Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2234 and Pf1N1B4_2235 are separated by 50 nucleotides Pf1N1B4_2235 and Pf1N1B4_2236 are separated by 269 nucleotides
Pf1N1B4_2234 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 2,547,299 to 2,548,132
_2234
Pf1N1B4_2235 - Glycine cleavage system H protein, at 2,548,183 to 2,548,569
_2235
Pf1N1B4_2236 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 2,548,839 to 2,551,712
_2236
Position (kb)
2548
2549 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2547.196 kb on + strand at 2547.252 kb on - strand at 2547.255 kb on + strand at 2547.256 kb on - strand at 2547.303 kb on - strand at 2547.303 kb on - strand at 2547.354 kb on - strand at 2547.563 kb on + strand, within Pf1N1B4_2234 at 2547.564 kb on - strand, within Pf1N1B4_2234 at 2547.691 kb on + strand, within Pf1N1B4_2234 at 2547.692 kb on - strand, within Pf1N1B4_2234 at 2547.879 kb on + strand, within Pf1N1B4_2234 at 2547.879 kb on + strand, within Pf1N1B4_2234 at 2547.982 kb on + strand, within Pf1N1B4_2234 at 2547.983 kb on - strand, within Pf1N1B4_2234 at 2547.983 kb on - strand, within Pf1N1B4_2234 at 2547.983 kb on - strand, within Pf1N1B4_2234 at 2547.983 kb on - strand, within Pf1N1B4_2234 at 2547.994 kb on + strand, within Pf1N1B4_2234 at 2547.994 kb on + strand, within Pf1N1B4_2234 at 2547.994 kb on + strand, within Pf1N1B4_2234 at 2548.001 kb on - strand, within Pf1N1B4_2234 at 2548.027 kb on - strand, within Pf1N1B4_2234 at 2548.131 kb on - strand at 2548.238 kb on + strand, within Pf1N1B4_2235 at 2548.238 kb on + strand, within Pf1N1B4_2235 at 2548.239 kb on - strand, within Pf1N1B4_2235 at 2548.239 kb on - strand, within Pf1N1B4_2235 at 2548.266 kb on + strand, within Pf1N1B4_2235 at 2548.266 kb on + strand, within Pf1N1B4_2235 at 2548.266 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.394 kb on - strand, within Pf1N1B4_2235 at 2548.394 kb on - strand, within Pf1N1B4_2235 at 2548.394 kb on - strand, within Pf1N1B4_2235 at 2548.471 kb on + strand, within Pf1N1B4_2235 at 2548.471 kb on + strand, within Pf1N1B4_2235 at 2548.471 kb on + strand, within Pf1N1B4_2235 at 2548.471 kb on + strand, within Pf1N1B4_2235 at 2548.471 kb on + strand, within Pf1N1B4_2235 at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.547 kb on - strand at 2548.547 kb on - strand at 2548.547 kb on - strand at 2548.567 kb on + strand at 2548.568 kb on - strand at 2548.582 kb on - strand at 2548.642 kb on + strand at 2548.642 kb on + strand at 2548.643 kb on - strand at 2548.643 kb on - strand at 2548.689 kb on + strand at 2548.689 kb on + strand at 2548.689 kb on + strand at 2548.690 kb on - strand at 2548.691 kb on + strand at 2548.691 kb on + strand at 2548.692 kb on - strand at 2548.692 kb on - strand at 2548.739 kb on + strand at 2548.739 kb on + strand at 2548.739 kb on + strand at 2548.739 kb on + strand at 2548.739 kb on + strand at 2548.782 kb on + strand at 2548.783 kb on - strand at 2548.880 kb on + strand at 2548.880 kb on + strand at 2549.170 kb on - strand, within Pf1N1B4_2236 at 2549.170 kb on - strand, within Pf1N1B4_2236 at 2549.170 kb on - strand, within Pf1N1B4_2236 at 2549.178 kb on + strand, within Pf1N1B4_2236 at 2549.178 kb on + strand, within Pf1N1B4_2236 at 2549.179 kb on - strand, within Pf1N1B4_2236 at 2549.179 kb on - strand, within Pf1N1B4_2236 at 2549.352 kb on - strand, within Pf1N1B4_2236 at 2549.352 kb on - strand, within Pf1N1B4_2236 at 2549.352 kb on - strand, within Pf1N1B4_2236 at 2549.352 kb on - strand, within Pf1N1B4_2236 at 2549.494 kb on - strand, within Pf1N1B4_2236
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 2,547,196 + +1.0 2,547,252 - -1.5 2,547,255 + -0.5 2,547,256 - +0.5 2,547,303 - -1.8 2,547,303 - -1.5 2,547,354 - -1.8 2,547,563 + Pf1N1B4_2234 0.32 -2.1 2,547,564 - Pf1N1B4_2234 0.32 +1.5 2,547,691 + Pf1N1B4_2234 0.47 -1.7 2,547,692 - Pf1N1B4_2234 0.47 +1.7 2,547,879 + Pf1N1B4_2234 0.70 -1.2 2,547,879 + Pf1N1B4_2234 0.70 -0.5 2,547,982 + Pf1N1B4_2234 0.82 +0.5 2,547,983 - Pf1N1B4_2234 0.82 +1.1 2,547,983 - Pf1N1B4_2234 0.82 +0.2 2,547,983 - Pf1N1B4_2234 0.82 +0.5 2,547,983 - Pf1N1B4_2234 0.82 -0.0 2,547,994 + Pf1N1B4_2234 0.83 +2.5 2,547,994 + Pf1N1B4_2234 0.83 +1.1 2,547,994 + Pf1N1B4_2234 0.83 +0.6 2,548,001 - Pf1N1B4_2234 0.84 +0.9 2,548,027 - Pf1N1B4_2234 0.87 -2.1 2,548,131 - -0.6 2,548,238 + Pf1N1B4_2235 0.14 +0.5 2,548,238 + Pf1N1B4_2235 0.14 -1.5 2,548,239 - Pf1N1B4_2235 0.14 +2.6 2,548,239 - Pf1N1B4_2235 0.14 +0.9 2,548,266 + Pf1N1B4_2235 0.21 -0.1 2,548,266 + Pf1N1B4_2235 0.21 +0.8 2,548,266 + Pf1N1B4_2235 0.21 +0.5 2,548,301 + Pf1N1B4_2235 0.30 +0.2 2,548,301 + Pf1N1B4_2235 0.30 -1.8 2,548,301 + Pf1N1B4_2235 0.30 +0.1 2,548,301 + Pf1N1B4_2235 0.30 -0.7 2,548,301 + Pf1N1B4_2235 0.30 +1.2 2,548,301 + Pf1N1B4_2235 0.30 +1.0 2,548,301 + Pf1N1B4_2235 0.30 +0.5 2,548,301 + Pf1N1B4_2235 0.30 +0.8 2,548,301 + Pf1N1B4_2235 0.30 -0.5 2,548,301 + Pf1N1B4_2235 0.30 +0.2 2,548,301 + Pf1N1B4_2235 0.30 +1.5 2,548,301 + Pf1N1B4_2235 0.30 -0.8 2,548,301 + Pf1N1B4_2235 0.30 +1.2 2,548,301 + Pf1N1B4_2235 0.30 +1.7 2,548,301 + Pf1N1B4_2235 0.30 +1.2 2,548,301 + Pf1N1B4_2235 0.30 +0.5 2,548,301 + Pf1N1B4_2235 0.30 +0.9 2,548,301 + Pf1N1B4_2235 0.30 -0.7 2,548,301 + Pf1N1B4_2235 0.30 -0.5 2,548,301 + Pf1N1B4_2235 0.30 +0.7 2,548,301 + Pf1N1B4_2235 0.30 -1.1 2,548,301 + Pf1N1B4_2235 0.30 -2.0 2,548,301 + Pf1N1B4_2235 0.30 +0.0 2,548,301 + Pf1N1B4_2235 0.30 -0.7 2,548,301 + Pf1N1B4_2235 0.30 +0.5 2,548,301 + Pf1N1B4_2235 0.30 +0.5 2,548,301 + Pf1N1B4_2235 0.30 +0.1 2,548,302 - Pf1N1B4_2235 0.31 -0.8 2,548,302 - Pf1N1B4_2235 0.31 -1.1 2,548,302 - Pf1N1B4_2235 0.31 -0.1 2,548,302 - Pf1N1B4_2235 0.31 +0.9 2,548,302 - Pf1N1B4_2235 0.31 -0.2 2,548,302 - Pf1N1B4_2235 0.31 -1.1 2,548,302 - Pf1N1B4_2235 0.31 +1.7 2,548,393 + Pf1N1B4_2235 0.54 -0.0 2,548,393 + Pf1N1B4_2235 0.54 +0.5 2,548,393 + Pf1N1B4_2235 0.54 +0.1 2,548,393 + Pf1N1B4_2235 0.54 +0.5 2,548,393 + Pf1N1B4_2235 0.54 +0.5 2,548,393 + Pf1N1B4_2235 0.54 -1.1 2,548,393 + Pf1N1B4_2235 0.54 +1.2 2,548,393 + Pf1N1B4_2235 0.54 +0.1 2,548,393 + Pf1N1B4_2235 0.54 +0.9 2,548,393 + Pf1N1B4_2235 0.54 -0.5 2,548,393 + Pf1N1B4_2235 0.54 +0.2 2,548,393 + Pf1N1B4_2235 0.54 +0.8 2,548,394 - Pf1N1B4_2235 0.55 -1.2 2,548,394 - Pf1N1B4_2235 0.55 +0.5 2,548,394 - Pf1N1B4_2235 0.55 +0.2 2,548,471 + Pf1N1B4_2235 0.74 -0.2 2,548,471 + Pf1N1B4_2235 0.74 -0.5 2,548,471 + Pf1N1B4_2235 0.74 +0.7 2,548,471 + Pf1N1B4_2235 0.74 -0.7 2,548,471 + Pf1N1B4_2235 0.74 -2.3 2,548,546 + +0.5 2,548,546 + +3.1 2,548,546 + +1.2 2,548,546 + -0.1 2,548,546 + +1.8 2,548,546 + +1.2 2,548,546 + +0.5 2,548,546 + +0.3 2,548,546 + +1.2 2,548,547 - +2.1 2,548,547 - +2.5 2,548,547 - +1.2 2,548,567 + -0.8 2,548,568 - +1.8 2,548,582 - +1.2 2,548,642 + +0.8 2,548,642 + +1.4 2,548,643 - +1.0 2,548,643 - +2.1 2,548,689 + +0.9 2,548,689 + -0.5 2,548,689 + -0.0 2,548,690 - -1.5 2,548,691 + -0.5 2,548,691 + -1.1 2,548,692 - -0.1 2,548,692 - +0.5 2,548,739 + +0.6 2,548,739 + -2.3 2,548,739 + +1.2 2,548,739 + +0.5 2,548,739 + +0.2 2,548,782 + +1.2 2,548,783 - +0.1 2,548,880 + +0.1 2,548,880 + +0.5 2,549,170 - Pf1N1B4_2236 0.12 -1.5 2,549,170 - Pf1N1B4_2236 0.12 +0.0 2,549,170 - Pf1N1B4_2236 0.12 -0.4 2,549,178 + Pf1N1B4_2236 0.12 +0.5 2,549,178 + Pf1N1B4_2236 0.12 +0.1 2,549,179 - Pf1N1B4_2236 0.12 +2.1 2,549,179 - Pf1N1B4_2236 0.12 +1.5 2,549,352 - Pf1N1B4_2236 0.18 +0.1 2,549,352 - Pf1N1B4_2236 0.18 +0.5 2,549,352 - Pf1N1B4_2236 0.18 -0.1 2,549,352 - Pf1N1B4_2236 0.18 +1.1 2,549,494 - Pf1N1B4_2236 0.23 -1.1
Or see this region's nucleotide sequence