Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2309 and Pf1N1B4_2310 are separated by 54 nucleotides Pf1N1B4_2310 and Pf1N1B4_2311 are separated by 114 nucleotides Pf1N1B4_2311 and Pf1N1B4_2312 are separated by 148 nucleotides Pf1N1B4_2312 and Pf1N1B4_2313 are separated by 115 nucleotides
Pf1N1B4_2309 - Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23), at 2,630,376 to 2,631,188
_2309
Pf1N1B4_2310 - Cation/multidrug efflux pump, at 2,631,243 to 2,631,566
_2310
Pf1N1B4_2311 - 4Fe-4S ferredoxin, iron-sulfur binding, at 2,631,681 to 2,631,932
_2311
Pf1N1B4_2312 - Phosphopantetheine adenylyltransferase (EC 2.7.7.3), at 2,632,081 to 2,632,560
_2312
Pf1N1B4_2313 - FIG022869: Oxidoreductase, GMC family, at 2,632,676 to 2,634,271
_2313
Position (kb)
2631
2632 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2630.692 kb on - strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.788 kb on + strand, within Pf1N1B4_2309 at 2630.789 kb on - strand, within Pf1N1B4_2309 at 2630.789 kb on - strand at 2630.789 kb on - strand, within Pf1N1B4_2309 at 2630.839 kb on + strand, within Pf1N1B4_2309 at 2630.885 kb on + strand, within Pf1N1B4_2309 at 2630.885 kb on + strand, within Pf1N1B4_2309 at 2630.885 kb on + strand, within Pf1N1B4_2309 at 2630.885 kb on + strand, within Pf1N1B4_2309 at 2630.885 kb on + strand, within Pf1N1B4_2309 at 2630.890 kb on - strand, within Pf1N1B4_2309 at 2630.961 kb on + strand, within Pf1N1B4_2309 at 2630.962 kb on - strand, within Pf1N1B4_2309 at 2631.063 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.079 kb on + strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.080 kb on - strand, within Pf1N1B4_2309 at 2631.087 kb on + strand at 2631.087 kb on + strand, within Pf1N1B4_2309 at 2631.196 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.203 kb on + strand at 2631.204 kb on - strand at 2631.204 kb on - strand at 2631.204 kb on - strand at 2631.204 kb on - strand at 2631.204 kb on - strand at 2631.204 kb on - strand at 2631.205 kb on + strand at 2631.205 kb on + strand at 2631.571 kb on + strand at 2631.571 kb on + strand at 2631.571 kb on + strand at 2631.571 kb on + strand at 2631.571 kb on + strand at 2631.571 kb on + strand at 2631.599 kb on + strand at 2631.600 kb on - strand at 2631.600 kb on - strand at 2631.689 kb on + strand at 2631.707 kb on - strand, within Pf1N1B4_2311 at 2631.707 kb on - strand, within Pf1N1B4_2311 at 2631.936 kb on - strand at 2631.969 kb on - strand at 2632.056 kb on - strand at 2632.082 kb on + strand at 2632.082 kb on + strand at 2632.089 kb on + strand at 2632.649 kb on + strand at 2632.649 kb on + strand at 2632.650 kb on - strand at 2632.651 kb on + strand at 2632.651 kb on + strand at 2632.651 kb on + strand at 2632.651 kb on + strand at 2632.652 kb on - strand at 2632.652 kb on - strand at 2632.664 kb on + strand at 2632.665 kb on - strand at 2632.665 kb on - strand at 2632.902 kb on + strand, within Pf1N1B4_2313
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 2,630,692 - Pf1N1B4_2309 0.39 +0.5 2,630,788 + Pf1N1B4_2309 0.51 -0.1 2,630,788 + Pf1N1B4_2309 0.51 -0.8 2,630,788 + Pf1N1B4_2309 0.51 -1.1 2,630,788 + Pf1N1B4_2309 0.51 -0.9 2,630,788 + Pf1N1B4_2309 0.51 +0.9 2,630,788 + Pf1N1B4_2309 0.51 +0.3 2,630,789 - Pf1N1B4_2309 0.51 -0.1 2,630,789 - +0.5 2,630,789 - Pf1N1B4_2309 0.51 +2.3 2,630,839 + Pf1N1B4_2309 0.57 +0.9 2,630,885 + Pf1N1B4_2309 0.63 +1.9 2,630,885 + Pf1N1B4_2309 0.63 -1.1 2,630,885 + Pf1N1B4_2309 0.63 -0.2 2,630,885 + Pf1N1B4_2309 0.63 -1.1 2,630,885 + Pf1N1B4_2309 0.63 +1.8 2,630,890 - Pf1N1B4_2309 0.63 -0.9 2,630,961 + Pf1N1B4_2309 0.72 +0.5 2,630,962 - Pf1N1B4_2309 0.72 -1.9 2,631,063 + Pf1N1B4_2309 0.85 -2.3 2,631,079 + Pf1N1B4_2309 0.86 +1.2 2,631,079 + Pf1N1B4_2309 0.86 -0.5 2,631,079 + Pf1N1B4_2309 0.86 +0.7 2,631,079 + Pf1N1B4_2309 0.86 -1.1 2,631,079 + Pf1N1B4_2309 0.86 +0.9 2,631,079 + Pf1N1B4_2309 0.86 +1.8 2,631,079 + Pf1N1B4_2309 0.86 +0.5 2,631,079 + Pf1N1B4_2309 0.86 -0.1 2,631,079 + Pf1N1B4_2309 0.86 +1.3 2,631,080 - Pf1N1B4_2309 0.87 -0.5 2,631,080 - Pf1N1B4_2309 0.87 +0.9 2,631,080 - Pf1N1B4_2309 0.87 +1.5 2,631,080 - Pf1N1B4_2309 0.87 +0.2 2,631,080 - Pf1N1B4_2309 0.87 +1.0 2,631,080 - Pf1N1B4_2309 0.87 +1.6 2,631,080 - Pf1N1B4_2309 0.87 +1.1 2,631,087 + +0.5 2,631,087 + Pf1N1B4_2309 0.87 -1.1 2,631,196 + +3.1 2,631,203 + +0.5 2,631,203 + +1.9 2,631,203 + -1.9 2,631,203 + +1.1 2,631,203 + +0.8 2,631,203 + +0.5 2,631,203 + -0.0 2,631,204 - -1.1 2,631,204 - -0.5 2,631,204 - -0.5 2,631,204 - +0.2 2,631,204 - +0.5 2,631,204 - +0.1 2,631,205 + -1.1 2,631,205 + +0.1 2,631,571 + +0.7 2,631,571 + +0.5 2,631,571 + +2.8 2,631,571 + +0.3 2,631,571 + -1.9 2,631,571 + +2.1 2,631,599 + +1.1 2,631,600 - +1.5 2,631,600 - +0.5 2,631,689 + +0.5 2,631,707 - Pf1N1B4_2311 0.10 +1.2 2,631,707 - Pf1N1B4_2311 0.10 +2.3 2,631,936 - +0.6 2,631,969 - -2.2 2,632,056 - -0.1 2,632,082 + -0.5 2,632,082 + -0.1 2,632,089 + -1.9 2,632,649 + +0.5 2,632,649 + +1.5 2,632,650 - -0.1 2,632,651 + -3.0 2,632,651 + +1.0 2,632,651 + -2.3 2,632,651 + -2.1 2,632,652 - -0.5 2,632,652 - -0.3 2,632,664 + +0.3 2,632,665 - +2.5 2,632,665 - -0.3 2,632,902 + Pf1N1B4_2313 0.14 +0.5
Or see this region's nucleotide sequence