Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2422 and Pf1N1B4_2423 are separated by 437 nucleotides Pf1N1B4_2423 and Pf1N1B4_2424 are separated by 443 nucleotides Pf1N1B4_2424 and Pf1N1B4_2425 overlap by 27 nucleotides
Pf1N1B4_2422 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 2,747,220 to 2,748,170
_2422
Pf1N1B4_2423 - L-serine dehydratase (EC 4.3.1.17), at 2,748,608 to 2,749,984
_2423
Pf1N1B4_2424 - hypothetical protein, at 2,750,428 to 2,750,544
_2424
Pf1N1B4_2425 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,750,518 to 2,751,621
_2425
Position (kb)
2748
2749
2750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2747.737 kb on + strand, within Pf1N1B4_2422 at 2747.737 kb on + strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.802 kb on - strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.830 kb on + strand, within Pf1N1B4_2422 at 2747.831 kb on - strand, within Pf1N1B4_2422 at 2747.849 kb on + strand, within Pf1N1B4_2422 at 2747.922 kb on - strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.966 kb on - strand, within Pf1N1B4_2422 at 2747.966 kb on - strand, within Pf1N1B4_2422 at 2748.004 kb on + strand, within Pf1N1B4_2422 at 2748.005 kb on - strand, within Pf1N1B4_2422 at 2748.170 kb on + strand at 2748.226 kb on + strand at 2748.226 kb on + strand at 2748.226 kb on + strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.300 kb on + strand at 2748.300 kb on + strand at 2748.300 kb on + strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.322 kb on - strand at 2748.379 kb on + strand at 2748.380 kb on - strand at 2748.380 kb on - strand at 2748.380 kb on - strand at 2748.547 kb on - strand at 2748.758 kb on + strand, within Pf1N1B4_2423 at 2748.758 kb on + strand, within Pf1N1B4_2423 at 2748.759 kb on - strand, within Pf1N1B4_2423 at 2748.759 kb on - strand, within Pf1N1B4_2423 at 2748.759 kb on - strand, within Pf1N1B4_2423 at 2748.897 kb on + strand, within Pf1N1B4_2423 at 2748.898 kb on - strand, within Pf1N1B4_2423 at 2748.898 kb on - strand, within Pf1N1B4_2423 at 2748.898 kb on - strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.037 kb on + strand, within Pf1N1B4_2423 at 2749.037 kb on + strand, within Pf1N1B4_2423 at 2749.037 kb on + strand, within Pf1N1B4_2423 at 2749.038 kb on - strand, within Pf1N1B4_2423 at 2749.038 kb on - strand, within Pf1N1B4_2423 at 2749.038 kb on - strand, within Pf1N1B4_2423 at 2749.118 kb on + strand, within Pf1N1B4_2423 at 2749.514 kb on + strand, within Pf1N1B4_2423 at 2749.514 kb on + strand, within Pf1N1B4_2423 at 2749.514 kb on + strand, within Pf1N1B4_2423 at 2749.515 kb on - strand, within Pf1N1B4_2423 at 2749.562 kb on + strand, within Pf1N1B4_2423 at 2749.562 kb on + strand, within Pf1N1B4_2423 at 2749.563 kb on - strand, within Pf1N1B4_2423 at 2749.744 kb on + strand, within Pf1N1B4_2423 at 2749.744 kb on + strand, within Pf1N1B4_2423 at 2749.745 kb on - strand, within Pf1N1B4_2423 at 2749.745 kb on - strand, within