Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1131 and Pf1N1B4_1132 are separated by 20 nucleotides Pf1N1B4_1132 and Pf1N1B4_1133 are separated by 12 nucleotides Pf1N1B4_1133 and Pf1N1B4_1134 overlap by 4 nucleotides
Pf1N1B4_1131 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,269,525 to 1,270,241
_1131
Pf1N1B4_1132 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) / Osmotic adaptation, at 1,270,262 to 1,271,155
_1132
Pf1N1B4_1133 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,271,168 to 1,271,821
_1133
Pf1N1B4_1134 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 1,271,818 to 1,272,975
_1134
Position (kb)
1270
1271
1272 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1269.284 kb on - strand at 1269.284 kb on - strand at 1269.287 kb on + strand at 1269.287 kb on + strand at 1269.287 kb on + strand at 1269.288 kb on - strand at 1269.288 kb on - strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.301 kb on + strand at 1269.301 kb on + strand at 1269.301 kb on + strand at 1269.301 kb on + strand at 1269.302 kb on - strand at 1269.313 kb on + strand at 1269.313 kb on + strand at 1269.313 kb on + strand at 1269.313 kb on + strand at 1269.314 kb on - strand at 1269.314 kb on - strand at 1269.352 kb on + strand at 1269.352 kb on + strand at 1269.353 kb on - strand at 1269.399 kb on - strand at 1269.422 kb on - strand at 1269.537 kb on + strand at 1269.553 kb on + strand at 1269.553 kb on + strand at 1269.554 kb on - strand at 1269.645 kb on + strand, within Pf1N1B4_1131 at 1269.697 kb on + strand, within Pf1N1B4_1131 at 1269.697 kb on + strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.806 kb on - strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.901 kb on - strand, within Pf1N1B4_1131 at 1269.901 kb on - strand, within Pf1N1B4_1131 at 1269.977 kb on - strand, within Pf1N1B4_1131 at 1270.053 kb on - strand, within Pf1N1B4_1131 at 1270.239 kb on + strand at 1270.239 kb on + strand at 1270.239 kb on + strand at 1270.240 kb on - strand at 1270.240 kb on - strand at 1270.240 kb on - strand at 1270.240 kb on - strand at 1270.278 kb on + strand at 1270.278 kb on + strand at 1270.278 kb on + strand at 1270.279 kb on - strand at 1270.279 kb on - strand at 1270.281 kb on + strand at 1270.281 kb on + strand at 1270.281 kb on + strand at 1270.282 kb on - strand at 1270.282 kb on - strand at 1270.282 kb on - strand at 1270.284 kb on + strand at 1270.285 kb on - strand at 1270.285 kb on - strand at 1270.285 kb on - strand at 1270.398 kb on - strand, within Pf1N1B4_1132 at 1270.415 kb on + strand, within Pf1N1B4_1132 at 1270.416 kb on - strand, within Pf1N1B4_1132 at 1270.523 kb on + strand, within Pf1N1B4_1132 at 1270.524 kb on - strand, within Pf1N1B4_1132 at 1270.524 kb on - strand, within Pf1N1B4_1132 at 1270.524 kb on - strand, within Pf1N1B4_1132 at 1270.634 kb on + strand, within Pf1N1B4_1132 at 1270.634 kb on + strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.670 kb on + strand, within Pf1N1B4_1132 at 1270.854 kb on - strand, within Pf1N1B4_1132 at 1270.854 kb on - strand, within Pf1N1B4_1132 at 1270.896 kb on - strand at 1270.896 kb on - strand, within Pf1N1B4_1132 at 1270.896 kb on - strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.998 kb on - strand, within Pf1N1B4_1132 at 1271.396 kb on + strand, within Pf1N1B4_1133 at 1271.397 kb on - strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.679 kb on + strand, within Pf1N1B4_1133 at 1271.679 kb on + strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1272.054 kb on + strand at 1272.054 kb on + strand, within Pf1N1B4_1134 at 1272.055 kb on - strand, within Pf1N1B4_1134 at 1272.055 kb on - strand, within Pf1N1B4_1134 at 1272.064 kb on + strand, within Pf1N1B4_1134 at 1272.065 kb on - strand, within Pf1N1B4_1134 at 1272.065 kb on - strand, within Pf1N1B4_1134
