Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1418 and Pf1N1B4_1419 are separated by 101 nucleotides Pf1N1B4_1419 and Pf1N1B4_1420 are separated by 182 nucleotides Pf1N1B4_1420 and Pf1N1B4_1421 are separated by 107 nucleotides
Pf1N1B4_1418 - Epoxyqueuosine (oQ) reductase QueG, at 1,627,385 to 1,628,449
_1418
Pf1N1B4_1419 - putative membrane protein, at 1,628,551 to 1,629,162
_1419
Pf1N1B4_1420 - 3'-to-5' oligoribonuclease (orn), at 1,629,345 to 1,629,887
_1420
Pf1N1B4_1421 - Ribosome small subunit-stimulated GTPase EngC, at 1,629,995 to 1,631,026
_1421
Position (kb)
1629
1630 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1628.388 kb on - strand at 1628.447 kb on + strand at 1628.448 kb on - strand at 1628.539 kb on - strand at 1628.567 kb on + strand at 1628.568 kb on - strand at 1628.568 kb on - strand at 1628.568 kb on - strand at 1628.663 kb on - strand, within Pf1N1B4_1419 at 1628.672 kb on + strand, within Pf1N1B4_1419 at 1628.672 kb on + strand, within Pf1N1B4_1419 at 1628.672 kb on + strand, within Pf1N1B4_1419 at 1628.673 kb on - strand, within Pf1N1B4_1419 at 1628.673 kb on - strand, within Pf1N1B4_1419 at 1628.673 kb on - strand, within Pf1N1B4_1419 at 1628.673 kb on - strand, within Pf1N1B4_1419 at 1628.673 kb on - strand, within Pf1N1B4_1419 at 1628.687 kb on + strand, within Pf1N1B4_1419 at 1628.721 kb on - strand, within Pf1N1B4_1419 at 1628.737 kb on + strand, within Pf1N1B4_1419 at 1628.737 kb on + strand, within Pf1N1B4_1419 at 1628.738 kb on - strand, within Pf1N1B4_1419 at 1628.819 kb on - strand, within Pf1N1B4_1419 at 1628.975 kb on + strand, within Pf1N1B4_1419 at 1628.976 kb on - strand, within Pf1N1B4_1419 at 1628.976 kb on - strand, within Pf1N1B4_1419 at 1628.976 kb on - strand, within Pf1N1B4_1419 at 1628.976 kb on - strand, within Pf1N1B4_1419 at 1629.146 kb on + strand at 1629.146 kb on + strand at 1629.146 kb on + strand at 1629.147 kb on - strand at 1629.195 kb on - strand at 1629.202 kb on + strand at 1629.203 kb on - strand at 1629.237 kb on - strand at 1629.237 kb on - strand at 1629.274 kb on + strand at 1629.274 kb on + strand at 1629.274 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.280 kb on + strand at 1629.281 kb on - strand at 1629.281 kb on - strand at 1629.281 kb on - strand at 1629.906 kb on - strand at 1629.906 kb on - strand at 1629.918 kb on + strand at 1629.944 kb on + strand at 1629.944 kb on + strand at 1629.965 kb on + strand at 1629.965 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,628,388 - -1.9 1,628,447 + -1.4 1,628,448 - +0.5 1,628,539 - -2.5 1,628,567 + -1.1 1,628,568 - -0.1 1,628,568 - +0.9 1,628,568 - -2.7 1,628,663 - Pf1N1B4_1419 0.18 +0.5 1,628,672 + Pf1N1B4_1419 0.20 +0.5 1,628,672 + Pf1N1B4_1419 0.20 -2.1 1,628,672 + Pf1N1B4_1419 0.20 +0.5 1,628,673 - Pf1N1B4_1419 0.20 -1.0 1,628,673 - Pf1N1B4_1419 0.20 -1.4 1,628,673 - Pf1N1B4_1419 0.20 -2.1 1,628,673 - Pf1N1B4_1419 0.20 -0.7 1,628,673 - Pf1N1B4_1419 0.20 +0.3 1,628,687 + Pf1N1B4_1419 0.22 -0.5 1,628,721 - Pf1N1B4_1419 0.28 +2.0 1,628,737 + Pf1N1B4_1419 0.30 -0.5 1,628,737 + Pf1N1B4_1419 0.30 +2.0 1,628,738 - Pf1N1B4_1419 0.31 -0.5 1,628,819 - Pf1N1B4_1419 0.44 -1.5 1,628,975 + Pf1N1B4_1419 0.69 +0.3 1,628,976 - Pf1N1B4_1419 0.69 +0.9 1,628,976 - Pf1N1B4_1419 0.69 +1.5 1,628,976 - Pf1N1B4_1419 0.69 -2.4 1,628,976 - Pf1N1B4_1419 0.69 -0.5 1,629,146 + -1.1 1,629,146 + -1.5 1,629,146 + -0.9 1,629,147 - -0.5 1,629,195 - -1.9 1,629,202 + +0.5 1,629,203 - +1.0 1,629,237 - -0.7 1,629,237 - +1.0 1,629,274 + +0.5 1,629,274 + -1.3 1,629,274 + -0.5 1,629,280 + -1.5 1,629,280 + -1.5 1,629,280 + +0.5 1,629,280 + +0.4 1,629,280 + +3.0 1,629,281 - -2.4 1,629,281 - +3.3 1,629,281 - -1.3 1,629,906 - +0.9 1,629,906 - -1.1 1,629,918 + -1.1 1,629,944 + +1.5 1,629,944 + -1.9 1,629,965 + -1.4 1,629,965 + +0.5
Or see this region's nucleotide sequence