Pf1N1B4_2423 at 2749.745 kb on - strand at 2749.873 kb on + strand at 2749.873 kb on + strand at 2749.873 kb on + strand at 2749.873 kb on + strand at 2749.874 kb on - strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.935 kb on - strand at 2749.935 kb on - strand at 2749.935 kb on - strand at 2749.935 kb on - strand at 2749.936 kb on + strand at 2749.936 kb on + strand at 2749.937 kb on - strand at 2749.937 kb on - strand at 2749.993 kb on + strand at 2749.994 kb on - strand at 2750.055 kb on + strand at 2750.056 kb on - strand at 2750.056 kb on - strand at 2750.146 kb on - strand at 2750.146 kb on - strand at 2750.163 kb on + strand at 2750.163 kb on + strand at 2750.163 kb on + strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.325 kb on + strand at 2750.325 kb on + strand at 2750.325 kb on + strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.335 kb on + strand at 2750.354 kb on + strand at 2750.354 kb on + strand at 2750.355 kb on - strand at 2750.355 kb on - strand at 2750.385 kb on - strand at 2750.405 kb on + strand at 2750.430 kb on - strand at 2750.430 kb on - strand at 2750.465 kb on - strand, within Pf1N1B4_2424 at 2750.511 kb on + strand, within Pf1N1B4_2424 at 2750.601 kb on + strand at 2750.601 kb on + strand at 2750.601 kb on + strand at 2750.602 kb on - strand at 2750.621 kb on + strand at 2750.621 kb on + strand at 2750.621 kb on + strand at 2750.622 kb on - strand at 2750.622 kb on - strand at 2750.802 kb on + strand, within Pf1N1B4_2425 at 2750.803 kb on - strand, within Pf1N1B4_2425 at 2750.803 kb on - strand, within Pf1N1B4_2425
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 2,747,737 + Pf1N1B4_2422 0.54 +1.6 2,747,737 + Pf1N1B4_2422 0.54 +1.0 2,747,738 - Pf1N1B4_2422 0.54 -1.9 2,747,738 - Pf1N1B4_2422 0.54 +0.1 2,747,738 - Pf1N1B4_2422 0.54 +2.0 2,747,802 - Pf1N1B4_2422 0.61 -0.6 2,747,803 + Pf1N1B4_2422 0.61 -0.6 2,747,803 + Pf1N1B4_2422 0.61 -1.2 2,747,803 + Pf1N1B4_2422 0.61 +0.8 2,747,803 + Pf1N1B4_2422 0.61 +0.6 2,747,803 + Pf1N1B4_2422 0.61 -2.6 2,747,803 + Pf1N1B4_2422 0.61 +0.4 2,747,803 + Pf1N1B4_2422 0.61 +0.7 2,747,804 - Pf1N1B4_2422 0.61 -0.0 2,747,804 - Pf1N1B4_2422 0.61 +0.8 2,747,804 - Pf1N1B4_2422 0.61 +0.4 2,747,804 - Pf1N1B4_2422 0.61 +0.4 2,747,804 - Pf1N1B4_2422 0.61 -1.4 2,747,804 - Pf1N1B4_2422 0.61 +1.0 2,747,830 + Pf1N1B4_2422 0.64 -0.9 2,747,831 - Pf1N1B4_2422 0.64 -2.4 2,747,849 + Pf1N1B4_2422 0.66 +1.2 2,747,922 - Pf1N1B4_2422 0.74 +1.2 2,747,965 + Pf1N1B4_2422 0.78 +1.4 2,747,965 + Pf1N1B4_2422 0.78 +0.4 2,747,965 + Pf1N1B4_2422 0.78 -0.6 2,747,966 - Pf1N1B4_2422 0.78 +0.4 2,747,966 - Pf1N1B4_2422 0.78 -0.9 2,748,004 + Pf1N1B4_2422 0.82 +0.4 2,748,005 - Pf1N1B4_2422 0.83 -1.2 2,748,170 + +1.1 2,748,226 + +0.8 2,748,226 + -0.1 2,748,226 + +0.2 2,748,227 - -1.2 2,748,227 - -1.0 2,748,227 - +0.1 2,748,227 - -0.3 2,748,227 - -0.7 2,748,227 - -2.6 2,748,227 - -0.1 2,748,227 - +0.6 2,748,227 - +0.9 2,748,300 + +0.4 2,748,300 + +1.2 2,748,300 + -2.6 2,748,301 - -0.9 2,748,301 - +0.7 2,748,301 - -0.2 2,748,301 - -1.6 2,748,301 - -1.9 2,748,301 - -2.2 2,748,301 - +1.0 2,748,301 - +1.5 2,748,301 - +0.7 2,748,301 - +0.4 2,748,301 - +1.0 2,748,301 - -1.2 2,748,301 - +0.4 2,748,322 - +1.5 2,748,379 + +1.4 2,748,380 - -0.4 2,748,380 - +0.2 2,748,380 - -0.6 2,748,547 - -2.2 2,748,758 + Pf1N1B4_2423 0.11 +1.4 2,748,758 + Pf1N1B4_2423 0.11 +0.4 2,748,759 - Pf1N1B4_2423 0.11 -0.8 2,748,759 - Pf1N1B4_2423 0.11 -0.6 2,748,759 - Pf1N1B4_2423 0.11 -0.9 2,748,897 + Pf1N1B4_2423 0.21 +0.4 2,748,898 - Pf1N1B4_2423 0.21 +0.7 2,748,898 - Pf1N1B4_2423 0.21 +0.7 2,748,898 - Pf1N1B4_2423 0.21 +0.6 2,749,034 + Pf1N1B4_2423 0.31 -1.6 2,749,034 + Pf1N1B4_2423 0.31 +0.1 2,749,034 + Pf1N1B4_2423 0.31 -0.6 2,749,034 + Pf1N1B4_2423 0.31 +0.5 2,749,035 - Pf1N1B4_2423 0.31 -2.4 2,749,035 - Pf1N1B4_2423 0.31 -2.2 2,749,035 - Pf1N1B4_2423 0.31 +0.4 2,749,035 - Pf1N1B4_2423 0.31 -0.6 2,749,035 - Pf1N1B4_2423 0.31 -0.0 2,749,035 - Pf1N1B4_2423 0.31 +0.4 2,749,035 - Pf1N1B4_2423 0.31 +0.4 2,749,035 - Pf1N1B4_2423 0.31 +0.8 2,749,035 - Pf1N1B4_2423 0.31 -1.2 2,749,037 + Pf1N1B4_2423 0.31 +0.6 2,749,037 + Pf1N1B4_2423 0.31 -0.8 2,749,037 + Pf1N1B4_2423 0.31 -1.6 2,749,038 - Pf1N1B4_2423 0.31 -0.5 2,749,038 - Pf1N1B4_2423 0.31 -1.2 2,749,038 - Pf1N1B4_2423 0.31 +0.1 2,749,118 + Pf1N1B4_2423 0.37 +0.6 2,749,514 + Pf1N1B4_2423 0.66 +0.6 2,749,514 + Pf1N1B4_2423 0.66 +0.4 2,749,514 + Pf1N1B4_2423 0.66 -0.6 2,749,515 - Pf1N1B4_2423 0.66 +0.1 2,749,562 + Pf1N1B4_2423 0.69 -0.7 2,749,562 + Pf1N1B4_2423 0.69 -0.7 2,749,563 - Pf1N1B4_2423 0.69 -0.0 2,749,744 + Pf1N1B4_2423 0.82 +0.3 2,749,744 + Pf1N1B4_2423 0.82 +0.9 2,749,745 - Pf1N1B4_2423 0.83 +0.8 2,749,745 - Pf1N1B4_2423 0.83 -0.8 2,749,745 - -0.6 2,749,873 + +0.6 2,749,873 + +1.1 2,749,873 + -2.4 2,749,873 + -1.9 2,749,874 - +0.4 2,749,934 + -0.2 2,749,934 + +0.1 2,749,934 + +0.1 2,749,934 + -2.8 2,749,934 + +0.4 2,749,934 + -1.2 2,749,935 - -0.6 2,749,935 - -0.6 2,749,935 - +0.6 2,749,935 - -0.6 2,749,936 + -1.6 2,749,936 + -1.2 2,749,937 - +0.4 2,749,937 - +1.4 2,749,993 + -1.2 2,749,994 - -1.2 2,750,055 + +1.7 2,750,056 - +0.8 2,750,056 - +2.7 2,750,146 - -0.2 2,750,146 - -0.8 2,750,163 + +2.0 2,750,163 + +0.4 2,750,163 + -1.2 2,750,164 - +0.4 2,750,164 - +1.1 2,750,164 - -0.1 2,750,164 - -0.8 2,750,164 - -2.2 2,750,164 - +0.4 2,750,325 + +0.1 2,750,325 + -0.2 2,750,325 + +2.7 2,750,326 - -0.9 2,750,326 - -1.2 2,750,326 - -0.0 2,750,326 - -0.6 2,750,326 - +0.4 2,750,335 + +1.6 2,750,354 + -0.6 2,750,354 + +1.1 2,750,355 - -0.6 2,750,355 - -0.6 2,750,385 - +0.8 2,750,405 + -0.3 2,750,430 - -1.2 2,750,430 - -0.2 2,750,465 - Pf1N1B4_2424 0.32 +0.4 2,750,511 + Pf1N1B4_2424 0.71 +0.7 2,750,601 + -0.2 2,750,601 + -0.6 2,750,601 + -0.5 2,750,602 - +0.4 2,750,621 + -0.6 2,750,621 + +0.7 2,750,621 + +0.4 2,750,622 - +0.6 2,750,622 - -0.2 2,750,802 + Pf1N1B4_2425 0.26 +0.8 2,750,803 - Pf1N1B4_2425 0.26 +0.1 2,750,803 - Pf1N1B4_2425 0.26 -0.6
Or see this region's nucleotide sequence