Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1238 and Pf1N1B4_1239 are separated by 65 nucleotides Pf1N1B4_1239 and Pf1N1B4_1240 are separated by 139 nucleotides
Pf1N1B4_1238 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27), at 1,415,326 to 1,416,819
_1238
Pf1N1B4_1239 - Omega-amino acid--pyruvate aminotransferase (EC 2.6.1.18), at 1,416,885 to 1,418,234
_1239
Pf1N1B4_1240 - LysR family transcriptional regulator PA0133, at 1,418,374 to 1,419,294
_1240
Position (kb)
1416
1417
1418
1419 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1415.959 kb on + strand, within Pf1N1B4_1238 at 1415.959 kb on + strand, within Pf1N1B4_1238 at 1415.960 kb on - strand, within Pf1N1B4_1238 at 1415.960 kb on - strand, within Pf1N1B4_1238 at 1415.960 kb on - strand, within Pf1N1B4_1238 at 1415.960 kb on - strand at 1416.122 kb on + strand, within Pf1N1B4_1238 at 1416.123 kb on - strand, within Pf1N1B4_1238 at 1416.123 kb on - strand, within Pf1N1B4_1238 at 1416.128 kb on + strand, within Pf1N1B4_1238 at 1416.129 kb on - strand, within Pf1N1B4_1238 at 1416.129 kb on - strand, within Pf1N1B4_1238 at 1416.129 kb on - strand, within Pf1N1B4_1238 at 1416.312 kb on + strand, within Pf1N1B4_1238 at 1416.344 kb on + strand, within Pf1N1B4_1238 at 1416.344 kb on + strand, within Pf1N1B4_1238 at 1416.344 kb on + strand, within Pf1N1B4_1238 at 1416.345 kb on - strand, within Pf1N1B4_1238 at 1416.345 kb on - strand, within Pf1N1B4_1238 at 1416.345 kb on - strand, within Pf1N1B4_1238 at 1416.384 kb on + strand, within Pf1N1B4_1238 at 1416.387 kb on + strand at 1416.387 kb on + strand, within Pf1N1B4_1238 at 1416.387 kb on + strand, within Pf1N1B4_1238 at 1416.388 kb on - strand, within Pf1N1B4_1238 at 1416.388 kb on - strand, within Pf1N1B4_1238 at 1416.455 kb on + strand, within Pf1N1B4_1238 at 1416.869 kb on - strand at 1416.869 kb on - strand at 1416.869 kb on - strand at 1416.869 kb on - strand at 1416.869 kb on - strand at 1416.869 kb on - strand at 1416.870 kb on + strand at 1416.871 kb on - strand at 1416.871 kb on - strand at 1416.871 kb on - strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.872 kb on + strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.873 kb on - strand at 1416.997 kb on + strand at 1416.997 kb on + strand at 1416.997 kb on + strand at 1416.998 kb on - strand at 1417.093 kb on + strand, within Pf1N1B4_1239 at 1417.093 kb on + strand, within Pf1N1B4_1239 at 1417.094 kb on - strand, within Pf1N1B4_1239 at 1417.097 kb on + strand, within Pf1N1B4_1239 at 1417.098 kb on - strand, within Pf1N1B4_1239 at 1417.098 kb on - strand, within Pf1N1B4_1239 at 1417.098 kb on - strand, within Pf1N1B4_1239 at 1417.098 kb on - strand, within Pf1N1B4_1239 at 1417.098 kb on - strand, within Pf1N1B4_1239 at 1417.098 kb on - strand, within Pf1N1B4_1239 at 1417.249 kb on + strand, within Pf1N1B4_1239 at 1417.249 kb on + strand, within Pf1N1B4_1239 at 1417.249 kb on + strand, within Pf1N1B4_1239 at 1417.249 kb on + strand, within Pf1N1B4_1239 at 1417.249 kb on + strand, within Pf1N1B4_1239 at 1417.250 kb on - strand, within Pf1N1B4_1239 at 1417.250 kb on - strand, within Pf1N1B4_1239 at 1417.250 kb on - strand, within Pf1N1B4_1239 at 1417.312 kb on + strand, within Pf1N1B4_1239 at 1417.312 kb on + strand, within Pf1N1B4_1239 at 1417.312 kb on + strand, within Pf1N1B4_1239 at 1417.312 kb on + strand, within Pf1N1B4_1239 at 1417.312 kb on + strand, within Pf1N1B4_1239 at 1417.312 kb on + strand, within Pf1N1B4_1239 at 1417.313 kb on - strand, within Pf1N1B4_1239 at 1417.313 kb on - strand, within Pf1N1B4_1239 at 1417.313 kb on - strand, within Pf1N1B4_1239 at 1417.313 kb on - strand, within Pf1N1B4_1239 at 1417.313 kb on - strand, within Pf1N1B4_1239 at 1417.382 kb on + strand, within Pf1N1B4_1239 at 1417.382 kb on + strand, within Pf1N1B4_1239 at 1417.382 kb on + strand, within Pf1N1B4_1239 at 1417.382 kb on + strand, within Pf1N1B4_1239 at 1417.382 kb on + strand, within Pf1N1B4_1239 at 1417.382 kb on + strand, within Pf1N1B4_1239 at 1417.383 kb on - strand, within Pf1N1B4_1239 at 1417.383 kb on - strand, within Pf1N1B4_1239 at 1417.383 kb on - strand, within Pf1N1B4_1239 at 1417.383 kb on - strand, within Pf1N1B4_1239 at 1417.383 kb on - strand, within Pf1N1B4_1239 at 1417.383 kb on - strand, within Pf1N1B4_1239 at 1417.383 kb on - strand, within Pf1N1B4_1239 at 1417.383 kb on - strand, within Pf1N1B4_1239 at 1417.511 kb on - strand, within Pf1N1B4_1239 at 1417.511 kb on - strand, within Pf1N1B4_1239 at 1417.835 kb on - strand, within Pf1N1B4_1239 at 1417.835 kb on - strand, within Pf1N1B4_1239 at 1417.982 kb on - strand, within Pf1N1B4_1239 at 1417.982 kb on - strand, within Pf1N1B4_1239 at 1418.074 kb on + strand, within Pf1N1B4_1239 at 1418.075 kb on - strand, within Pf1N1B4_1239 at 1418.165 kb on - strand at 1418.165 kb on - strand at 1418.165 kb on - strand at 1418.256 kb on + strand at 1418.290 kb on + strand at 1418.306 kb on + strand at 1418.306 kb on + strand at 1418.306 kb on + strand at 1418.608 kb on + strand, within Pf1N1B4_1240 at 1418.609 kb on - strand, within Pf1N1B4_1240 at 1418.627 kb on + strand, within Pf1N1B4_1240 at 1418.627 kb on + strand, within