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,269,284 - +0.9 1,269,284 - +1.3 1,269,287 + +1.3 1,269,287 + -0.4 1,269,287 + +0.7 1,269,288 - +1.9 1,269,288 - -0.1 1,269,297 + +1.9 1,269,297 + +0.0 1,269,297 + +0.3 1,269,297 + -0.1 1,269,297 + -0.3 1,269,297 + +0.6 1,269,301 + +0.3 1,269,301 + +0.7 1,269,301 + +1.7 1,269,301 + +1.3 1,269,302 - +0.9 1,269,313 + -0.7 1,269,313 + +0.1 1,269,313 + -1.7 1,269,313 + -0.4 1,269,314 - +1.1 1,269,314 - -1.3 1,269,352 + +1.6 1,269,352 + -0.2 1,269,353 - +1.4 1,269,399 - +0.3 1,269,422 - +0.3 1,269,537 + +0.7 1,269,553 + -0.7 1,269,553 + -1.4 1,269,554 - +2.8 1,269,645 + Pf1N1B4_1131 0.17 +1.4 1,269,697 + Pf1N1B4_1131 0.24 +1.8 1,269,697 + Pf1N1B4_1131 0.24 +1.0 1,269,698 - Pf1N1B4_1131 0.24 +0.3 1,269,698 - Pf1N1B4_1131 0.24 +0.0 1,269,698 - Pf1N1B4_1131 0.24 +1.3 1,269,698 - Pf1N1B4_1131 0.24 -0.3 1,269,698 - Pf1N1B4_1131 0.24 +2.1 1,269,806 - Pf1N1B4_1131 0.39 +0.3 1,269,900 + Pf1N1B4_1131 0.52 -0.9 1,269,900 + Pf1N1B4_1131 0.52 +1.5 1,269,900 + Pf1N1B4_1131 0.52 -0.8 1,269,900 + Pf1N1B4_1131 0.52 -2.0 1,269,900 + Pf1N1B4_1131 0.52 +0.6 1,269,901 - Pf1N1B4_1131 0.52 -0.6 1,269,901 - Pf1N1B4_1131 0.52 -1.2 1,269,977 - Pf1N1B4_1131 0.63 -0.0 1,270,053 - Pf1N1B4_1131 0.74 -0.7 1,270,239 + -2.3 1,270,239 + -0.7 1,270,239 + -0.5 1,270,240 - -3.0 1,270,240 - -0.3 1,270,240 - +0.3 1,270,240 - +1.0 1,270,278 + +1.8 1,270,278 + -0.5 1,270,278 + -0.3 1,270,279 - +2.3 1,270,279 - +1.5 1,270,281 + +2.3 1,270,281 + +0.3 1,270,281 + -0.7 1,270,282 - -0.3 1,270,282 - -1.3 1,270,282 - +0.9 1,270,284 + -1.7 1,270,285 - -0.3 1,270,285 - +0.9 1,270,285 - -1.3 1,270,398 - Pf1N1B4_1132 0.15 +1.3 1,270,415 + Pf1N1B4_1132 0.17 -2.0 1,270,416 - Pf1N1B4_1132 0.17 +0.7 1,270,523 + Pf1N1B4_1132 0.29 +1.9 1,270,524 - Pf1N1B4_1132 0.29 +1.3 1,270,524 - Pf1N1B4_1132 0.29 +1.5 1,270,524 - Pf1N1B4_1132 0.29 +0.7 1,270,634 + Pf1N1B4_1132 0.42 -0.0 1,270,634 + Pf1N1B4_1132 0.42 +1.7 1,270,635 - Pf1N1B4_1132 0.42 +3.3 1,270,635 - Pf1N1B4_1132 0.42 -0.0 1,270,635 - Pf1N1B4_1132 0.42 +0.3 1,270,635 - Pf1N1B4_1132 0.42 +0.3 1,270,670 + Pf1N1B4_1132 0.46 -2.0 1,270,854 - Pf1N1B4_1132 0.66 +2.3 1,270,854 - Pf1N1B4_1132 0.66 -2.3 1,270,896 - +0.3 1,270,896 - Pf1N1B4_1132 0.71 -1.3 1,270,896 - Pf1N1B4_1132 0.71 +0.7 1,270,955 + Pf1N1B4_1132 0.78 -1.3 1,270,955 + Pf1N1B4_1132 0.78 +0.7 1,270,955 + Pf1N1B4_1132 0.78 -1.0 1,270,955 + Pf1N1B4_1132 0.78 -0.5 1,270,955 + +1.3 1,270,955 + Pf1N1B4_1132 0.78 +0.6 1,270,955 + Pf1N1B4_1132 0.78 +2.9 1,270,955 + Pf1N1B4_1132 0.78 +0.7 1,270,955 + Pf1N1B4_1132 0.78 -1.1 1,270,956 - Pf1N1B4_1132 0.78 +0.5 1,270,956 - Pf1N1B4_1132 0.78 +0.6 1,270,956 - Pf1N1B4_1132 0.78 +0.7 1,270,956 - Pf1N1B4_1132 0.78 -1.2 1,270,967 + Pf1N1B4_1132 0.79 -0.1 1,270,967 + Pf1N1B4_1132 0.79 -1.7 1,270,967 + Pf1N1B4_1132 0.79 -2.3 1,270,998 - Pf1N1B4_1132 0.82 -1.7 1,271,396 + Pf1N1B4_1133 0.35 +0.8 1,271,397 - Pf1N1B4_1133 0.35 +0.0 1,271,483 + Pf1N1B4_1133 0.48 +0.6 1,271,483 + +2.9 1,271,483 + Pf1N1B4_1133 0.48 -1.3 1,271,483 + Pf1N1B4_1133 0.48 -0.7 1,271,483 + Pf1N1B4_1133 0.48 -0.1 1,271,484 - Pf1N1B4_1133 0.48 -0.9 1,271,484 - Pf1N1B4_1133 0.48 +1.3 1,271,484 - Pf1N1B4_1133 0.48 -0.7 1,271,484 - Pf1N1B4_1133 0.48 +1.0 1,271,484 - Pf1N1B4_1133 0.48 -0.7 1,271,484 - Pf1N1B4_1133 0.48 -0.2 1,271,679 + Pf1N1B4_1133 0.78 -1.0 1,271,679 + Pf1N1B4_1133 0.78 +1.3 1,271,680 - Pf1N1B4_1133 0.78 +1.1 1,271,680 - Pf1N1B4_1133 0.78 +0.5 1,271,680 - Pf1N1B4_1133 0.78 +1.6 1,272,054 + +1.3 1,272,054 + Pf1N1B4_1134 0.20 -1.3 1,272,055 - Pf1N1B4_1134 0.20 -0.7 1,272,055 - Pf1N1B4_1134 0.20 -0.5 1,272,064 + Pf1N1B4_1134 0.21 -1.7 1,272,065 - Pf1N1B4_1134 0.21 -1.3 1,272,065 - Pf1N1B4_1134 0.21 +2.1
Or see this region's nucleotide sequence