pf1N1B4_2480 at 2814.364 kb on - strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.479 kb on - strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.527 kb on - strand at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.719 kb on - strand at 2814.719 kb on - strand at 2814.719 kb on - strand at 2814.719 kb on - strand at 2814.719 kb on - strand at 2814.719 kb on - strand at 2814.745 kb on + strand at 2814.745 kb on + strand at 2814.745 kb on + strand at 2814.745 kb on + strand at 2814.745 kb on + strand at 2814.746 kb on - strand at 2814.746 kb on - strand at 2814.746 kb on - strand at 2814.766 kb on + strand at 2814.809 kb on - strand at 2814.833 kb on + strand at 2814.833 kb on + strand at 2814.833 kb on + strand at 2814.833 kb on + strand at 2814.833 kb on + strand at 2814.833 kb on + strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.856 kb on - strand at 2814.941 kb on - strand at 2814.959 kb on - strand at 2814.959 kb on - strand at 2815.106 kb on - strand, within Pf1N1B4_2481 at 2815.106 kb on - strand, within Pf1N1B4_2481 at 2815.106 kb on - strand, within Pf1N1B4_2481 at 2815.180 kb on + strand, within Pf1N1B4_2481 at 2815.180 kb on + strand, within Pf1N1B4_2481 at 2815.248 kb on - strand, within Pf1N1B4_2481 at 2815.248 kb on - strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.376 kb on - strand, within Pf1N1B4_2481 at 2815.376 kb on - strand, within Pf1N1B4_2481 at 2815.574 kb on - strand, within Pf1N1B4_2481 at 2815.831 kb on + strand, within Pf1N1B4_2481 at 2815.831 kb on + strand, within Pf1N1B4_2481 at 2816.411 kb on - strand, within Pf1N1B4_2482 at 2816.692 kb on - strand, within Pf1N1B4_2482 at 2816.692 kb on - strand, within Pf1N1B4_2482 at 2816.692 kb on - strand, within Pf1N1B4_2482 at 2816.692 kb on - strand, within Pf1N1B4_2482 at 2816.747 kb on - strand, within Pf1N1B4_2482 at 2816.747 kb on - strand, within Pf1N1B4_2482 at 2816.913 kb on + strand, within Pf1N1B4_2482 at 2816.913 kb on + strand, within Pf1N1B4_2482 at 2816.914 kb on - strand, within Pf1N1B4_2482 at 2816.914 kb on - strand, within Pf1N1B4_2482 at 2816.914 kb on - strand, within Pf1N1B4_2482 at 2816.914 kb on - strand, within Pf1N1B4_2482 at 2816.914 kb on - strand, within Pf1N1B4_2482
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 2,813,969 + -2.9 2,813,969 + +1.4 2,813,969 + +0.4 2,813,970 - -0.0 2,814,056 - Pf1N1B4_2480 0.17 -1.2 2,814,056 - Pf1N1B4_2480 0.17 +2.7 2,814,056 - Pf1N1B4_2480 0.17 +1.7 2,814,079 + Pf1N1B4_2480 0.19 -0.6 2,814,079 + Pf1N1B4_2480 0.19 +0.4 2,814,080 - Pf1N1B4_2480 0.19 +0.2 2,814,305 - -1.6 2,814,305 - Pf1N1B4_2480 0.45 +0.6 2,814,305 - Pf1N1B4_2480 0.45 +0.7 2,814,363 + Pf1N1B4_2480 0.51 +0.2 2,814,364 - Pf1N1B4_2480 0.51 -0.9 2,814,364 - Pf1N1B4_2480 0.51 -1.0 2,814,475 + Pf1N1B4_2480 0.64 -0.6 2,814,475 + Pf1N1B4_2480 0.64 -0.8 2,814,475 + Pf1N1B4_2480 0.64 -1.2 2,814,475 + Pf1N1B4_2480 0.64 +0.7 2,814,475 + Pf1N1B4_2480 0.64 -0.6 2,814,476 - Pf1N1B4_2480 0.64 +1.4 2,814,476 - Pf1N1B4_2480 0.64 +1.7 2,814,476 - Pf1N1B4_2480 0.64 +2.2 2,814,476 - Pf1N1B4_2480 0.64 +1.0 2,814,476 - Pf1N1B4_2480 0.64 +0.1 2,814,476 - Pf1N1B4_2480 0.64 -0.6 2,814,476 - Pf1N1B4_2480 0.64 -1.2 2,814,479 - Pf1N1B4_2480 0.64 +1.7 2,814,526 + Pf1N1B4_2480 0.70 +0.8 2,814,526 + Pf1N1B4_2480 0.70 -0.9 2,814,526 + Pf1N1B4_2480 0.70 +0.4 2,814,526 + Pf1N1B4_2480 0.70 +1.0 2,814,526 + Pf1N1B4_2480 0.70 +0.4 2,814,526 + Pf1N1B4_2480 0.70 +1.0 2,814,526 + Pf1N1B4_2480 0.70 -1.8 2,814,526 + Pf1N1B4_2480 0.70 -0.6 2,814,526 + Pf1N1B4_2480 0.70 -0.6 2,814,526 + Pf1N1B4_2480 0.70 -0.3 2,814,527 - +0.4 2,814,527 - Pf1N1B4_2480 0.70 +0.1 2,814,527 - Pf1N1B4_2480 0.70 +1.2 2,814,527 - Pf1N1B4_2480 0.70 -0.9 2,814,527 - Pf1N1B4_2480 0.70 +0.2 2,814,527 - Pf1N1B4_2480 0.70 +1.3 2,814,527 - Pf1N1B4_2480 0.70 +1.4 2,814,527 - Pf1N1B4_2480 0.70 +0.8 2,814,527 - Pf1N1B4_2480 0.70 +1.4 2,814,527 - Pf1N1B4_2480 0.70 +0.3 2,814,527 - Pf1N1B4_2480 0.70 -0.6 2,814,719 - -0.6 2,814,719 - -2.4 2,814,719 - -1.3 2,814,719 - +0.1 2,814,719 - -0.2 2,814,719 - +0.8 2,814,745 + +0.4 2,814,745 + -1.9 2,814,745 + +1.0 2,814,745 + -0.9 2,814,745 + +0.4 2,814,746 - +1.2 2,814,746 - +0.4 2,814,746 - +0.4 2,814,766 + -0.2 2,814,809 - +0.7 2,814,833 + -1.9 2,814,833 + -0.2 2,814,833 + +0.7 2,814,833 + -0.6 2,814,833 + +3.0 2,814,833 + -1.6 2,814,834 - -0.9 2,814,834 - -0.0 2,814,834 - -1.2 2,814,834 - +0.4 2,814,834 - -1.2 2,814,834 - -0.1 2,814,834 - -0.6 2,814,834 - +1.3 2,814,834 - +2.2 2,814,834 - +1.4 2,814,835 + -0.0 2,814,835 + -0.6 2,814,835 + -0.3 2,814,835 + +0.6 2,814,835 + +0.4 2,814,835 + +1.4 2,814,835 + +0.8 2,814,835 + +0.1 2,814,836 - +0.3 2,814,836 - +1.6 2,814,836 - -1.3 2,814,836 - +1.5 2,814,836 - +0.6 2,814,836 - +1.1 2,814,836 - -1.8 2,814,836 - -0.6 2,814,836 - -1.6 2,814,836 - +0.1 2,814,836 - +1.4 2,814,836 - -1.9 2,814,836 - +0.4 2,814,856 - +2.0 2,814,941 - +1.4 2,814,959 - +1.1 2,814,959 - -0.6 2,815,106 - Pf1N1B4_2481 0.15 -1.6 2,815,106 - Pf1N1B4_2481 0.15 -0.2 2,815,106 - Pf1N1B4_2481 0.15 +0.4 2,815,180 + Pf1N1B4_2481 0.23 -1.2 2,815,180 + Pf1N1B4_2481 0.23 +1.0 2,815,248 - Pf1N1B4_2481 0.30 +1.8 2,815,248 - Pf1N1B4_2481 0.30 -1.6 2,815,375 + Pf1N1B4_2481 0.43 -0.3 2,815,375 + Pf1N1B4_2481 0.43 +0.1 2,815,375 + Pf1N1B4_2481 0.43 +0.4 2,815,375 + Pf1N1B4_2481 0.43 -0.2 2,815,375 + Pf1N1B4_2481 0.43 +0.4 2,815,375 + Pf1N1B4_2481 0.43 +1.0 2,815,375 + Pf1N1B4_2481 0.43 -1.6 2,815,376 - Pf1N1B4_2481 0.43 -1.2 2,815,376 - Pf1N1B4_2481 0.43 +1.6 2,815,574 - Pf1N1B4_2481 0.63 +0.7 2,815,831 + Pf1N1B4_2481 0.90 -1.9 2,815,831 + Pf1N1B4_2481 0.90 +0.3 2,816,411 - Pf1N1B4_2482 0.15 +1.7 2,816,692 - Pf1N1B4_2482 0.30 -0.4 2,816,692 - Pf1N1B4_2482 0.30 -2.4 2,816,692 - Pf1N1B4_2482 0.30 +0.0 2,816,692 - Pf1N1B4_2482 0.30 +0.4 2,816,747 - Pf1N1B4_2482 0.33 +1.4 2,816,747 - Pf1N1B4_2482 0.33 +0.8 2,816,913 + Pf1N1B4_2482 0.41 +1.2 2,816,913 + Pf1N1B4_2482 0.41 +1.7 2,816,914 - Pf1N1B4_2482 0.41 -1.0 2,816,914 - Pf1N1B4_2482 0.41 -0.3 2,816,914 - Pf1N1B4_2482 0.41 +0.7 2,816,914 - Pf1N1B4_2482 0.41 -0.3 2,816,914 - Pf1N1B4_2482 0.41 -3.0
Or see this region's nucleotide sequence