Pf1N1B4_1240 at 1418.628 kb on - strand, within Pf1N1B4_1240 at 1418.628 kb on - strand, within Pf1N1B4_1240 at 1418.628 kb on - strand, within Pf1N1B4_1240 at 1418.628 kb on - strand, within Pf1N1B4_1240 at 1418.628 kb on - strand, within Pf1N1B4_1240 at 1418.630 kb on + strand, within Pf1N1B4_1240 at 1418.630 kb on + strand, within Pf1N1B4_1240 at 1418.630 kb on + strand, within Pf1N1B4_1240 at 1418.630 kb on + strand, within Pf1N1B4_1240 at 1418.631 kb on - strand, within Pf1N1B4_1240 at 1418.631 kb on - strand, within Pf1N1B4_1240 at 1418.784 kb on - strand, within Pf1N1B4_1240 at 1418.784 kb on - strand, within Pf1N1B4_1240 at 1418.784 kb on - strand, within Pf1N1B4_1240 at 1418.894 kb on - strand, within Pf1N1B4_1240 at 1418.905 kb on + strand, within Pf1N1B4_1240 at 1418.905 kb on + strand, within Pf1N1B4_1240 at 1418.926 kb on + strand, within Pf1N1B4_1240 at 1418.926 kb on + strand, within Pf1N1B4_1240 at 1418.927 kb on - strand, within Pf1N1B4_1240 at 1418.927 kb on - strand at 1418.927 kb on - strand, within Pf1N1B4_1240 at 1418.927 kb on - strand, within Pf1N1B4_1240 at 1418.953 kb on + strand, within Pf1N1B4_1240 at 1418.953 kb on + strand, within Pf1N1B4_1240 at 1418.953 kb on + strand, within Pf1N1B4_1240 at 1418.953 kb on + strand, within Pf1N1B4_1240 at 1418.953 kb on + strand, within Pf1N1B4_1240 at 1418.954 kb on - strand, within Pf1N1B4_1240 at 1418.954 kb on - strand, within Pf1N1B4_1240 at 1418.954 kb on - strand, within Pf1N1B4_1240 at 1418.957 kb on + strand, within Pf1N1B4_1240 at 1418.957 kb on + strand at 1418.957 kb on + strand, within Pf1N1B4_1240 at 1418.958 kb on - strand, within Pf1N1B4_1240 at 1418.958 kb on - strand, within Pf1N1B4_1240 at 1418.958 kb on - strand, within Pf1N1B4_1240 at 1418.958 kb on - strand at 1418.958 kb on - strand, within Pf1N1B4_1240 at 1419.046 kb on + strand, within Pf1N1B4_1240 at 1419.047 kb on - strand at 1419.118 kb on + strand, within Pf1N1B4_1240 at 1419.118 kb on + strand, within Pf1N1B4_1240 at 1419.118 kb on + strand, within Pf1N1B4_1240 at 1419.127 kb on + strand, within Pf1N1B4_1240 at 1419.142 kb on + strand, within Pf1N1B4_1240 at 1419.202 kb on + strand, within Pf1N1B4_1240 at 1419.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,415,959 + Pf1N1B4_1238 0.42 +0.4 1,415,959 + Pf1N1B4_1238 0.42 +1.1 1,415,960 - Pf1N1B4_1238 0.42 +0.9 1,415,960 - Pf1N1B4_1238 0.42 -0.6 1,415,960 - Pf1N1B4_1238 0.42 -0.6 1,415,960 - -0.6 1,416,122 + Pf1N1B4_1238 0.53 -0.1 1,416,123 - Pf1N1B4_1238 0.53 +0.4 1,416,123 - Pf1N1B4_1238 0.53 -0.6 1,416,128 + Pf1N1B4_1238 0.54 -0.1 1,416,129 - Pf1N1B4_1238 0.54 -2.5 1,416,129 - Pf1N1B4_1238 0.54 -0.6 1,416,129 - Pf1N1B4_1238 0.54 -1.1 1,416,312 + Pf1N1B4_1238 0.66 +2.9 1,416,344 + Pf1N1B4_1238 0.68 -1.6 1,416,344 + Pf1N1B4_1238 0.68 -1.5 1,416,344 + Pf1N1B4_1238 0.68 -2.0 1,416,345 - Pf1N1B4_1238 0.68 +0.4 1,416,345 - Pf1N1B4_1238 0.68 +0.4 1,416,345 - Pf1N1B4_1238 0.68 +0.8 1,416,384 + Pf1N1B4_1238 0.71 -0.4 1,416,387 + +1.9 1,416,387 + Pf1N1B4_1238 0.71 +0.8 1,416,387 + Pf1N1B4_1238 0.71 -2.0 1,416,388 - Pf1N1B4_1238 0.71 +0.2 1,416,388 - Pf1N1B4_1238 0.71 +1.4 1,416,455 + Pf1N1B4_1238 0.76 -1.2 1,416,869 - -0.6 1,416,869 - -0.2 1,416,869 - -0.9 1,416,869 - +0.4 1,416,869 - +1.8 1,416,869 - -0.6 1,416,870 + -0.2 1,416,871 - -0.6 1,416,871 - +0.6 1,416,871 - -0.1 1,416,872 + +0.9 1,416,872 + -2.0 1,416,872 + +0.7 1,416,872 + +0.7 1,416,872 + -0.1 1,416,872 + -0.6 1,416,872 + -0.6 1,416,872 + +0.4 1,416,872 + -1.2 1,416,872 + -0.6 1,416,872 + +2.9 1,416,872 + -0.2 1,416,872 + -1.2 1,416,872 + +0.9 1,416,872 + +1.8 1,416,872 + +0.2 1,416,872 + -0.1 1,416,872 + -1.2 1,416,872 + +0.4 1,416,872 + +0.7 1,416,872 + +1.1 1,416,872 + -0.1 1,416,872 + -2.5 1,416,873 - -0.2 1,416,873 - -0.6 1,416,873 - +0.6 1,416,873 - +0.4 1,416,873 - +0.8 1,416,873 - +0.0 1,416,873 - -0.6 1,416,873 - -1.6 1,416,873 - -2.6 1,416,873 - +0.4 1,416,873 - -0.3 1,416,873 - +0.8 1,416,873 - +0.4 1,416,873 - -1.2 1,416,873 - -1.5 1,416,873 - -0.2 1,416,873 - +0.8 1,416,997 + +0.9 1,416,997 + -0.6 1,416,997 + +0.6 1,416,998 - -0.4 1,417,093 + Pf1N1B4_1239 0.15 -1.6 1,417,093 + Pf1N1B4_1239 0.15 +1.4 1,417,094 - Pf1N1B4_1239 0.15 -0.6 1,417,097 + Pf1N1B4_1239 0.16 -1.2 1,417,098 - Pf1N1B4_1239 0.16 +0.8 1,417,098 - Pf1N1B4_1239 0.16 -1.0 1,417,098 - Pf1N1B4_1239 0.16 +2.9 1,417,098 - Pf1N1B4_1239 0.16 -0.1 1,417,098 - Pf1N1B4_1239 0.16 -2.2 1,417,098 - Pf1N1B4_1239 0.16 +0.6 1,417,249 + Pf1N1B4_1239 0.27 +0.9 1,417,249 + Pf1N1B4_1239 0.27 -0.0 1,417,249 + Pf1N1B4_1239 0.27 +1.6 1,417,249 + Pf1N1B4_1239 0.27 +1.1 1,417,249 + Pf1N1B4_1239 0.27 -2.0 1,417,250 - Pf1N1B4_1239 0.27 +0.5 1,417,250 - Pf1N1B4_1239 0.27 -0.2 1,417,250 - Pf1N1B4_1239 0.27 +1.6 1,417,312 + Pf1N1B4_1239 0.32 -0.6 1,417,312 + Pf1N1B4_1239 0.32 +0.4 1,417,312 + Pf1N1B4_1239 0.32 -2.0 1,417,312 + Pf1N1B4_1239 0.32 +3.2 1,417,312 + Pf1N1B4_1239 0.32 +1.4 1,417,312 + Pf1N1B4_1239 0.32 -0.8 1,417,313 - Pf1N1B4_1239 0.32 -0.6 1,417,313 - Pf1N1B4_1239 0.32 +0.4 1,417,313 - Pf1N1B4_1239 0.32 +0.8 1,417,313 - Pf1N1B4_1239 0.32 +2.5 1,417,313 - Pf1N1B4_1239 0.32 -1.6 1,417,382 + Pf1N1B4_1239 0.37 -0.1 1,417,382 + Pf1N1B4_1239 0.37 -1.5 1,417,382 + Pf1N1B4_1239 0.37 +1.0 1,417,382 + Pf1N1B4_1239 0.37 -2.0 1,417,382 + Pf1N1B4_1239 0.37 +3.1 1,417,382 + Pf1N1B4_1239 0.37 +0.9 1,417,383 - Pf1N1B4_1239 0.37 -1.2 1,417,383 - Pf1N1B4_1239 0.37 +0.4 1,417,383 - Pf1N1B4_1239 0.37 -2.0 1,417,383 - Pf1N1B4_1239 0.37 +1.1 1,417,383 - Pf1N1B4_1239 0.37 -0.6 1,417,383 - Pf1N1B4_1239 0.37 +0.7 1,417,383 - Pf1N1B4_1239 0.37 -0.2 1,417,383 - Pf1N1B4_1239 0.37 +0.4 1,417,511 - Pf1N1B4_1239 0.46 +0.4 1,417,511 - Pf1N1B4_1239 0.46 +0.8 1,417,835 - Pf1N1B4_1239 0.70 -1.2 1,417,835 - Pf1N1B4_1239 0.70 +0.9 1,417,982 - Pf1N1B4_1239 0.81 -0.4 1,417,982 - Pf1N1B4_1239 0.81 +1.9 1,418,074 + Pf1N1B4_1239 0.88 -0.6 1,418,075 - Pf1N1B4_1239 0.88 +1.9 1,418,165 - -0.6 1,418,165 - +0.4 1,418,165 - +0.4 1,418,256 + -0.2 1,418,290 + +2.4 1,418,306 + +0.4 1,418,306 + -0.2 1,418,306 + -0.2 1,418,608 + Pf1N1B4_1240 0.25 -0.2 1,418,609 - Pf1N1B4_1240 0.26 +0.9 1,418,627 + Pf1N1B4_1240 0.27 +3.8 1,418,627 + Pf1N1B4_1240 0.27 -1.6 1,418,628 - Pf1N1B4_1240 0.28 -1.1 1,418,628 - Pf1N1B4_1240 0.28 +2.5 1,418,628 - Pf1N1B4_1240 0.28 +0.9 1,418,628 - Pf1N1B4_1240 0.28 +0.2 1,418,628 - Pf1N1B4_1240 0.28 +0.4 1,418,630 + Pf1N1B4_1240 0.28 +0.2 1,418,630 + Pf1N1B4_1240 0.28 -0.6 1,418,630 + Pf1N1B4_1240 0.28 -0.9 1,418,630 + Pf1N1B4_1240 0.28 -2.0 1,418,631 - Pf1N1B4_1240 0.28 -0.2 1,418,631 - Pf1N1B4_1240 0.28 -0.0 1,418,784 - Pf1N1B4_1240 0.45 +0.8 1,418,784 - Pf1N1B4_1240 0.45 +1.4 1,418,784 - Pf1N1B4_1240 0.45 +0.4 1,418,894 - Pf1N1B4_1240 0.56 +0.8 1,418,905 + Pf1N1B4_1240 0.58 +0.6 1,418,905 + Pf1N1B4_1240 0.58 +3.7 1,418,926 + Pf1N1B4_1240 0.60 +0.5 1,418,926 + Pf1N1B4_1240 0.60 -2.5 1,418,927 - Pf1N1B4_1240 0.60 +1.4 1,418,927 - -0.2 1,418,927 - Pf1N1B4_1240 0.60 +0.9 1,418,927 - Pf1N1B4_1240 0.60 +0.4 1,418,953 + Pf1N1B4_1240 0.63 -0.6 1,418,953 + Pf1N1B4_1240 0.63 +0.6 1,418,953 + Pf1N1B4_1240 0.63 -1.2 1,418,953 + Pf1N1B4_1240 0.63 -2.2 1,418,953 + Pf1N1B4_1240 0.63 +0.4 1,418,954 - Pf1N1B4_1240 0.63 -3.0 1,418,954 - Pf1N1B4_1240 0.63 -1.6 1,418,954 - Pf1N1B4_1240 0.63 +0.4 1,418,957 + Pf1N1B4_1240 0.63 -0.5 1,418,957 + +0.9 1,418,957 + Pf1N1B4_1240 0.63 +1.9 1,418,958 - Pf1N1B4_1240 0.63 -1.5 1,418,958 - Pf1N1B4_1240 0.63 +0.5 1,418,958 - Pf1N1B4_1240 0.63 +1.4 1,418,958 - -0.6 1,418,958 - Pf1N1B4_1240 0.63 +1.8 1,419,046 + Pf1N1B4_1240 0.73 -0.6 1,419,047 - +1.4 1,419,118 + Pf1N1B4_1240 0.81 -3.2 1,419,118 + Pf1N1B4_1240 0.81 -1.5 1,419,118 + Pf1N1B4_1240 0.81 -0.2 1,419,127 + Pf1N1B4_1240 0.82 +0.5 1,419,142 + Pf1N1B4_1240 0.83 +0.9 1,419,202 + Pf1N1B4_1240 0.90 +1.1 1,419,203 - -0.1
Or see this region's nucleotide sequence